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AB100726

Mouse MIP-1 alpha/CCL3 ELISA Kit

1

(1 Review)

|

(2 Publications)

Mouse MIP-1 alpha/CCL3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse MIP-1 alpha/CCL3 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Mip1a, Scya3, Ccl3, C-C motif chemokine 3, Heparin-binding chemotaxis protein, L2G25B, Macrophage inflammatory protein 1-alpha, SIS-alpha, Small-inducible cytokine A3, TY-5, MIP-1-alpha

1 Images
Sandwich ELISA - Mouse MIP-1 alpha/CCL3 ELISA Kit (AB100726)
  • sELISA

Unknown

Sandwich ELISA - Mouse MIP-1 alpha/CCL3 ELISA Kit (AB100726)

Representative standard curve using ab100726

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 8 pg/mL

Range

4.1 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MIP-1a Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-1a in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for mouse MIP-1a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP-1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 104 %", "average": "= 94.58" }, { "sample": "Serum", "range": "83 - 99 %", "average": "= 88.58" }, { "sample": "Plasma", "range": "82 - 98 %", "average": "= 87.94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100726-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100726-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100726-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse MIP-1 alpha/CCL3", "number":"AB100726-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"240X HRP-Streptavidin Concentrate", "number":"AB100726-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100726-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100726-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MIP-1 alpha/CCL3 Microplate (12 x 8 wells)", "number":"AB100726-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse MIP-1 alpha/CCL3 Standard (lyophilized)", "number":"AB100726-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 is a chemokine that plays a critical role in the immune system. With a molecular weight of around 8 kDa it is primarily expressed in activated T-cells macrophages and fibroblasts. MIP-1 alpha acts as a signaling protein that binds to receptors on target cells directing the movement of immune cells. Researchers commonly refer to it using various alternative names such as MIP-1a and CCL3-alpha rat and it is frequently measured using assays like MIP-1 alpha ELISA for experimental analyses.
Biological function summary

Proteins including MIP-1 alpha participate in the recruitment and activation of diverse immune cells aiding the inflammatory response. Through interaction with receptors like CCR1 and CCR5 MIP-1 alpha mobilizes cells like natural killer cells and monocytes. This chemokine does not generally form part of a larger protein complex but interacts directly with membrane receptors to exert its function in immune cell chemotaxis.

Pathways

MIP-1 alpha operates in the context of both the inflammatory response and the chemokine signaling pathways. Its involvement in these pathways helps mediate the trafficking of leukocytes. Notably MIP-1 alpha interacts with proteins such as CCR1 and CCR5 to transmit signals that instigate immune responses. These proteins are integral to its role in modulating immunity providing targets for research into controlling inflammatory processes.

MIP-1 alpha closely relates to conditions such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis its overexpression can lead to excessive inflammation and joint destruction. Meanwhile in HIV infection MIP-1 alpha interacts with CCR5 a co-receptor necessary for the virus entry into cells. This dual relationship elucidates its potential as a therapeutic target in managing both inflammatory diseases and viral entry mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
See full target information Ccl3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 36:101565 PubMed37965064

2023

Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yadong Wang,Zhaohui Dang,Xu Wang,Yuanyuan Chen,Peng Dong,Gang Liu,Weibin Tan,Zhong Gui,Fan Bu,Feng Lin,Chaozhao Liang

Journal of neuroinflammation 8:173 PubMed22152278

2011

Adaptive Müller cell responses to microglial activation mediate neuroprotection and coordinate inflammation in the retina.

Applications

Unspecified application

Species

Unspecified reactive species

Minhua Wang,Wenxin Ma,Lian Zhao,Robert N Fariss,Wai T Wong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB200017

Mouse MIP-1 alpha/CCL3 ELISA Kit

elisa-kits

mouse-mip-1-alpha-ccl3-elisa-kit-ab200017

3

(1 reviews)

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