Mouse MMP-2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse MMP-2 in Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- TH open : companion journal to thrombosis and haemostasis 7:e1-e132023Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeiichi Hiramoto,Nobuyuki Akita,Junji Nishioka,Koji SuzukiPubMed 36751299
- Folia neuropathologica 59:359-3712022USP38 might be a new therapeutic target for glioma via regulation of cancer cell metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Hu,Ke-Ni Wang,Hai-Rui LingHuPubMed 35114776
- Autophagy 17:4062-40822021Combination of autophagy and NFE2L2/NRF2 activation as a treatment approach for neuropathic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Li,Mouli Tian,Tong Hua,Haowei Wang,Mei Yang,Wenqian Li,Xiaoping Zhang,Hongbin YuanPubMed 33834930
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse, Cow
- Range
109.38 - 7000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 26.91 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity).PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels (By similarity).Isoform 2Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Alternative names
72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, Mmp2
What's included?
Recommended products
Mouse MMP-2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse MMP-2 in Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse, Cow
- Range
109.38 - 7000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 26.91 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 95 | Range 87 - 108 % |
Sample type Serum | Average % = 95 | Range 83 - 106 % |
Sample type EDTA Plasma | Average % = 95 | Range 80 - 118 % |
Sample type Heparin Plasma | Average % = 95 | Range 85 - 113 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse MMP-2 ELISA Kit (ab254516) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP-2 protein in cell culture supernatant, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MMP-2 with 26.91 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse MMP-2 (matrix metalloproteinase 2) is a 72 kD protein that is a part of the matrix metalloproteinases (MMPs) family consisting of 24 known human zinc proteases. MMPs essential role is breaking down components of the extracellular matrix. Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell adhesion molecules, and other proteinases. Most MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. MMPs are synthesized as pro-enzymes and secreted before conversion to their active form. MMP activity is regulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases. Mouse MMP-2 shares 96%, 99%, 95%, and 96% sequence homology with human, rat, cow, and monkey respectively.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MMP-2 protein also known as matrix metalloproteinase-2 or gelatinase A is an enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling and cell migration. Comprised of a molecular weight of approximately 72 kDa this metalloproteinase is secreted as an inactive proenzyme that requires activation. MMP2 is expressed in various tissues including the brain heart and blood vessels where it contributes to normal physiological processes and pathological conditions.
- Biological function summary
Matrix metalloproteinase-2 is mainly involved in the degradation of type IV and V collagens gelatin and fibronectin. As part of the metalloproteinase family it works alongside other MMPs to maintain tissue homeostasis and repair. MMP-2 forms part of a complex network that ensures the timely degradation of matrix components balancing synthesis and breakdown. It remains regulated by tissue inhibitors of metalloproteinases (TIMPs) preventing excessive degradation that could lead to tissue damage.
- Pathways
MMP-2 plays a significant role within the extracellular matrix (ECM) remodeling and angiogenesis pathways. It interacts with various proteins including integrins and TIMP-2 to modulate cellular behaviors such as migration and invasion. MMP-2 contributes to processes like wound healing and embryonic development through its involvement in ECM degradation and new tissue formation.
- Associated diseases and disorders
Matrix metalloproteinase-2 is linked to cancer progression and cardiovascular diseases. In cancer abnormal MMP-2 activity facilitates tumor invasion and metastasis by breaking down matrix barriers. Increased MMP-2 expression associates with poor prognosis in cancers like breast and prostate. In cardiovascular diseases such as atherosclerosis it contributes to plaque destabilization and vascular remodeling. The imbalance in MMP-2 activity and its regulation by proteins like TIMP-1 are involved in the pathology of these disorders.
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3 product images
Sandwich ELISA - Mouse MMP-2 ELISA Kit (ab254516)
Mouse MMP-2 standard curve comparison.
Standard Curve comparison between Mouse MMP-2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Mouse MMP-2 ELISA Kit (ab254516)
Example of mouse MMP-2 standard curve in Sample Diluent NS.
The MMP-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse MMP-2 ELISA Kit (ab254516)
Interpolated concentrations of native MMP-2 in mouse serum and plasma samples.
The concentrations of MMP-2 were measured in duplicates, interpolated from the MMP-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.3%, plasma (EDTA) 0.3% and plasma (heparin) 0.3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-2 concentration was determined to be 824.05 ng/mL in serum, 737.92 ng/mL in plasma (EDTA), 884.77 ng/mL in plasma (citrate).
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