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AB285307

Mouse Myeloperoxidase ELISA Kit

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(2 Publications)

Mouse Myeloperoxidase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Myeloperoxidase in Mouse in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Myeloperoxidase, MPO, Mpo

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Sandwich ELISA - Mouse Myeloperoxidase ELISA Kit (AB285307)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Myeloperoxidase ELISA Kit (AB285307)

Mouse Myeloperoxidase ELISA Kit.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 18.75 pg/mL

Range

31.2 - 2000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Myeloperoxidase ELISA Kit (ab285307, E4580) is a sandwich ELISA assay for the quantitative measurement of MPO in Mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of MPO captured from the samples.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
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Product protocols

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 11593004). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low density lipoprotein particles and limits vascular damage (By similarity).
See full target information Mpo
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 22:101 PubMed40241182

2025

Cytomegalovirus infection initiates inflammatory bowel disease by activating a positive MyD88/NF-κB feedback loop in allogeneic skin transplantation mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Xian Chen,Yu Chen,Rui Fu,Jie-Yi Wang,Sai-Yue Liu,Tang-Biao Shen

JCI insight 10: PubMed39836475

2025

Neutrophils initiate proinflammatory immune responses in early endometriosis lesion development.

Applications

Unspecified application

Species

Unspecified reactive species

Taylor R Wilson,Kurt R Peterson,Stephanie A Morris,Damaris Kuhnell,Susan Kasper,Katherine A Burns
View all publications
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