Mouse MCP3 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse MCP3 with a sensitivity of 0.56 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
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Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.78 - 1600 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.56 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity (By similarity).
Alternative names
Fic, Mcp3, Scya7, Ccl7, C-C motif chemokine 7, Intercrine/chemokine MARC, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, Protein FIC, Small-inducible cytokine A7, MCP-3
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Mouse MCP3 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse MCP3 with a sensitivity of 0.56 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.78 - 1600 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.56 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.24 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 2.58 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 91 - 99 % |
Sample type EDTA Plasma | Average % = 93 | Range 88 - 96 % |
Sample type Citrate plasma | Average % = 98 | Range 94 - 102 % |
Sample type Cell culture media | Average % = 89 | Range 89 - 91 % |
Sample type Plasma | Average % = 85 | Range 80 - 87 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
MCP3 (CCL7) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MCP3 (CCL7) protein in mouse serum, plasma and cell culture supernatants.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
MCP3, also known as CCL7, belongs to the C-C chemokine family. MCP3 attracts monocytes, eosinophils, NK cells, T-lymphocytes, but not neutrophils. The ability of MCP3 to interact with various immune cell types are explained by the molecule’s ability to interact with various chemokine receptors. The chemokines known to interact with MCP3 include CCR1, CCR2, and CCR3. MCP3 also augments monocyte anti-tumor activity, and has been implicated in various studies investigating cancer metastasis.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target MCP-3 also referred to as CCL7 is a chemotactic cytokine belonging to the CC chemokine family. Its molecular weight is approximately 11 kDa. MCP-3 is largely expressed by various cell types including monocytes lymphocytes and endothelial cells. This protein plays an important role in immune system signaling. MCP-3 exhibits chemotactic activity for monocytes T lymphocytes and natural killer (NK) cells contributing to immune cell recruitment at sites of inflammation.
- Biological function summary
MCP-3 interacts with several chemokine receptors such as CCR1 CCR2 and CCR3 allowing for diverse roles in immune response and inflammation. It functions as both a monomer and in conjunction with other chemokines to form heterodimers aiding in the modulation and fine-tuning of the immune response. The presence of MCP-3 in inflammatory tissues indicates its contribution to directing leukocyte migration and positioning therefore supporting both innate and adaptive immunity.
- Pathways
MCP-3 is a component of the chemokine signaling pathway and is significant in the regulation of immune surveillance. It interacts closely with proteins such as CCL2 and CCL3 all of which bind to similar receptors like CCR2 and CCR5. MCP-3's involvement in these pathways supports cellular responses to inflammation and infection and its activity has a direct impact on the mobilization and activation of immune cells.
- Associated diseases and disorders
MCP-3 shows associations with conditions marked by inflammation and immune system dysregulation including rheumatoid arthritis and asthma. The protein's influence in these disorders links to other chemokines like MCP-1 (CCL2) which also recruits monocytes and contributes to chronic inflammation. Disruption in MCP-3 expression or function can aggravate disease states by altering the balance of leukocyte trafficking and exacerbating inflammatory responses.
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3 product images
Sandwich ELISA - Mouse MCP3 ELISA Kit, Fluorescent (ab229391)
Example of mouse MCP3 (CCL7) standard curve in Sample Diluent NS.
The MCP3 (CCL7) standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse MCP3 ELISA Kit, Fluorescent (ab229391)
Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.
The 1X dilution was 25% for the mouse serum and all of the mouse plasmas. Interpolated data values (mean +/- SD, n = 2) are graphed.
Sandwich ELISA - Mouse MCP3 ELISA Kit, Fluorescent (ab229391)
Titration of mouse stimulated (5 µg/mL LPS, 48 hrs (+)) and unstimulated (-) Raw 264.7 supernatant, and unstimulated (-) D10.G4.1 supernatant.
A blank cell culture media control was also analyzed, and was negative. The 1X dilution was 3.13% for the stimulated and unstimulated Raw 264.7 supernatants, and 25.0% for the unstimulated D10.G4.1 supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.
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