Mouse MSP ELISA Kit (MST1) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse MSP (MST1) in Mouse in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
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- Scientific reports 7:142652017Identification of MST1 as a potential early detection biomarker for colorectal cancer through a proteomic approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiekai Yu,Xiaohui Zhai,Xiaofen Li,Chenhan Zhong,Cheng Guo,Fuquan Yang,Ying Yuan,Shu ZhengPubMed 29079854
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156 - 10000 pg/mL
- Assay time
- 3h 30m
- Sensitivity
- < 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
Hgfl, Mst1, Hepatocyte growth factor-like protein, Macrophage stimulatory protein, MSP
What's included?
Recommended products
Mouse MSP ELISA Kit (MST1) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse MSP (MST1) in Mouse in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156 - 10000 pg/mL
- Assay time
- 3h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- < 10 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 2 | n 16 | mean 1513 pg/mL | SD 62.03 | C.V. = 4.1 |
Sample Sample 3 | n 16 | mean 5860 pg/mL | SD 322.3 | C.V. = 5.5 |
Sample Sample 1 | n 16 | mean 257 pg/mL | SD 11.56 | C.V. = 4.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 24 | mean 276 pg/mL | SD 12.97 | C.V. = 4.7 |
Sample Sample 2 | n 24 | mean 1456 pg/mL | SD 61.15 | C.V. = 4.2 |
Sample Sample 3 | n 24 | mean 5996 pg/mL | SD 425.71 | C.V. = 7.1 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
The Mouse MSP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213879) is designed for the quantitative measurement of Mouse MSP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for MSP/MST1 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: Q19-E716) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MSP/MST1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MSP/MST1 amount of sample captured in plate.
Macrophage Stimulating 1 (MST1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase, the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MSP/MST1 also known as macrophage stimulating protein is a serine protease family protein with a molecular mass of approximately 80 kDa. It expresses in various tissues with significant presence in liver tissue. MST1 is a precursor protein that undergoes proteolytic cleavage to become its active form. MSP protein plays an important role in signaling processes acting as a ligand for the RON receptor tyrosine kinase triggering downstream effects in various cellular systems.
- Biological function summary
MSP protein contributes to numerous cellular events including migration differentiation and survival. It forms a complex with the RON receptor facilitating cell signaling cascades that influence these processes. MSP is key in the regulation of immune responses and tissue remodeling which makes it significant in inflammation and wound healing contexts. It influences macrophage activity promoting their ability to clear pathogens and repair tissues.
- Pathways
MSP protein is integral to the HGF/MET signaling pathway and the related MSP/RON pathway. These pathways are involved in cellular growth motility and morphogenesis with a notable impact on epithelial cellular dynamics. MST1 through its interaction with RON influences c-Met and related pathways that intertwine with cell cycle regulation. The crosstalk with hepatocyte growth factor (HGF) can further elucidate the sophisticated regulation of cellular functions where MSP plays a role.
- Associated diseases and disorders
MSP protein has been implicated in cancer progression and inflammatory diseases. It associates closely with the progression of certain carcinomas through its interaction with the RON receptor influencing tumor proliferation and metastasis. Additionally the dysregulation of MSP is associated with chronic inflammatory conditions linking it to key pathway proteins such as HGF. The therapeutic targeting of MSP might offer insights into anticancer and anti-inflammatory treatment strategies.
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Sandwich ELISA - Mouse MSP ELISA Kit (MST1) (ab213879)
Mouse MSP ELISA Kit (ab213879) Standard Curve.
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