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AB100731

Mouse MMP3 ELISA Kit

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(2 Publications)

Mouse MMP3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse MMP3 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Stromelysin-1, SL-1, EMS-2, Matrix metalloproteinase-3, Transin-1, MMP-3, Mmp3

1 Images
Sandwich ELISA - Mouse MMP3 ELISA Kit (AB100731)
  • sELISA

Unknown

Sandwich ELISA - Mouse MMP3 ELISA Kit (AB100731)

Representative standard curve using ab100731

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

13.72 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's MMP3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP3 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse MMP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "106 - 130 %", "average": "= 117.4" }, { "sample": "Serum", "range": "101 - 140 %", "average": "= 120.6" }, { "sample": "Plasma", "range": "101 - 137 %", "average": "= 111.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100731-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100731-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100731-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MMP-3 Microplate (12 x 8 wells)", "number":"AB100731-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100731-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse MMP-3 Standard (lyophilized)", "number":"AB100731-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse MMP-3", "number":"AB100731-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100731-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100731-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.
Biological function summary

MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.

Pathways

MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.

MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.
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Product protocols

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Target data

Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9. Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway. Acts also intracellularly. For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage (PubMed : 17116747). In addition, plays a role in immune response and possesses antiviral activity against various viruses (PubMed : 35940311). Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities (PubMed : 35940311).
See full target information Mmp3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 7:eabg4980 PubMed34669475

2021

Microglia become hypofunctional and release metalloproteases and tau seeds when phagocytosing live neurons with P301S tau aggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Jack H Brelstaff,Matthew Mason,Taxiarchis Katsinelos,William A McEwan,Bernardino Ghetti,Aviva M Tolkovsky,Maria Grazia Spillantini

Clinical and experimental immunology 186:177-189 PubMed27465496

2016

Porphyromonas gingivalis infection exacerbates the onset of rheumatoid arthritis in SKG mice.

Applications

Unspecified application

Species

Unspecified reactive species

M Yamakawa,K Ouhara,M Kajiya,S Munenaga,M Kittaka,S Yamasaki,K Takeda,K Takeshita,N Mizuno,T Fujita,E Sugiyama,H Kurihara
View all publications
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