Mouse MDC ELISA Kit (CCL22)
- Recombinant
- SimpleStep
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(2 Publications)
Mouse MDC ELISA Kit (CCL22) is a single-wash 90-min Simplestep used to quantify Mouse MDC (CCL22) with a sensitivity of 1.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Abcd1, Scya22, Ccl22, C-C motif chemokine 22, Activated B and dendritic cell-derived, CC chemokine ABCD-1, Small-inducible cytokine A22
- sELISA
Unknown
Sandwich ELISA - Mouse MDC ELISA Kit (CCL22) (AB204525)
Mouse MDC standard curve comparison data.
Standard curve comparison between mouse MDC SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Mouse MDC ELISA Kit (CCL22) (AB204525)
Example of MDC standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse MDC ELISA Kit (CCL22) (AB204525)
Interpolated concentrations of MDC in mouse serum, plasma (citrate), and plasma (EDTA).
The concentrations of MDC were measured in duplicate and interpolated from the MDC standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MDC concentration was determined to be 333.6 pg/mL in mouse serum, 247.6 pg/mL in mouse plasma (Citrate), and 229.6 pg/mL in mouse plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Mouse MDC ELISA Kit (CCL22) (AB204525)
Comparison of secreted MDC in unstimulated and PMA/PHA-stimulated J774A.1 Cells.
J7774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. MDC was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated J774A.1 and cell culture media. Measured values were interpolated from the MDC Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 2.9 pg/mL unstimulated, 419.5 pg/mL stimulated, and undetectable in media.
Reactivity data
Product details
Mouse MDC ELISA Kit (CCL22) (ab204525) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MDC (CCL22) protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MDC (CCL22) with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Macrophage-derived chemokine (MDC), also known as C-C motif chemokine 22 (CCL22), is a secreted cytokine and member of the intercrine beta (chemokine CC) family. It is highly expressed in macrophage and in monocyte-derived dendritic cells. CCL22 is a chemotactic for monocytes, dendritic cells and natural killer cells and potent chemoattractant for chronically activated T-lymphocytes.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MDC interacts with immune cells to mediate responses such as chemotaxis. It binds to the CCR4 receptor which is found on T cells and other immune cells. MDC does not require complex formation to function; it works independently to attract specific cell types. This chemokine provides a way for immune cells to navigate to sites of inflammation or infection.
Pathways
MDC plays a significant role in inflammatory and immune response pathways. It is integrally linked with the chemokine signaling pathway where it works along with other chemokines to guide immune responses. MDC is related to proteins like CCR4 and other chemokines in these pathways facilitating communication between cells during immune responses and maintaining homeostasis.
Product protocols
- Visit the General protocols
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- Download websiteProtocolBooklet|en
Target data
Publications (2)
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Clinical cancer research : an official journal of the American Association for Cancer Research 30:4450-4463 PubMed38691100
2024
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Molecular medicine reports 19:4195-4204 PubMed30896872
2019
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Unspecified reactive species
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