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AB193726

Mouse MDC ELISA Kit (Macrophage Derived Chemokine)

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Mouse MDC ELISA Kit (Macrophage Derived Chemokine) is a Sandwich (quantitative) ELISA for the measurement of Mouse MDC (Macrophage Derived Chemokine) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Abcd1, Scya22, Ccl22, C-C motif chemokine 22, Activated B and dendritic cell-derived, CC chemokine ABCD-1, Small-inducible cytokine A22

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Sandwich ELISA - Mouse MDC ELISA Kit (Macrophage Derived Chemokine) (AB193726)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse MDC ELISA Kit (Macrophage Derived Chemokine) (AB193726)

Example of typical Mouse MDC standard curve using Assay dilutant A.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Sandwich ELISA - Mouse MDC ELISA Kit (Macrophage Derived Chemokine) (AB193726)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse MDC ELISA Kit (Macrophage Derived Chemokine) (AB193726)

Example of typical Mouse MDC standard curve using Assay dilutant B.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 2 pg/mL

Range

2 - 600 pg/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MDC (Macrophage Derived Chemokine) Mouse ELISA Kit (ab193726) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MDC in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse MDC coated onto a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures MDC present in the sample. The wells are washed and a biotinylated anti-mouse MDC antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted into the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of MDC bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "103 - 117 %", "average": "= 108.6" }, { "sample": "Plasma", "range": "94 - 113 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "93 - 138 %", "average": "= 112.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193726-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193726-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193726-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Mouse MDC detection antibody", "number":"AB193726-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193726-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193726-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated mouse MDC Microplate (12 strips x 8 wells)", "number":"AB193726-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB193726-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"mouse MDC Standards (lyophilized)", "number":"AB193726-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193726-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDC also known as CCL22 or macrophage-derived chemokine is a small protein involved in immune system signaling. The molecular mass of MDC is approximately 8 kDa. It is expressed in various tissues including lymph nodes thymus and lungs where it mainly acts to regulate immune cell movement. Researchers commonly study this target using MDC assays and CCL22 ELISA to better understand its functions and expression patterns.
Biological function summary

MDC interacts with immune cells to mediate responses such as chemotaxis. It binds to the CCR4 receptor which is found on T cells and other immune cells. MDC does not require complex formation to function; it works independently to attract specific cell types. This chemokine provides a way for immune cells to navigate to sites of inflammation or infection.

Pathways

MDC plays a significant role in inflammatory and immune response pathways. It is integrally linked with the chemokine signaling pathway where it works along with other chemokines to guide immune responses. MDC is related to proteins like CCR4 and other chemokines in these pathways facilitating communication between cells during immune responses and maintaining homeostasis.

MDC is implicated in conditions such as allergic responses and autoimmune diseases. Its interaction with CCR4 connects it to disorders like asthma and atopic dermatitis where excessive immune cell recruitment leads to inflammation. Studying MDC levels through macrophage assays and chemokine ELISAs can provide insights into its role in these diseases and help develop potential therapeutic strategies.
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Product protocols

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Target data

Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses.
See full target information Ccl22
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websiteProtocolBooklet
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