JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB253227

Mouse MMP9 ELISA Kit

Be the first to review this product! Submit a review

|

(6 Publications)

Mouse MMP9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MMP9 with a sensitivity of 38.91 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Clg4b, Mmp9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB

7 Images
ELISA - Mouse MMP9 ELISA Kit (AB253227)
  • ELISA

Supplier Data

ELISA - Mouse MMP9 ELISA Kit (AB253227)
Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)
  • sELISA

Unknown

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)

Interpolated concentrations of native MMP9 in mouse J774A.1 cell culture supernatant samples.

The concentrations of MMP9 were measured in duplicates, interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows : J774A.1 cell culture supernatant 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 1326.73 ng/mL in J774A.1 cell culture supernatant.

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)
  • sELISA

Unknown

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)

Example of mouse MMP9 standard curve in Sample Diluent NS.

The MMP9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)
  • sELISA

Unknown

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)

Interpolated concentrations of native MMP9 in mouse LPS unstimulated and stimulated P388D1 cell culture supernatant samples.

The concentrations of MMP9 were measured in duplicates, interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated P388D1 supernatant 100% and LPS stimulated P388 D1 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 1.03 ng/mL in unstimulated P388D1 supernatant and 74.58 ng/mL in LPS stimulated P388D1.

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)
  • sELISA

Unknown

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)

Interpolated concentrations of native MMP9 in mouse serum and plasma - heparin samples.

The concentrations of MMP9 were measured in duplicates, interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 158.72 ng/mL in serum and 37.15 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)
  • sELISA

Unknown

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)

Interpolated concentrations of native MMP9 in mouse lung extract samples based on a 50 μg/mL extract load and spleen extract samples based on a 25 μg/mL extract load.

The concentrations of MMP9 were measured in duplicate and interpolated from the MMP9 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP9 concentration was determined to be 5.51 ng/mL in lung extract and 5.37 ng/mL in spleen extract.

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)
  • sELISA

Unknown

Sandwich ELISA - Mouse MMP9 ELISA Kit (AB253227)

Example of mouse MMP9 standard curve in 1X Cell Extraction Buffer PTR.

The MMP9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 38.91 pg/mL

Range

140.63 - 9000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Mouse MMP9 ELISA Kit ab253227 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse MMP9 in cell culture supernatant, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 38.91 pg/mL.

How the assay works

Mouse MMP9 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse MMP9 ELISA Kit ab253227 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MMP9 antibody [EPR22140-157] - BSA and Azide free (Capture) ab259708
- Anti-MMP9 antibody [EPR22140-151] - BSA and Azide free (Detector) ab259708

MMP9 (gelatinase B) is a member of the matrix metalloproteinases family of zinc and calcium dependent endopeptidases. MMP9 is a 95kDa protein produced by keratinocytes, monocytes, macrophages and PMN leukocytes. It is able to degrade all the components of the extracellular matrix including gelatin, collagen types IV and V, elastin and proteoglycan core protein. MMP9 is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP9 is present in most inflammatory responses. Mouse MMP9 shares 72.9% sequence homology with primate. 71.8% with human, 81.6 % with rat, and 73.0% with bovine MMP9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "92 - 117 %", "average": "= 106" }, { "sample": "Serum", "range": "90 - 100 %", "average": "= 94" }, { "sample": "Tissue Extracts", "range": "85 - 101 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "102 - 117 %", "average": "= 109" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab253227-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB253227-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB253227-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB253227-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB253227-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB253227-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB253227-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB253227-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MMP9 Capture Antibody", "number":"AB253227-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse MMP9 Lyophilized Recombinant Protein", "number":"AB253227-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB253227-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB253227-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MMP9 Detector Antibody", "number":"AB253227-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).
Biological function summary

Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.

Pathways

MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.

Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.
style
left-column

Product protocols

style
left-column

Target data

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (By similarity). Could play a role in bone osteoclastic resorption (PubMed : 8132709). Cleaves KiSS1 at a Gly-|-Leu bond (By similarity). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed : 23142597, PubMed : 32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide (By similarity).
See full target information Mmp9
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39273042

2024

Maternal Dietary Deficiency in Choline Reduced Levels of MMP-2 Levels in Blood and Brain Tissue of Male Offspring Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mitra Esfandiarei,Shawn G U Strash,Amanda Covaleski,Sharadyn Ille,Weidang Li,Nafisa M Jadavji

Nature communications 14:5529 PubMed37684243

2023

Fasting mimicking diet in mice delays cancer growth and reduces immunotherapy-associated cardiovascular and systemic side effects.

Applications

Unspecified application

Species

Unspecified reactive species

S Cortellino,V Quagliariello,G Delfanti,O Blaževitš,C Chiodoni,N Maurea,A Di Mauro,F Tatangelo,F Pisati,A Shmahala,S Lazzeri,V Spagnolo,E Visco,C Tripodo,G Casorati,P Dellabona,V D Longo

Folia neuropathologica 59:359-371 PubMed35114776

2022

USP38 might be a new therapeutic target for glioma via regulation of cancer cell metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Hu,Ke-Ni Wang,Hai-Rui LingHu

Frontiers in immunology 12:745854 PubMed34721415

2021

Endogenous α7 nAChR Agonist SLURP1 Facilitates K1 Crossing the Blood-Brain Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong He,Lei Wang,Liqun Liu,Jie Gao,Beiguo Long,Feng Chi,Tongtong Hu,Yu Wan,Zelong Gong,Li Li,Peilin Zhen,Tiesong Zhang,Hong Cao,Sheng-He Huang

Frontiers in immunology 12:623256 PubMed34381441

2021

A20 Establishes Negative Feedback With TRAF6/NF-κB and Attenuates Early Brain Injury After Experimental Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Ji Deng,QuZhen Deji,WangDui Zhaba,Jia-Qiang Liu,Sheng-Qing Gao,Yan-Ling Han,Meng-Liang Zhou,Chun-Xi Wang

Cardiovascular diabetology 20:150 PubMed34301253

2021

The SGLT-2 inhibitor empagliflozin improves myocardial strain, reduces cardiac fibrosis and pro-inflammatory cytokines in non-diabetic mice treated with doxorubicin.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Quagliariello,Michelino De Laurentiis,Domenica Rea,Antonio Barbieri,Maria Gaia Monti,Andreina Carbone,Andrea Paccone,Lucia Altucci,Mariarosaria Conte,Maria Laura Canale,Gerardo Botti,Nicola Maurea
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB254516

Mouse MMP-2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse MMP-2 ELISA Kit (AB254516)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB206982

Mouse MMP8 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse MMP8 ELISA Kit (AB206982)

  • 0
  • 0
Assay Kits

AB203363

Mouse MMP3 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse MMP3 ELISA Kit (AB203363)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com