Mouse Milk Fat Globule 1 ELISA Kit
- Recombinant
- SimpleStep
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Mouse Milk Fat Globule 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Milk Fat Globule 1 with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Lactadherin, MFGM, Milk fat globule-EGF factor 8, SED1, Sperm surface protein SP47, MFG-E8, MP47, Mfge8
- sELISA
Supplier Data
Sandwich ELISA - Mouse Milk Fat Globule 1 ELISA Kit (AB208981)
Interpolated concentrations of native Milk Fat Globule 1 in mouse lung and liver extract samples.
The concentrations of Milk Fat Globule 1 were measured in two different dilutions in duplicate and interpolated from the Milk Fat Globule 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Milk Fat Globule 1 per mg of extract (mean +/- SD, n=3).
- sELISA
Supplier Data
Sandwich ELISA - Mouse Milk Fat Globule 1 ELISA Kit (AB208981)
Interpolated concentrations of native Milk Fat Globule 1 in mouse serum and plasma samples.
The concentrations of Milk Fat Globule 1 were measured in duplicates, interpolated from the Milk Fat Globule 1 standard curves and corrected for sample dilution. 1X diluted samples are as follows : serum 15%, plasma (citrate) 20%, plasma (EDTA) 20% and plasma (heparin) 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Milk Fat Globule 1 concentration was determined to be 16.2 ng/mL in serum, 11.6 ng/mL in plasma (citrate), 9.9 ng/mL in plasma (EDTA), 12.9 ng/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Mouse Milk Fat Globule 1 ELISA Kit (AB208981)
Interpolated concentrations of native Milk Fat Globule 1 in mouse lung tissue extract sample based on a 125 μg/mL extract load and lung tissue culture (day 5) supernatant samples (40%).
The concentrations of Milk Fat Globule 1 were measured in duplicate and interpolated from the Milk Fat Globule 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Milk Fat Globule 1 concentration was determined to be 2.64 ng/mL in mouse lung tissue extract sample and 3.8 ng/mL in lung supernatant samples.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Milk Fat Globule 1 ELISA Kit (AB208981)
Example of mouse Milk Fat Globule 1 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Milk Fat Globule 1 ELISA Kit (AB208981)
Example of mouse Milk Fat Globule 1 standard curve in Sample Diluent 12BP.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Mouse Milk Fat Globule 1 ELISA Kit (AB208981)
Mouse Milk Fat Globule 1 standard curve comparison data.
Standard curve comparison between mouse Milk Fat Globule 1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 5-fold increase in sensitivity.
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Reactivity data
Product details
Mouse Milk Fat Globule 1 ELISA Kit (ab208981) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Milk Fat Globule 1 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Milk Fat Globule 1 with 3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Milk Fat Globule 1 is a secreted glycoprotein with functions in mammary gland morphogenesis, angiogenesis and tumor progression. Milk Fat Globule 1 contributes to phagocytic removal of apoptotic cells in many tissues. It is a specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Milk Fat Globule 1 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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