Mouse MIP-1 gamma ELISA Kit (CCL9)
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(2 Publications )
- sELISA
Unknown
Sandwich ELISA - Mouse MIP-1 gamma ELISA Kit (CCL9) (AB155449)
Representative standard curve using ab155449 - Assay Diluent B.
- sELISA
Unknown
Sandwich ELISA - Mouse MIP-1 gamma ELISA Kit (CCL9) (AB155449)
Representative standard curve using ab155449 - Assay Diluent A.
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Reactivity data
Product details
Abcam's MIP-1 (CCL9) gamma Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse MIP-1 gamma in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP-1 (CCL9 gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP-1 gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Precision & recovery information
Precision
[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
Recovery
[ { "sample": "Cell culture media", "range": "95 - 103 %", "average": "= 99.02" }, { "sample": "Plasma", "range": "81 - 101 %", "average": "= 90.1" }, { "sample": "Serum", "range": "75 - 91 %", "average": "= 83.5" } ]
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MIP-1γ contributes to immune response modulation by acting as a signaling molecule. It helps coordinate the movement and activation of immune cells such as monocytes and lymphocytes thereby modulating inflammation. Unlike some other chemokines MIP-1γ does not usually form a complex with proteins. Its primary role involves direct interactions with specific receptors on target cells that initiate intracellular signaling pathways leading to cell migration and activation.
Pathways
MIP-1γ engages in chemokine signaling and inflammatory response pathways. It intricately associates with the chemokine receptor CCR1 which is vital for mediating signal transduction processes essential for cell movement and immune surveillance. MIP-1γ also plays a role in the NF-kB signaling pathway important for transcription regulation and inflammatory response. This relationship highlights its involvement in orchestrating immune cell migration and the regulation of immune responses.
Product protocols
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Target data
Alternative Names
Publications (2)
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Viruses 15: PubMed37515181
2023
Applications
Unspecified application
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Unspecified reactive species
Journal of immunology research 2022:7200379 PubMed35465352
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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