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AB284028

Mouse MUP1 ELISA Kit

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Mouse MUP1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse MUP1 in Mouse in Biofluids samples.

View Alternative Names

Major urinary protein 1, MUP 1, Mup1

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Sandwich ELISA - Mouse MUP1 ELISA Kit (AB284028)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse MUP1 ELISA Kit (AB284028)

Example of Mouse MUP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 79 pg/mL

Range

0.125 - 8 ng/mL

Assay time

4h

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Mouse Mup1 ELISA Kit (ab284028) is designed for detection of Mup1 in mouse plasma, serum, urine, and cell culture samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures mouse Mup1 in approximately 4 hours. A polyclonal antibody specific for mouse Mup1 has been pre-coated onto a 96-well microplate with removable strips. Mup1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for mouse Mup1, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Mup1 protein also known as Major Urinary Protein 1 has a molecular mass of approximately 19 kDa. It is expressed predominantly in the liver and secreted into the urine where it functions as a pheromone carrier. Mup1 is part of a large family of proteins called lipocalins known for their role in binding small hydrophobic molecules. In rodents Mup1 binds to volatile pheromones which are important for communication. Its expression pattern and functional role make Mup1 a significant protein for research concerning biochemical signaling mechanisms.
Biological function summary

Mup1 plays a substantial role in the modulation of pheromone signaling interacting with receptors in target organisms. It is not part of larger protein complexes but functions independently to facilitate chemical communication. Mup1's role extends beyond simple pheromone transport as it impacts mating behaviors and territorial marking in rodents. By carrying specific ligands Mup1 influences reproductive signaling and social interaction defining its importance in ecological studies.

Pathways

Mup1 influences pheromonal communication and social behavior pathways in mammals. It significantly interacts with the olfactory signaling pathway where it binds to receptors such as V1R and V2R on sensory neurons. The activity of Mup1 in these pathways highlights its connection to the neural circuits responsible for detecting social cues linking it to other proteins involved in olfactory signal transduction.

Mup1 has shown links to metabolic regulation disorders particularly obesity. Altered Mup1 expression affects glucose metabolism and energy homeostasis connecting it to proteins like leptin and adiponectin known for their roles in metabolic processes. Furthermore research indicates potential links between Mup1 and neurological disorders related to social behavior though the exact mechanisms remain under investigation. These associations highlight the significance of Mup1 in the context of both metabolic and neurobehavioral studies.
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Product protocols

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Target data

Binds pheromones that are released from drying urine of males. These pheromones affect the sexual behavior of females.
See full target information Major urinary protein 1
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB95198

Anti-Mup1 antibody

Western blot - Anti-Mup1 antibody (AB95198)

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Proteins & Peptides

AB95193

Recombinant Mouse Mup1 protein

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