Mouse MIP2 ELISA Kit (CXCL2) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse MIP2 (CXCL2) in Plasma, Cell culture supernatant, Serum samples.
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- Cell death & disease 14:3932023KLF6 alleviates hepatic ischemia-reperfusion injury by inhibiting autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiye Li,Dongsheng Yu,Chenhui He,Qiwen Yu,Zhongkun Huo,Yi Zhang,Shuijun ZhangPubMed 37391422
- Cell death & disease 13:5622022LncRNA IRAR regulates chemokines production in tubular epithelial cells thus promoting kidney ischemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Jia,Sujuan Xu,Ting Ren,Tianyi Pan,Xiaoyan Wang,Yunlu Zhang,Zhouping Zou,Man Guo,Qi Zeng,Bo Shen,Xiaoqiang DingPubMed 35732633
- Cytotechnology 73:669-6822021Effect of short-time treatment with TNF-α on stem cell activity and barrier function in enteroids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuki Saito,Makoto Shimizu,Ken Iwatsuki,Hikaru Hanyu,Miki Tadaishi,Yoshiko Sugita-Konishi,Kazuo Kobayashi-HattoriPubMed 34349355
- Cells 9:2020The Role of Alveolar Macrophages in the Improved Protection against Respiratory Syncytial Virus and Pneumococcal Superinfection Induced by the Peptidoglycan of CRL1505.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatricia Clua et. al.PubMed 32660087
- British journal of cancer 123:459-4702020Omental adipocytes promote peritoneal metastasis of gastric cancer through the CXCL2-VEGFA axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMakoto Natsume et. al.PubMed 32439934
- Journal of Crohn's & colitis :2020Mincle/Syk signaling promotes intestinal mucosal inflammation through induction of macrophage pyroptosis in Crohn's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenbin Gong et. al.PubMed 32333776
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 3.13 - 200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.87 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.
Alternative names
Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2
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Mouse MIP2 ELISA Kit (CXCL2) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse MIP2 (CXCL2) in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 3.13 - 200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.87 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 8 | mean - | SD - | C.V. 1.64 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 4.87 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 108 | Range 103 - 113 % |
Sample type Cell culture media | Average % = 102 | Range 98 - 107 % |
Sample type Citrate plasma | Average % = 91 | Range 87 - 97 % |
Sample type EDTA Plasma | Average % = 115 | Range 113 - 116 % |
Sample type Heparin Plasma | Average % = 104 | Range 101 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse MIP2 ELISA Kit (CXCL2) (ab204517) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP2 (CXCL2) protein in cell culture supernatant, plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MIP2 (CXCL2) with 0.87 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse Macrophage Inflammatory Protein-2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 was originally identified as a heparin-binding protein, and has been shown to exhibit potent neutrophil chemotactic activity. Mouse MIP2 c-DNA encodes a 100 amino acid residue precursor protein. The amino-terminal 27 amino acid residues are cleaved from this precursor to generate the mature mouse MIP2. Mouse MIP2 is 63% identical to Mouse KC (another mouse alpha chemokine), and mouse MIP2 is 60% identical to human GROβ and GROγ. Based on these protein sequence similarities, it is likely that mouse MIP2 and KC are homologs of the human GROα, β, and γ chemokines. However, since chemokines with protein sequence homology to human IL-8 have not been identified in mice, it has been suggested that the mouse MIP2 and KC are functional homologs of human IL-8 in mice. A putative mouse homolog of the human IL-8 receptor beta (IL-8 Rβ) has also been cloned. This receptor shows 71% identity to human IL-8 Rβ and 68% identity to human IL-8 Rα. Both mouse MIP2 and KC bind mouse IL-8 Rβ with high affinity.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CXCL2 protein also known as macrophage inflammatory protein 2 (MIP-2) or KC in the mouse model functions as a chemokine. It weighs approximately 7.8 kDa and is part of the CXC chemokine family. CXCL2 is mainly expressed in macrophages neutrophils and certain epithelial cells. It plays an important role in mediating the migration of these cells to sites of inflammation or injury.
- Biological function summary
CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.
- Pathways
CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).
- Associated diseases and disorders
CXCL2 is connected to inflammatory conditions and diseases like sepsis and inflammatory bowel disease (IBD). Elevated levels of CXCL2 can enhance the inflammatory response which is linked to the progression of these conditions. The protein is also associated with tumor necrosis factor-alpha (TNF-alpha) which amplifies inflammation and tissue damage during disease processes.
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6 product images
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (ab204517)
Mouse MIP2 standard curve comparison data.
Standard curve comparison between mouse MIP2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit show comparable sensitivity.
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (ab204517)
Linearity of dilution of MIP2 in serum and cell culture media.
Recombinant mouse MIP2 was spiked into 100% serum and diluted in a 2-fold dilution series in Sample Diluent 50BP. Recombinant mouse MIP2 was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse MIP2 were measured in duplicate and interpolated from the mouse MIP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (ab204517)
Linearity of dilution of spiked recombinant MIP2 in plasma.
Recombinant mouse MIP2 was spiked into 100% citrate plasma and 50% EDTA plasma and diluted in a 2-fold dilution series in Sample Diluent 25BP. Recombinant mouse MIP2 was spiked into 100% heparin plasma and diluted in a 2-fold dilution series in Sample Diluent 50BP. The concentrations of mouse MIP2 were measured in duplicate and interpolated from the mouse MIP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (ab204517)
Mouse MIP2 expression is shown for serum and plasma samples.
Native MIP2 was measured in duplicate in 100% serum and 100% heparin plasma and concentrations interpolated from a standard curve diluted in Sample Diluent 50BP. Native MIP2 was measured in duplicate in 100% Citrate plasma and 50% EDTA plasma and concentrations interpolated from a standard curve diluted in Sample Diluent 25BP. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (ab204517)
Mouse MIP2 expression is shown for cultured media from two mouse cell lines.
J774A.1 cells were cultured in HGDMEM with 10% fetal calf serum, and 100 μg/mL of Kanamycin. During the exponential growth phase, J774A.1 cells were treated for 72 hours in the presence and absence of 1.5% PHA and 10 ng/mL of PMA. RAW 264.7 cells were cultured in HGDMEM with 10% fetal calf serum, 2 mM L-glutamine and 100 μg/mL Kanamycin. During the exponential growth phase, RAW264.7 cells were starved for 24 hours and treated in the presence and absence of 5 μg/mL of LPS (Batch #1) or 1% PHA (Batch #2).The concentrations of mouse MIP2 were interpolated from a standard curve diluted in Sample Diluent NS and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (ab204517)
Example of the mouse MIP2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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