Mouse MMP-12 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse MMP-12 in Mouse in Cell culture supernatant, Heparin Plasma, Serum samples.
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- The Journal of clinical investigation 134:2023Itaconate suppresses atherosclerosis by activating a Nrf2-dependent antiinflammatory response in macrophages in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianrui Song,Yanling Zhang,Ryan A Frieler,Anthony Andren,Sherri Wood,Daniel J Tyrrell,Peter Sajjakulnukit,Jane C Deng,Costas A Lyssiotis,Richard M Mortensen,Morgan Salmon,Daniel R GoldsteinPubMed 38085578
- Stem cell reviews and reports 18:2164-21782022The miR151 and miR5100 Transfected Bone Marrow Stromal Cells Increase Myoblast Fusion in IGFBP2 Dependent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBartosz Mierzejewski,Zuzanna Michalska,Daniel Jackowski,Władysława Streminska,Katarzyna Janczyk-Ilach,Marta Koblowska,Roksana Iwanicka-Nowicka,Agnieszka Gromadka,Maria Anna Ciemerych,Edyta BrzoskaPubMed 35190967
- Nature communications 13:3182022Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Spix,Elisabeth S Butz,Cheng-Chang Chen,Anna Scotto Rosato,Rachel Tang,Aicha Jeridi,Veronika Kudrina,Eva Plesch,Philipp Wartenberg,Elisabeth Arlt,Daria Briukhovetska,Meshal Ansari,Gizem Günes Günsel,Thomas M Conlon,Amanda Wyatt,Sandra Wetzel,Daniel Teupser,Lesca M Holdt,Fabien Ectors,Ingrid Boekhoff,Ulrich Boehm,Jaime García-Añoveros,Paul Saftig,Martin Giera,Sebastian Kobold,Herbert B Schiller,Susanna Zierler,Thomas Gudermann,Christian Wahl-Schott,Franz Bracher,Ali Önder Yildirim,Martin Biel,Christian GrimmPubMed 35031603
- iScience 24:1032012021RSV infection-elicited high MMP-12-producing macrophages exacerbate allergic airway inflammation with neutrophil infiltration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAiri Makino,Takehiko Shibata,Mashiro Nagayasu,Ikuo Hosoya,Toshiyo Nishimura,Chihiro Nakano,Kisaburo Nagata,Toshihiro Ito,Yoshimasa Takahashi,Shigeki NakamuraPubMed 34703996
- Communications biology 2:3072019Targeting p16-induced senescence prevents cigarette smoke-induced emphysema by promoting IGF1/Akt1 signaling in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher T Cottage et. al.PubMed 31428695
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 3h 30m
- Sensitivity
- < 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity).
Alternative names
Mme, Mmel, Mmp12, Macrophage metalloelastase, MME, Matrix metalloproteinase-12, MMP-12
What's included?
Recommended products
Mouse MMP-12 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse MMP-12 in Mouse in Cell culture supernatant, Heparin Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 3h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- < 10 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 16 | mean 337 pg/mL | SD 18.2 | C.V. 5.4 |
Sample Sample 2 | n 16 | mean 1472 pg/mL | SD 92.74 | C.V. 6.3 |
Sample Sample 3 | n 16 | mean 2522 pg/mL | SD 121.1 | C.V. 4.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 24 | mean 426 pg/mL | SD 27.69 | C.V. 6.5 |
Sample Sample 2 | n 24 | mean 1538 pg/mL | SD 109.2 | C.V. 7.1 |
Sample Sample 3 | n 24 | mean 2768 pg/mL | SD 157.8 | C.V. 5.7 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
The Mouse MMP-12 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213878) is designed for the quantitative detection of Mouse MMP-12 in cell culture supernatants, serum and plasma (heparin).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polycloncal antibody from goat specific for MMP-12 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A18-C462) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MMP-12 amount of sample captured in plate.
Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may be involved in tissue injury and remodeling.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Matrix metallopeptidase 12 commonly known as MMP12 or macrophage elastase is an enzyme with a molecular mass of approximately 54 kDa. Found mainly in macrophages MMP12 belongs to the matrix metallopeptidase family. It functions to break down extracellular matrix proteins specifically elastin. Besides macrophages it sees expression in other tissues including the lungs and skin but it holds particular importance in immune cell-related environments.
- Biological function summary
MMP12 plays an important role in tissue remodeling and repair processes. It interacts with other proteases within the extracellular matrix. The degradation of elastin by MMP12 contributes to structural organization and it participates in immune response modulation. As part of the matrix metalloproteinase family MMP12 takes part in the larger proteolytic system which comprises multiple enzymes working in concert.
- Pathways
MMP12 is an essential factor in the extracellular matrix degradation pathway. This pathway regulates tissue remodeling and inflammatory response. MMP12 independently breaks down elastin while it interacts with proteins like MMP2 and MMP9 which assist in remodeling processes. It also contributes to the regulation of cytokines that orchestrate immune functions.
- Associated diseases and disorders
Overexpression of MMP12 is strongly linked to chronic obstructive pulmonary disease (COPD) and atherosclerosis. In COPD MMP12's breakdown of elastin contributes to tissue damage in the lung. In atherosclerosis the enzyme accelerates plaque destabilization impacting cardiovascular health. Additionally MMP12 shares functional relationships with proteins such as TIMPs (tissue inhibitors of metalloproteinases) which regulate its activity and play roles in disease pathogenesis.
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Sandwich ELISA - Mouse MMP-12 ELISA Kit (ab213878)
Mouse MMP-12 ELISA Kit (ab213878) Standard Curve
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