AB204528

Mouse Mesothelin ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Mouse Mesothelin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Mesothelin with a sensitivity of 14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Mes, Mpf, Msln, Mesothelin, Pre-pro-megakaryocyte-potentiating factor

3 Images

Sandwich ELISA - Mouse Mesothelin ELISA Kit (AB204528)

sELISA

Supplier Data

Sandwich ELISA - Mouse Mesothelin ELISA Kit (AB204528)

Interpolated concentrations of Mesothelin in mouse serum and plasma samples.

The concentrations of Mesothelin were measured in duplicates, interpolated from the Mesothelin standard curve and corrected for sample dilution. Note that 1X Diluted samples were pre-diluted to 50%. The interpolated, dilution factor-corrected values are plotted in pg of Mesothelin per mL of neat sample (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse Mesothelin ELISA Kit (AB204528)

Interpolated concentrations of Mesothelin in mouse cell extract samples.

The concentrations of Mesothelin were measured in duplicates, interpolated from the Mesothelin standard curve and corrected for sample dilution. Note that 1X Diluted C2C12 cell extract samples were pre-diluted to 125 μg/mL. Note that 1X Diluted NIH/3T3 cell extract samples were pre-diluted to 250 μg/mL. The interpolated, dilution factor-corrected values are plotted in pg of Mesothelin per mg of extract (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse Mesothelin ELISA Kit (AB204528)

Example of Mesothelin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 14 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Mesothelin ELISA Kit (ab204528) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mesothelin protein in cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Mesothelin with 14 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mesothelin is expressed as a much larger, 70 kDa precursor, which is cleaved into two functional proteins, Megakaryocyte potentiating factor and Mesothelin. Mesothelin is a glycosylated cell-surface protein, anchored to the plasma membrane via its GPI anchor. Membrane-anchored forms of Mesothelin may play a role in cellular adhesion.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" } ]

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Recovery

[ { "sample": "Serum", "range": "115.6 - 122.8 %", "average": "= 119.6" }, { "sample": "EDTA Plasma", "range": "114.4 - 118.8 %", "average": "= 116.3" }, { "sample": "Citrate plasma", "range": "108.2 - 114.6 %", "average": "= 112.1" }, { "sample": "Cell culture media", "range": "85.6 - 90 %", "average": "= 88.5" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab204528-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB204528-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB204528-CMP13", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB204528-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Mesothelin Lyophilized Recombinant Protein", "number":"AB204528-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB204528-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Mesothelin Capture Antibody", "number":"AB204528-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB204528-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB204528-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB204528-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB204528-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Mesothelin Detector Antibody", "number":"AB204528-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB204528-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB204528-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) may potentiate megakaryocyte colony formation.

See full target information Msln

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Toxicology 499:153662 PubMed37923288

2023

Pleural inflammatory response, mesothelin content and DNA damage in mice at one-year after intra-pleural carbon nanotube administration.

Applications

Unspecified application

Species

Unspecified reactive species

Regitze Sølling Wils,Nicklas Raun Jacobsen,Ulla Vogel,Martin Roursgaard,Annie Jensen,Peter Møller

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Mesothelin ELISA Kit

Sandwich ELISA - Mouse Mesothelin ELISA Kit (AB204528)

Sandwich ELISA - Mouse Mesothelin ELISA Kit (AB204528)

Sandwich ELISA - Mouse Mesothelin ELISA Kit (AB204528)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Assay Kits

Secondary Antibodies

Proteins & Peptides

Product promise