Mouse MDC ELISA Kit (Macrophage Derived Chemokine) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse MDC (Macrophage Derived Chemokine) in Mouse in Plasma, Cell culture supernatant, Serum samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 2 - 600 pg/mL
- Sensitivity
- = 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses.
Alternative names
Abcd1, Scya22, Ccl22, C-C motif chemokine 22, Activated B and dendritic cell-derived, CC chemokine ABCD-1, Small-inducible cytokine A22
What's included?
Recommended products
Mouse MDC ELISA Kit (Macrophage Derived Chemokine) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse MDC (Macrophage Derived Chemokine) in Mouse in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 2 - 600 pg/mL
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 2 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 108.6 | Range 103 - 117 % |
Sample type Plasma | Average % = 103 | Range 94 - 113 % |
Sample type Cell culture media | Average % = 112.7 | Range 93 - 138 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's MDC (Macrophage Derived Chemokine) Mouse ELISA Kit (ab193726) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MDC in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MDC coated onto a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures MDC present in the sample. The wells are washed and a biotinylated anti-mouse MDC antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted into the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of MDC bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MDC also known as CCL22 or macrophage-derived chemokine is a small protein involved in immune system signaling. The molecular mass of MDC is approximately 8 kDa. It is expressed in various tissues including lymph nodes thymus and lungs where it mainly acts to regulate immune cell movement. Researchers commonly study this target using MDC assays and CCL22 ELISA to better understand its functions and expression patterns.
- Biological function summary
MDC interacts with immune cells to mediate responses such as chemotaxis. It binds to the CCR4 receptor which is found on T cells and other immune cells. MDC does not require complex formation to function; it works independently to attract specific cell types. This chemokine provides a way for immune cells to navigate to sites of inflammation or infection.
- Pathways
MDC plays a significant role in inflammatory and immune response pathways. It is integrally linked with the chemokine signaling pathway where it works along with other chemokines to guide immune responses. MDC is related to proteins like CCR4 and other chemokines in these pathways facilitating communication between cells during immune responses and maintaining homeostasis.
- Associated diseases and disorders
MDC is implicated in conditions such as allergic responses and autoimmune diseases. Its interaction with CCR4 connects it to disorders like asthma and atopic dermatitis where excessive immune cell recruitment leads to inflammation. Studying MDC levels through macrophage assays and chemokine ELISAs can provide insights into its role in these diseases and help develop potential therapeutic strategies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Mouse MDC ELISA Kit (Macrophage Derived Chemokine) (ab193726)
Example of typical Mouse MDC standard curve using Assay dilutant A.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Sandwich ELISA - Mouse MDC ELISA Kit (Macrophage Derived Chemokine) (ab193726)
Example of typical Mouse MDC standard curve using Assay dilutant B.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com