AB203363

Mouse MMP3 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Mouse MMP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MMP3 with a sensitivity of 4.94 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Stromelysin-1, SL-1, EMS-2, Matrix metalloproteinase-3, Transin-1, MMP-3, Mmp3

3 Images

Sandwich ELISA - Mouse MMP3 ELISA Kit (AB203363)

sELISA

Supplier Data

Sandwich ELISA - Mouse MMP3 ELISA Kit (AB203363)

Example of MMP3 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse MMP3 ELISA Kit (AB203363)

Mouse MMP3 standard curve comparison data.

Standard curve comparison between mouse MMP3 SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 10-fold increase in sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse MMP3 ELISA Kit (AB203363)

Interpolated concentrations of MMP3 in mouse plasma (heparin and citrate) and serum.

The concentrations of MMP3 were measured in duplicate and interpolated from the MMP3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 4.94 pg/mL

Range

18.8 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse MMP3 ELISA Kit (ab203363) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP3 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MMP3 with 4.94 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Matrix metalloproteinase-3 (MMP3) is a member of the matrix metalloproteinase family, which are involved in breakdown of the extracellular matrix. MMP3 is secreted in a proenzyme form from a variety of cells, including macrophages, osteoblasts, keratinocytes, chondrocytes, fibroblasts and vascular smooth muscle cells. It degrades collagen types II, III, IV, IX and X, along with proteoglycans, elastin, laminin and fibronectin. In addition to normal physiological processes like development, reproduction and wound healing, MMP3 is implicated in humans in the progression of atherosclerosis and tumor initiation.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 109 %", "average": "= 103" }, { "sample": "Serum", "range": "101 - 126 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "97 - 110 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "97 - 115 %", "average": "= 105" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.

Biological function summary

MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.

Pathways

MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.

MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.

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Product protocols

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Target data

Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9. Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway. Acts also intracellularly. For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage (PubMed : 17116747). In addition, plays a role in immune response and possesses antiviral activity against various viruses (PubMed : 35940311). Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities (PubMed : 35940311).

See full target information Mmp3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:6655122 PubMed33859779

2021

Increase in Blood-Brain Barrier (BBB) Permeability Is Regulated by MMP3 via the ERK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Zhang,Mei Zheng,Cristian E Betancourt,Lifeng Liu,Albert Sitikov,Nikola Sladojevic,Qiong Zhao,John H Zhang,James K Liao,Rongxue Wu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse MMP3 ELISA Kit

Sandwich ELISA - Mouse MMP3 ELISA Kit (AB203363)

Sandwich ELISA - Mouse MMP3 ELISA Kit (AB203363)

Sandwich ELISA - Mouse MMP3 ELISA Kit (AB203363)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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