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AB100723

Mouse MCP5 ELISA Kit

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Mouse MCP5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse MCP5 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Mcp5, Scya12, Ccl12, C-C motif chemokine 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Small-inducible cytokine A12, MCP-5

2 Images
Sandwich ELISA - Mouse MCP5 ELISA Kit (AB100723)
  • sELISA

Unknown

Sandwich ELISA - Mouse MCP5 ELISA Kit (AB100723)

Representative standard curve using ab100723

Sandwich ELISA - Mouse MCP5 ELISA Kit (AB100723)
  • sELISA

Unknown

Sandwich ELISA - Mouse MCP5 ELISA Kit (AB100723)

Representative standard curve using ab100723

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 0.5 pg/mL

Range

0.41 - 100 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MCP5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP5 in serum, plasma and cell culture supernates.

This assay employs an antibody specific for mouse MCP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 105 %", "average": "= 95.34" }, { "sample": "Serum", "range": "81 - 103 %", "average": "= 92.42" }, { "sample": "Plasma", "range": "82 - 104 %", "average": "= 94.14" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100723-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100723-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100723-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100723-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse MCP5 Standard (lyophilized)", "number":"AB100723-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100723-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"160X HRP-Streptavidin Concentrate", "number":"AB100723-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MCP5 Microplate (12 x 8 wells)", "number":"AB100723-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse MCP5", "number":"AB100723-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100723-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-5 also known as CCL12 is a chemokine protein that plays a role in immune response by recruiting and activating leukocytes. It has a molecular mass of approximately 12 kDa. This protein is expressed mainly in tissues related to the immune system including the spleen and liver. MCP-5 exhibits a sequence homology with other members of the C-C chemokine family and shares functional similarities with MCP-1.
Biological function summary

MCP-5 takes part in promoting the migration of monocytes and lymphocytes to sites of inflammation. This chemokine is not a part of a complex but interacts with specific receptors on target cells such as CCR2. Through these interactions MCP-5 helps mediate a range of immune responses enhancing both innate and adaptive immune activities. It also contributes to the clearance of pathogens by guiding leukocytes to infection sites.

Pathways

MCP-5 plays an important role in the chemokine signaling pathway and the inflammatory response pathway. Through chemokine signaling MCP-5 interacts with CCR2 facilitating the movement of immune cells to areas of tissue damage or infection. This regulation of cell traffic is essential for maintaining homeostasis and responding to pathological changes in tissues. Another protein connected to MCP-5 in these pathways is MCP-3 which shares overlapping functions in immune cell recruitment.

MCP-5 has been implicated in conditions such as asthma and rheumatoid arthritis. In these disorders MCP-5 contributes to the recruitment of inflammatory cells worsening symptoms. It also shows a connection with other proteins like MCP-1 which similarly aids in the movement of monocytes to inflammatory sites. The presence and activity of MCP-5 and its related proteins can therefore represent potential targets for therapeutic interventions in controlling excessive inflammatory responses.
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Product protocols

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Target data

Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
See full target information Ccl12
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websiteProtocolBooklet
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