AB206982

Mouse MMP8 ELISA Kit

Recombinant
SimpleStep
What is this?

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(4 Publications)

Mouse MMP8 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MMP8 with a sensitivity of 53 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Neutrophil collagenase, Collagenase 2, Matrix metalloproteinase-8, MMP-8, Mmp8

2 Images

Sandwich ELISA - Mouse MMP8 ELISA Kit (AB206982)

sELISA

Supplier Data

Sandwich ELISA - Mouse MMP8 ELISA Kit (AB206982)

Interpolated concentrations of MMP8 in mouse serum, plasma (citrate), plasma (heparin) and plasma (EDTA).

The concentrations of MMP8 were measured in duplicate and interpolated from the MMP8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP8 concentration was determined to be 46.5 ng/mL in mouse serum, 45.2 ng/mL in mouse plasma (Citrate), 36.6 ng/mL in mouse plasma (heparin), and 20.2 ng/mL in mouse plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse MMP8 ELISA Kit (AB206982)

Example of MMP8 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 53 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse MMP8 ELISA Kit (ab206982) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP8 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MMP8 with 53 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MMP8 is a member of the matrix metalloproteinase family of zinc proteases that degrade extracellular matrix. MMP8 is expressed in neutrophils and is also known as neutrophil collagenase. MMP8 is expressed as a propeptide. This antibodies in this assay were raised to the mature portion of MMP8.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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Recovery

[ { "sample": "Serum", "range": "108 - 119 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "97 - 112 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "95 - 103 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "105 - 113 %", "average": "= 108" }, { "sample": "Cell culture media", "range": "98 - 105 %", "average": "= 100" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metalloproteinase-8 (MMP-8) also known as neutrophil collagenase is an enzyme with a molecular weight of approximately 57 kDa. It belongs to the matrix metalloproteinase family which is involved in the breakdown of extracellular matrix components. MMP-8 mainly expresses in neutrophils but can also be found in other tissues such as the skin lungs and liver. The expression of MMP-8 increases during inflammation and infection where it plays a critical role in tissue remodeling and repair processes.

Biological function summary

MMP-8 participates in the degradation of collagen types I II and III contributing to extracellular matrix remodeling. It acts independently rather than being part of a larger enzyme complex. Its activity is tightly regulated by tissue inhibitors of metalloproteinases (TIMPs) to prevent excessive tissue damage. By modulating the extracellular matrix MMP-8 influences processes like wound healing cell migration and angiogenesis indicating its essential role in maintaining tissue homeostasis.

Pathways

MMP-8 plays a role in the inflammatory response and wound healing pathways. It interacts with and processes other proteins like pro-inflammatory cytokines and growth factors which are important for regulating these pathways. MMP-8 collaborates with matrix metalloproteinase-9 (MMP-9) in the inflammatory pathway to facilitate leukocyte migration and rapid tissue remodeling during acute inflammatory responses. Its regulation involves complex interactions among proteolytic enzymes emphasizing its function in balancing matrix degradation and formation.

MMP-8 is associated with periodontal disease and rheumatoid arthritis. In periodontal disease elevated MMP-8 levels can lead to breakdown of connective tissue and bone contributing to tooth loss. In rheumatoid arthritis MMP-8 alongside MMP-1 degrades joint cartilage collagen exacerbating the inflammation and joint damage. These associations underline MMP-8's potential as a biomarker for these diseases and highlight the need for MMP-8 testing tools such as MMP8 ELISA kits to monitor disease progression and treatment efficacy.

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Product protocols

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Target data

Can degrade fibrillar type I, II, and III collagens. May play a role in the degradation of collagen fibers during uterine involution.

See full target information Mmp8

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36291087

2022

Targeted Inhibition of Matrix Metalloproteinase-8 Prevents Aortic Dissection in a Murine Model.

Applications

Unspecified application

Species

Unspecified reactive species

Chengxin Zhang,Kaiyuan Niu,Meixia Ren,Xinmiao Zhou,Zhisheng Yang,Mei Yang,Xinxin Wang,Jun Luo,Yue Shao,Cheng Zhang,Dan Chen,Shan Gao,Shenglin Ge,Qingchen Wu,Qingzhong Xiao

Nature communications 13:318 PubMed35031603

2022

Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Spix,Elisabeth S Butz,Cheng-Chang Chen,Anna Scotto Rosato,Rachel Tang,Aicha Jeridi,Veronika Kudrina,Eva Plesch,Philipp Wartenberg,Elisabeth Arlt,Daria Briukhovetska,Meshal Ansari,Gizem Günes Günsel,Thomas M Conlon,Amanda Wyatt,Sandra Wetzel,Daniel Teupser,Lesca M Holdt,Fabien Ectors,Ingrid Boekhoff,Ulrich Boehm,Jaime García-Añoveros,Paul Saftig,Martin Giera,Sebastian Kobold,Herbert B Schiller,Susanna Zierler,Thomas Gudermann,Christian Wahl-Schott,Franz Bracher,Ali Önder Yildirim,Martin Biel,Christian Grimm

Journal of pain research 12:1133-1138 PubMed31118746

2019

Spinal matrix metalloproteinase 8 regulates pain after peripheral trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Maral Tajerian,J David Clark

Immunology 155:72-84 PubMed29532462

2018

Murine neutrophils treated with alphaB-crystallin reduce IL-12p40 production by dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Trisha M Finlay,Alexandra L Palmer,Shalina S Ousman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse MMP8 ELISA Kit

Sandwich ELISA - Mouse MMP8 ELISA Kit (AB206982)

Sandwich ELISA - Mouse MMP8 ELISA Kit (AB206982)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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