AB209885

Mouse MIF ELISA Kit

Recombinant
SimpleStep
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(4 Publications)

Mouse MIF ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MIF with a sensitivity of 28 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Macrophage migration inhibitory factor, MIF, Delayed early response protein 6, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, DER6, GIF, Mif

4 Images

Sandwich ELISA - Mouse MIF ELISA Kit (AB209885)

sELISA

Supplier Data

Sandwich ELISA - Mouse MIF ELISA Kit (AB209885)

Interpolated concentrations of native MIF in mouse serum, plasma and cell culture supernatant samples.

The concentrations of MIF were measured in duplicates, interpolated from the MIF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 0.5%, plasma (citrate) 2%, plasma (EDTA) 2%, plasma (heparin) 2%, and stimulated RAW 264.7 cell culture supernatant 0.3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIF concentration was determined to be 1.06 μg/mL in serum, 286 ng/mL in plasma (citrate), 229 ng/mL in plasma (EDTA), 293 ng/mL in plasma (heparin), and 1.75 μg/mL in LPS-stimulated RAW 264.7 cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse MIF ELISA Kit (AB209885)

Interpolated concentrations of native MIF in RAW 264.7 cell extract and mouse spleen based on 0.49 µg/mL and 3.91 µg /mL extract loads, respectively.

The concentrations of MIF were measured in duplicate and interpolated from the MIF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIF concentration was determined to be 9.8 μg/mL in RAW 264.7 cell extract and 1.1 μg/mL in mouse spleen extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse MIF ELISA Kit (AB209885)

Example of mouse MIF standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse MIF ELISA Kit (AB209885)

Example of mouse MIF standard curve in 1X Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Tissue Homogenate, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 28 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse MIF ELISA Kit (ab209885) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIF protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, tissue extracts, and tissue homogenate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MIF with 28 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Macrophage migration inhibitory factor (MIF) is a 12.37 kDa pro-inflammatory cytokine and is involved in the innate immune response to bacterial pathogens. MIF assembles into a trimer, composed of three identical subunits. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. MIF counteracts the anti-inflammatory activity of glucocorticoids and exhibits phenylpyruvate tautomerase and dopachrome tautomerase activity. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Intra", "sample": "Mouse Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13.6" } ]

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Recovery

[ { "sample": "Serum", "range": "106 - 117 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "105 - 112 %", "average": "= 109" }, { "sample": "Cell culture extracts", "range": "108 - 113 %", "average": "= 111" }, { "sample": "Tissue Extracts", "range": "102 - 118 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "105 - 117 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "112 - 113 %", "average": "= 112" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.

Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.

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Product protocols

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Target data

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (By similarity). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (By similarity). Counteracts the anti-inflammatory activity of glucocorticoids (By similarity). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed : 10933783, PubMed : 16780921, PubMed : 19188446). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed : 10933783, PubMed : 16780921, PubMed : 19188446).

See full target information Mif

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:128 PubMed38685050

2024

Blocking the MIF-CD74 axis augments radiotherapy efficacy for brain metastasis in NSCLC via synergistically promoting microglia M1 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Lichao Liu,Jian Wang,Ying Wang,Lingjuan Chen,Ling Peng,Yawen Bin,Peng Ding,Ruiguang Zhang,Fan Tong,Xiaorong Dong

Biochemistry and biophysics reports 36:101565 PubMed37965064

2023

Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yadong Wang,Zhaohui Dang,Xu Wang,Yuanyuan Chen,Peng Dong,Gang Liu,Weibin Tan,Zhong Gui,Fan Bu,Feng Lin,Chaozhao Liang

Molecular medicine reports 25: PubMed34751412

2021

Repigmentation by combined narrow‑band ultraviolet B/adipose‑derived stem cell transplantation in the mouse model: Role of Nrf2/HO‑1‑mediated Ca homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Bian,Hao Yu,Mingzhu Jin,Xinghua Gao

Neuroscience letters 739:135390 PubMed32947004

2020

Ozone influences migration and proliferation of neural stem cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Gerardo Tricarico,Jasmina Isakovic,Min Suk Song,Franco Rustichelli,Valter Travagli,Dinko Mitrecic

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse MIF ELISA Kit

Sandwich ELISA - Mouse MIF ELISA Kit (AB209885)

Sandwich ELISA - Mouse MIF ELISA Kit (AB209885)

Sandwich ELISA - Mouse MIF ELISA Kit (AB209885)

Sandwich ELISA - Mouse MIF ELISA Kit (AB209885)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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