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AB155458

Mouse MPO ELISA Kit (Myeloperoxidase)

5

(2 Reviews)

|

(32 Publications)

Mouse MPO ELISA Kit (Myeloperoxidase) is a sandwich ELISA designed to quantify Mouse MPO (Myeloperoxidase) with a sensitivity of 0.6 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.61 - 150 ng/mL
- Cited in over 20 publications

View Alternative Names

Myeloperoxidase, MPO, Mpo

2 Images
Sandwich ELISA - Mouse MPO ELISA Kit (Myeloperoxidase) (AB155458)
  • sELISA

Unknown

Sandwich ELISA - Mouse MPO ELISA Kit (Myeloperoxidase) (AB155458)

Representative standard curve using ab155458 - Assay Diluent B.

Sandwich ELISA - Mouse MPO ELISA Kit (Myeloperoxidase) (AB155458)
  • sELISA

Unknown

Sandwich ELISA - Mouse MPO ELISA Kit (Myeloperoxidase) (AB155458)

Representative standard curve using ab155458 - Assay Diluent C.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

<= 0.61 ng/mL

Range

0.61 - 150 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse MPO ELISA Kit (Myeloperoxidase) ab155458 is a sandwich ELISA to measure Mouse MPO (Myeloperoxidase) in plasma, cell culture supernatant, cell lysate with a sensitivity of 0.6 ng/ml.

How the assay works

This assay employs an antibody specific for mouse MPO coated on a 96-well plate. Standards and samples are pipetted into the wells and MPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse MPO ELISA Kit (Myeloperoxidase) ab155458 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "96 - 112 %", "average": "= 105.5" }, { "sample": "Plasma", "range": "68 - 105 %", "average": "= 86.89" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155458-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155458-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155458-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155458-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB155458-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155458-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse MPO", "number":"AB155458-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse MPO Standard (lyophilized)", "number":"AB155458-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MPO Microplate (12 x 8 wells)", "number":"AB155458-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155458-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
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Product protocols

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 11593004). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low density lipoprotein particles and limits vascular damage (By similarity).
See full target information Mpo
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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:339 PubMed40340852

2025

Dual-responsive nanoparticles targeting ACE-II senescence for therapeutic mitigation of acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Gao,Fushuang Zheng,Zhiling Fu,Wei Wang

Innate immunity 31:17534259251335770 PubMed40241483

2025

Lipopolysaccharide preconditioning augments the antibacterial activity of renal macrophages and ameliorates acute kidney injury caused by bacteremia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Keiko Tanoue,Manabu Kinoshita,Bradley M Kearney,Seigo Ito,Hiroyasu Goto,Aoi Yamashiro,Tsugumi Fukunaga,Hiroki Sato,Kazuma Mori,Koji Kuwata,Hidehito Matsubara,Azusa Kato,Masahiro Nakashima,Hiroyuki Nakashima,Toshihiko Imakiire,Naoki Oshima

Pulmonary circulation 14:e12454 PubMed39386377

2024

Didang decoction attenuates cancer-associated thrombosis by inhibiting PAD4-dependent NET formation in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Zeng,Jiuxi Li,Liyuan Pei,Yaping Yang,Ya Chen,Xuejing Wang,Ting Zhang,Ting Zhou

Cell reports 43:114607 PubMed39126652

2024

Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection.

Applications

Unspecified application

Species

Unspecified reactive species

Mack B Reynolds,Benjamin Klein,Michael J McFadden,Norah K Judge,Hannah E Navarrete,Britton C Michmerhuizen,Dominik Awad,Tracey L Schultz,Paul W Harms,Li Zhang,Teresa R O'Meara,Jonathan Z Sexton,Costas A Lyssiotis,J Michelle Kahlenberg,Mary X O'Riordan

mBio 15:e0140924 PubMed38953359

2024

promotes inflammasome formation and NETosis during pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Steven G Sayson,Alan Ashbaugh,Aleksey Porollo,George Smulian,Melanie T Cushion

International journal of molecular medicine 52: PubMed37772380

2023

Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Li,Hua Huang,Ping Zhao,Jie Jiang,Xufeng Ding,Donxgue Lu,Lijiang Ji

Nucleic acids research 51:2195-2214 PubMed36794705

2023

p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxing Zhu,Beijia Huang,Fengting Zhao,Jie Lian,Lixiang He,Yangxia Zhang,Longkai Ji,Jinghang Zhang,Xin Yan,Taoling Zeng,Chunya Ma,Yinming Liang,Chen Zhang,Juntang Lin

Molecular therapy : the journal of the American Society of Gene Therapy 31:1365-1382 PubMed36733250

2023

Extracellular vesicle-transmitted miR-671-5p alleviates lung inflammation and injury by regulating the AAK1/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Lian,Xinxing Zhu,Jiang Du,Beijia Huang,Fengting Zhao,Chunya Ma,Rui Guo,Yangxia Zhang,Longkai Ji,Badrul Hisham Yahaya,Juntang Lin

Evidence-based complementary and alternative medicine : eCAM 2022:3676444 PubMed36588594

2022

Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Dong,Ying Zhang,Nian Liu,Jun-Yuan Yang,Hui-Min Wang,Qing Geng

Oxidative medicine and cellular longevity 2022:8659587 PubMed35602094

2022

MicroRNA-299a-5p Protects against Spinal Cord Injury through Activating AMPK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zong-Ze Zhang,Shu-Yue Xian,Chong Bao,Feng Chen
View all publications
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websiteProtocolBooklet
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Product promise

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