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AB100727

Mouse MIP2 ELISA Kit (CXCL2)

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(1 Publication)

Mouse MIP2 ELISA Kit (CXCL2) is a Sandwich (quantitative) ELISA for the measurement of Mouse MIP2 (CXCL2) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2

2 Images
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB100727)
  • sELISA

Unknown

Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB100727)

Representative standard curve using ab100727

Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB100727)
  • sELISA

Unknown

Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB100727)

Representative standard curve using ab100727

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 8 pg/mL

Range

4.1 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MIP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP2 in plasma and cell culture supernatants. (Mouse MIP2 concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for mouse MIP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Mouse MIP2 in normal serum with ab100727, only in serum spiked with Mouse MIP2.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 104 %", "average": "= 94.58" }, { "sample": "Plasma", "range": "82 - 98 %", "average": "= 87.94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100727-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100727-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100727-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB100727-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100727-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse MIP2", "number":"AB100727-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse MIP2 Standard (lyophilized)", "number":"AB100727-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100727-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100727-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MIP2 Microplate (12 x 8 wells)", "number":"AB100727-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CXCL2 protein also known as macrophage inflammatory protein 2 (MIP-2) or KC in the mouse model functions as a chemokine. It weighs approximately 7.8 kDa and is part of the CXC chemokine family. CXCL2 is mainly expressed in macrophages neutrophils and certain epithelial cells. It plays an important role in mediating the migration of these cells to sites of inflammation or injury.
Biological function summary

CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.

Pathways

CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).

CXCL2 is connected to inflammatory conditions and diseases like sepsis and inflammatory bowel disease (IBD). Elevated levels of CXCL2 can enhance the inflammatory response which is linked to the progression of these conditions. The protein is also associated with tumor necrosis factor-alpha (TNF-alpha) which amplifies inflammation and tissue damage during disease processes.
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Product protocols

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Target data

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.
See full target information Cxcl2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 15:4710-4727 PubMed33626869

2021

Quantum Dot Nanomedicine Formulations Dramatically Improve Pharmacological Properties and Alter Uptake Pathways of Metformin and Nicotinamide Mononucleotide in Aging Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas J Hunt,Glen P Lockwood,Sun W S Kang,Lara J Westwood,Christina Limantoro,Wojciech Chrzanowski,Peter A G McCourt,Zdenka Kuncic,David G Le Couteur,Victoria C Cogger
View all publications
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websiteProtocolBooklet
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