JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213879

Mouse MSP ELISA Kit (MST1)

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse MSP ELISA Kit (MST1) is a Sandwich ELISA for the measurement of Mouse MSP (MST1) in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Hgfl, Mst1, Hepatocyte growth factor-like protein, Macrophage stimulatory protein, MSP

1 Images
Sandwich ELISA - Mouse MSP ELISA Kit (MST1) (AB213879)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse MSP ELISA Kit (MST1) (AB213879)

Mouse MSP ELISA Kit (ab213879) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB252362

Human CD30-L ELISA Kit (TNFSF8)

Recombinant|SimpleStep

ELISA - Human CD30-L ELISA Kit (TNFSF8) (AB252362)

  • 0
  • 0
Assay Kits

AB252355

Mouse ICAM 1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse ICAM 1 ELISA Kit (AB252355)

  • 0
  • 0
Primary Antibodies

AB245190

Anti-MSP/MST1 antibody [EPR22184-180]

RabMAb|Recombinant

Western blot - Anti-MSP/MST1 antibody [EPR22184-180] (AB245190)

  • 0
  • 0
Assay Kits

AB200016

Human Cardiac Troponin I ELISA Kit

Recombinant|SimpleStep

ELISA - Human Cardiac Troponin I ELISA Kit (AB200016)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Mouse MSP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213879) is designed for the quantitative measurement of Mouse MSP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for MSP/MST1 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: Q19-E716) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MSP/MST1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MSP/MST1 amount of sample captured in plate.

Macrophage Stimulating 1 (MST1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase, the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1513 pg/mL", "standardDeviation": "62.03", "coefficientOfVariability": "= 4.1" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "5860 pg/mL", "standardDeviation": "322.3", "coefficientOfVariability": "= 5.5" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "276 pg/mL", "standardDeviation": "12.97", "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1456 pg/mL", "standardDeviation": "61.15", "coefficientOfVariability": "= 4.2" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "5996 pg/mL", "standardDeviation": "425.71", "coefficientOfVariability": "= 7.1" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "257 pg/mL", "standardDeviation": "11.56", "coefficientOfVariability": "= 4.5" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213879-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213879-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213879-CMP08", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Mouse MSP antibody", "number":"AB213879-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse MSP coated Microplate (12 x 8 wells)", "number":"AB213879-CMP04", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213879-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213879-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213879-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Mouse MSP standard", "number":"AB213879-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213879-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213879-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213879-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MSP/MST1 also known as macrophage stimulating protein is a serine protease family protein with a molecular mass of approximately 80 kDa. It expresses in various tissues with significant presence in liver tissue. MST1 is a precursor protein that undergoes proteolytic cleavage to become its active form. MSP protein plays an important role in signaling processes acting as a ligand for the RON receptor tyrosine kinase triggering downstream effects in various cellular systems.
Biological function summary

MSP protein contributes to numerous cellular events including migration differentiation and survival. It forms a complex with the RON receptor facilitating cell signaling cascades that influence these processes. MSP is key in the regulation of immune responses and tissue remodeling which makes it significant in inflammation and wound healing contexts. It influences macrophage activity promoting their ability to clear pathogens and repair tissues.

Pathways

MSP protein is integral to the HGF/MET signaling pathway and the related MSP/RON pathway. These pathways are involved in cellular growth motility and morphogenesis with a notable impact on epithelial cellular dynamics. MST1 through its interaction with RON influences c-Met and related pathways that intertwine with cell cycle regulation. The crosstalk with hepatocyte growth factor (HGF) can further elucidate the sophisticated regulation of cellular functions where MSP plays a role.

MSP protein has been implicated in cancer progression and inflammatory diseases. It associates closely with the progression of certain carcinomas through its interaction with the RON receptor influencing tumor proliferation and metastasis. Additionally the dysregulation of MSP is associated with chronic inflammatory conditions linking it to key pathway proteins such as HGF. The therapeutic targeting of MSP might offer insights into anticancer and anti-inflammatory treatment strategies.
style
left-column

Product protocols

style
left-column

Target data

See full target information Mst1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:14265 PubMed29079854

2017

Identification of MST1 as a potential early detection biomarker for colorectal cancer through a proteomic approach.

Applications

Unspecified application

Species

Unspecified reactive species

Jiekai Yu,Xiaohui Zhai,Xiaofen Li,Chenhan Zhong,Cheng Guo,Fuquan Yang,Ying Yuan,Shu Zheng
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com