AB210572

Mouse MER ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Mouse MER ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MER with a sensitivity of 27.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Mer, Mertk, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK

4 Images

Sandwich ELISA - Mouse MER ELISA Kit (AB210572)

sELISA

Supplier Data

Sandwich ELISA - Mouse MER ELISA Kit (AB210572)

Interpolated concentrations of native MER in mouse serum and plasma samples.

The concentrations of MER were measured in duplicates, interpolated from the MER standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 20%, plasma (citrate) 20%, plasma (heparin) 20% and plasma (EDTA) 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MER concentration was determined to be 27.7 ng/mL in serum, 28.6 ng/mL in plasma (citrate), 37.1 ng/mL in plasma (heparin) and 37.8 ng/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse MER ELISA Kit (AB210572)

Interpolated concentrations of native MER in mouse pancreas extract based on a 1,000 μg/mL extract load.

The concentrations of MER were measured in duplicate and interpolated from the MER standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MER concentration was determined to be 3.64 ng/mL in pancreas extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse MER ELISA Kit (AB210572)

Example of mouse MER standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse MER ELISA Kit (AB210572)

Example of mouse MER standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 27.8 pg/mL

Range

156 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse MER ELISA Kit (ab210572) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MER protein in plasma, serum, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MER with 27.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MER is a receptor tyrosine kinase that regulates a variety of cellular processes, including cell survival, migration, differentiation and efferocytosis along with the immune response.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 105 %", "average": "= 103" }, { "sample": "Serum", "range": "102 - 117 %", "average": "= 108" }, { "sample": "Tissue Extracts", "range": "95 - 109 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "98 - 102 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "103 - 115 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "100 - 115 %", "average": "= 105" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab210572-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB210572-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB210572-CMP13", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB210572-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse MER Lyophilized Recombinant Protein", "number":"AB210572-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MER Capture Antibody", "number":"AB210572-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB210572-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB210572-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB210572-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB210572-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB210572-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB210572-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MER Detector Antibody", "number":"AB210572-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.

Biological function summary

The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.

Pathways

Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.

Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.

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Product protocols

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Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

See full target information Mertk

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 28:172-189.e7 PubMed31269438

2019

The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhou Hou,Guobao Chen,William Bracamonte-Baran,Hee Sun Choi,Nicola L Diny,Jungeun Sung,David Hughes,Taejoon Won,Megan Kay Wood,Monica V Talor,David Joel Hackam,Karin Klingel,Giovanni Davogustto,Heinrich Taegtmeyer,Isabelle Coppens,Jobert G Barin,Daniela Čiháková

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse MER ELISA Kit

Sandwich ELISA - Mouse MER ELISA Kit (AB210572)

Sandwich ELISA - Mouse MER ELISA Kit (AB210572)

Sandwich ELISA - Mouse MER ELISA Kit (AB210572)

Sandwich ELISA - Mouse MER ELISA Kit (AB210572)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise