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AB100729

Mouse MIP3 beta ELISA Kit (CCL19)

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(2 Publications)

Mouse MIP3 beta ELISA Kit (CCL19) is a Sandwich (quantitative) ELISA for the measurement of Mouse MIP3 beta (CCL19) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Elc, Scya19, Ccl19, C-C motif chemokine 19, Epstein-Barr virus-induced molecule 1 ligand chemokine, Small-inducible cytokine A19, EBI1 ligand chemokine, ELC

1 Images
Sandwich ELISA - Mouse MIP3 beta ELISA Kit (CCL19) (AB100729)
  • sELISA

Unknown

Sandwich ELISA - Mouse MIP3 beta ELISA Kit (CCL19) (AB100729)

Representative standard curve using ab100729

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

4.1 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MIP3 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-3- beta in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse MIP3 beta coated on a 96- well plate. Standards and samples are pipetted into the wells and MIP3 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "78 - 101 %", "average": "= 85.6" }, { "sample": "Serum", "range": "82 - 132 %", "average": "= 107.7" }, { "sample": "Plasma", "range": "83 - 140 %", "average": "= 119.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100729-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100729-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100729-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100729-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MIP3 beta Microplate (12 x 8 wells)", "number":"AB100729-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse MIP3 beta", "number":"AB100729-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse MIP3 beta Standard (lyophilized)", "number":"AB100729-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100729-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"260X HRP-Streptavidin Concentrate", "number":"AB100729-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-3 beta also known as CCL19 is a chemokine involved in immune responses. It weighs around 11 kDa and is encoded by the CCL19 gene. MIP-3 beta does its function by binding to its receptor CCR7 which is common in lymph nodes and other lymphoid tissues. It recruits and directs the migration of immune cells especially T cells and dendritic cells navigating them towards lymphoid tissues. This makes MIP-3 beta integral in initiating and modulating immune responses.
Biological function summary

MIP-3 beta helps direct cell trafficking within the lymphoid organs. It is not part of a complex but its interaction with the CCR7 receptor is essential for its biological activity. This binding plays a role in the development of secondary lymphoid organs and immune surveillance. Furthermore MIP-3 beta supports the homing of immune cells to appropriate locations impacting both innate and adaptive immune responses.

Pathways

MIP-3 beta is associated with the chemokine signaling pathway and the immune system pathway. Through these pathways it interacts with proteins like CCR7 and other chemokines that regulate leukocyte migration and activation. These pathways ensure local and systemic immune responses are efficiently orchestrated impacting inflammation and immune regulation.

MIP-3 beta has connections to autoimmune diseases like rheumatoid arthritis and cancer. Its deregulation can result in abnormal immune surveillance contributing to the pathology of these conditions. MIP-3 beta regulates immune cell infiltration and any imbalance may also link it to other inflammatory disorders. Relations to proteins such as CCR7 in these conditions emphasize its role in disease pathogenesis and highlight its potential as a therapeutic target.
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Product protocols

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Target data

Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
See full target information Ccl19
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 8: PubMed32675195

2020

Local injection of CCL19-expressing mesenchymal stem cells augments the therapeutic efficacy of anti-PD-L1 antibody by promoting infiltration of immune cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichi Iida,Rintaro Yoshikawa,Akihiko Murata,Hitoshi Kotani,Yasuhiro Kazuki,Mitsuo Oshimura,Yumi Matsuzaki,Mamoru Harada

Cancer discovery 8:196-215 PubMed29101162

2017

Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids.

Applications

Unspecified application

Species

Unspecified reactive species

Russell W Jenkins,Amir R Aref,Patrick H Lizotte,Elena Ivanova,Susanna Stinson,Chensheng W Zhou,Michaela Bowden,Jiehui Deng,Hongye Liu,Diana Miao,Meng Xiao He,William Walker,Gao Zhang,Tian Tian,Chaoran Cheng,Zhi Wei,Sangeetha Palakurthi,Mark Bittinger,Hans Vitzthum,Jong Wook Kim,Ashley Merlino,Max Quinn,Chandrasekar Venkataramani,Joshua A Kaplan,Andrew Portell,Prafulla C Gokhale,Bart Phillips,Alicia Smart,Asaf Rotem,Robert E Jones,Lauren Keogh,Maria Anguiano,Lance Stapleton,Zhiheng Jia,Michal Barzily-Rokni,Israel Cañadas,Tran C Thai,Marc R Hammond,Raven Vlahos,Eric S Wang,Hua Zhang,Shuai Li,Glenn J Hanna,Wei Huang,Mai P Hoang,Adriano Piris,Jean-Pierre Eliane,Anat O Stemmer-Rachamimov,Lisa Cameron,Mei-Ju Su,Parin Shah,Benjamin Izar,Manisha Thakuria,Nicole R LeBoeuf,Guilherme Rabinowits,Viswanath Gunda,Sareh Parangi,James M Cleary,Brian C Miller,Shunsuke Kitajima,Rohit Thummalapalli,Benchun Miao,Thanh U Barbie,Vivek Sivathanu,Joshua Wong,William G Richards,Raphael Bueno,Charles H Yoon,Juan Miret,Meenhard Herlyn,Levi A Garraway,Eliezer M Van Allen,Gordon J Freeman,Paul T Kirschmeier,Jochen H Lorch,Patrick A Ott,F Stephen Hodi,Keith T Flaherty,Roger D Kamm,Genevieve M Boland,Kwok-Kin Wong,David Dornan,Cloud Peter Paweletz,David A Barbie
View all publications
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websiteProtocolBooklet
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