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AB275107

Mouse Met ELISA Kit (c-Met)

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Mouse Met ELISA Kit (c-Met) is a single-wash 90-min Simplestep used to quantify Mouse Met (c-Met) with a sensitivity of 10 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Tyrosine-protein kinase Met, SF receptor, Met

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Sandwich ELISA - Mouse Met ELISA Kit (c-Met) (AB275107)
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Supplier Data

Sandwich ELISA - Mouse Met ELISA Kit (c-Met) (AB275107)

Example of mouse Met standard curve in Sample Diluent NS.

The Met standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Met ELISA Kit (c-Met) (AB275107)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Met ELISA Kit (c-Met) (AB275107)

Interpolated concentrations of native Met in mouse serum, plasma and B16-F10 cell culture supernatant samples.

The concentrations of Met were measured in duplicates, interpolated from the Met standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 4%, plasma (citrate) 4%, plasma (EDTA) 4%, plasma (heparin) 4% and B16-F10 cell culture supernatant 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Met concentration was determined to be 77,215 pg/mL in neat serum, 58,861 pg/mL in neat plasma (citrate), 62,541 pg/mL in neat plasma (EDTA), 75,355 pg/mL in neat plasma (heparin) and 13,898 pg/mL in neat B16-F10 supernatant.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10 pg/mL

Range

54.69 - 3500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB51067

Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (AB51067)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Met ELISA kit (ab275107) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Met protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Met with 10 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Met, also known as Hepatocyte Growth Factor Receptor (HGFR)or Proto-Oncogene c-Met (c-Met) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor ligand. Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of Met on its intracellular domain that provides docking sites for downstream signaling molecules. The recruitment of these downstream effectors by Met leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLC gamma-PKC. During embryonic development, Met signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, Met participates in wound healing as well as organ regeneration and tissue remodeling. Met promotes also differentiation and proliferation of hematopoietic cells.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 118" }, { "sample": "Serum", "range": null, "average": "= 110" }, { "sample": "EDTA Plasma", "range": null, "average": "= 114" }, { "sample": "Cell culture media", "range": null, "average": "= 84" }, { "sample": "Heparin Plasma", "range": null, "average": "= 100" }, { "sample": "Citrate plasma", "range": null, "average": "= 115" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab275107-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB275107-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB275107-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB275107-CMP10", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB275107-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB275107-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB275107-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Met (c-Met) Capture Antibody", "number":"AB275107-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Met (c-Met) Lyophilized Recombinant Protein", "number":"AB275107-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB275107-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Met (c-Met) Detector Antibody", "number":"AB275107-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts (PubMed : 30777867). In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Also promotes differentiation and proliferation of hematopoietic cells (By similarity). May regulate cortical bone osteogenesis (PubMed : 26637977).. (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.
See full target information Met
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Product promise

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