Mouse MIP2 ELISA Kit (CXCL2) is a single-wash 90-min Simplestep used to quantify Mouse MIP2 (CXCL2) with a sensitivity of 0.87 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
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- Cited in over 5 citations
View Alternative Names
Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2
- ELISA
Supplier Data
ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB204517)
- sELISA
Supplier Data
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB204517)
Linearity of dilution of MIP2 in serum and cell culture media.
Recombinant mouse MIP2 was spiked into 100% serum and diluted in a 2-fold dilution series in Sample Diluent 50BP. Recombinant mouse MIP2 was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse MIP2 were measured in duplicate and interpolated from the mouse MIP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
- sELISA
Supplier Data
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB204517)
Mouse MIP2 expression is shown for serum and plasma samples.
Native MIP2 was measured in duplicate in 100% serum and 100% heparin plasma and concentrations interpolated from a standard curve diluted in Sample Diluent 50BP. Native MIP2 was measured in duplicate in 100% Citrate plasma and 50% EDTA plasma and concentrations interpolated from a standard curve diluted in Sample Diluent 25BP. The interpolated dilution factor corrected values are graphed (mean +/- SD).
- sELISA
Supplier Data
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB204517)
Linearity of dilution of spiked recombinant MIP2 in plasma.
Recombinant mouse MIP2 was spiked into 100% citrate plasma and 50% EDTA plasma and diluted in a 2-fold dilution series in Sample Diluent 25BP. Recombinant mouse MIP2 was spiked into 100% heparin plasma and diluted in a 2-fold dilution series in Sample Diluent 50BP. The concentrations of mouse MIP2 were measured in duplicate and interpolated from the mouse MIP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
- sELISA
Unknown
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB204517)
Mouse MIP2 standard curve comparison data.
Standard curve comparison between mouse MIP2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit show comparable sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB204517)
Mouse MIP2 expression is shown for cultured media from two mouse cell lines.
J774A.1 cells were cultured in HGDMEM with 10% fetal calf serum, and 100 μg/mL of Kanamycin. During the exponential growth phase, J774A.1 cells were treated for 72 hours in the presence and absence of 1.5% PHA and 10 ng/mL of PMA. RAW 264.7 cells were cultured in HGDMEM with 10% fetal calf serum, 2 mM L-glutamine and 100 μg/mL Kanamycin. During the exponential growth phase, RAW264.7 cells were starved for 24 hours and treated in the presence and absence of 5 μg/mL of LPS (Batch #1) or 1% PHA (Batch #2).The concentrations of mouse MIP2 were interpolated from a standard curve diluted in Sample Diluent NS and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
- sELISA
Supplier Data
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (AB204517)
Example of the mouse MIP2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Mouse MIP2 ELISA Kit (CXCL2) ab204517 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse MIP2 (CXCL2)in cell culture supernatant, plasma, serum. This SimpleStep sensitivity is 0.87 pg/mL.
How the assay works
Mouse MIP2 (CXCL2)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse MIP2 ELISA Kit (CXCL2) ab204517 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Mouse Macrophage Inflammatory Protein-2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 was originally identified as a heparin-binding protein, and has been shown to exhibit potent neutrophil chemotactic activity. Mouse MIP2 c-DNA encodes a 100 amino acid residue precursor protein. The amino-terminal 27 amino acid residues are cleaved from this precursor to generate the mature mouse MIP2. Mouse MIP2 is 63% identical to Mouse KC (another mouse alpha chemokine), and mouse MIP2 is 60% identical to human GRO? and GRO?. Based on these protein sequence similarities, it is likely that mouse MIP2 and KC are homologs of the human GRO?, ?, and ? chemokines. However, since chemokines with protein sequence homology to human IL-8 have not been identified in mice, it has been suggested that the mouse MIP2 and KC are functional homologs of human IL-8 in mice. A putative mouse homolog of the human IL-8 receptor beta (IL-8 R?) has also been cloned. This receptor shows 71% identity to human IL-8 R? and 68% identity to human IL-8 R?. Both mouse MIP2 and KC bind mouse IL-8 R? with high affinity.
Precision
Recovery
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Properties and storage information
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Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.
Pathways
CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).
Product protocols
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Publications (6)
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Cell death & disease 14:393 PubMed37391422
2023
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Cell death & disease 13:562 PubMed35732633
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Cytotechnology 73:669-682 PubMed34349355
2021
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Cells 9: PubMed32660087
2020
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British journal of cancer 123:459-470 PubMed32439934
2020
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Journal of Crohn's & colitis : PubMed32333776
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