Mouse MIP-1 alpha/CCL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MIP-1 alpha/CCL3 with a sensitivity of 1.95 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
View Alternative Names
Mip1a, Scya3, Ccl3, C-C motif chemokine 3, Heparin-binding chemotaxis protein, L2G25B, Macrophage inflammatory protein 1-alpha, SIS-alpha, Small-inducible cytokine A3, TY-5, MIP-1-alpha
- sELISA
Supplier Data
Sandwich ELISA - Mouse MIP-1 alpha/CCL3 ELISA Kit (AB200017)
Comparison of secreted MIP1a in unstimulated and LPS-stimulated RAW264.7 cells.
RAW264.7 cells were grown in the absence (unstimulated) or presence of LPS (stimulated) for 2 days. MIP1a was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the MIP1a standard curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 20 ng/mL unstimulated, 118 ng/mL stimulated, and undetectable in media.
- sELISA
Supplier Data
Sandwich ELISA - Mouse MIP-1 alpha/CCL3 ELISA Kit (AB200017)
Example of MIP1a standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse MIP-1 alpha/CCL3 ELISA Kit (AB200017)
Interpolated concentrations of MIP1a in RAW264.7 LPS Stimulated supernatant.
The concentrations of MIP1a were measured in duplicate and interpolated from the MIP1a standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1a concentration was determined to be 118 μg/mL in RAW264.7 LPS Stimulated supernatant.
Complementary Products
Reactivity data
Product details
Mouse MIP1a ELISA Kit (CCL3 ) (ab200017) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP1a (CCL3) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MIP1a (CCL3) with 1.95 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Macrophage Inflammatory Protein 1 alpha (MIP1a, also known as C-C motif chemokine 3 or CCL3) is a secreted monokine with inflammatory and chemokinetic properties. Mature MIP1a is 68 amino acids after cleavage of a 23 amino acid signal peptide. MIP1a can be induced in a variety of cell types and for immune and inflammatory response. In humans, MIP1a is a major HIV-suppressive factor produced by CD8+ T cells. The standard protein in this kit is mature mouse MIP1a.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Proteins including MIP-1 alpha participate in the recruitment and activation of diverse immune cells aiding the inflammatory response. Through interaction with receptors like CCR1 and CCR5 MIP-1 alpha mobilizes cells like natural killer cells and monocytes. This chemokine does not generally form part of a larger protein complex but interacts directly with membrane receptors to exert its function in immune cell chemotaxis.
Pathways
MIP-1 alpha operates in the context of both the inflammatory response and the chemokine signaling pathways. Its involvement in these pathways helps mediate the trafficking of leukocytes. Notably MIP-1 alpha interacts with proteins such as CCR1 and CCR5 to transmit signals that instigate immune responses. These proteins are integral to its role in modulating immunity providing targets for research into controlling inflammatory processes.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (3)
Recent publications for all applications. Explore the full list and refine your search
Biology 9: PubMed32938018
2020
Applications
Unspecified application
Species
Unspecified reactive species
Cells 9: PubMed32660087
2020
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 8:2226 PubMed29263385
2017
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com