AB234575

Mouse NBL1 ELISA Kit

Recombinant
SimpleStep
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Mouse NBL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse NBL1 with a sensitivity of 21.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Dan, Dana, Nbl1, Neuroblastoma suppressor of tumorigenicity 1, N03, Zinc finger protein DAN

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Sandwich ELISA - Mouse NBL1 ELISA Kit (AB234575)

sELISA

Supplier Data

Sandwich ELISA - Mouse NBL1 ELISA Kit (AB234575)

Interpolated concentrations of native NBL1 in mouse serum samples.

The concentrations of NBL1 were measured in duplicates, interpolated from the NBL1 standard curves and corrected for sample dilution. Samples were loaded at 4% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NBL1 concentration was determined to be 63.4 ng/mL in mouse serum.

sELISA

Supplier Data

Sandwich ELISA - Mouse NBL1 ELISA Kit (AB234575)

Example of mouse NBL1 standard curve in Sample Diluent NS + Enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse NBL1 ELISA Kit (AB234575)

Representative image of Mouse NBL1 ELISA Kit (ab234575)

Components shown from left to right :

- Sample diluent NS

- 10X Mouse NBL1 Capture Antibody

- 10X Mouse NBL1 Detector Antibody

- Mouse NBL1 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent CPR2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 21.6 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse NBL1 ELISA Kit (ab234575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NBL1 protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NBL1 with 21.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]

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Recovery

[ { "sample": "Serum", "range": null, "average": "= 101" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234575-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB234575-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse NBL1 Capture Antibody", "number":"AB234575-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234575-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234575-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse NBL1 Lyophilized Recombinant Protein", "number":"AB234575-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234575-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234575-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234575-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB234575-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse NBL1 Detector Antibody", "number":"AB234575-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234575-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NBL1 also known as DAN domain family member 1 is a protein with a molecular weight of approximately 20 kDa. It is found in various tissues but shows higher expression levels in the nervous tissue and skeletal muscle. Mechanically NBL1 acts as a modulator in cell signaling pathways by binding to specific growth factors such as BMPs (Bone Morphogenetic Proteins) inhibiting their functions and affecting cell differentiation and growth.

Biological function summary

NBL1 plays an inhibitory role in bone morphogenesis by interacting with BMPs which are part of the TGF-beta superfamily. NBL1 can form complexes with these growth factors thereby regulating processes related to bone and cartilage development. This protein is essential for maintaining cellular processes involved in tissue homeostasis and suppressing unchecked cellular proliferation.

Pathways

NBL1 involves itself in the BMP signaling pathway where it directly impacts the regulation of bone and cartilage development. It is associated with other proteins like Noggin and Chordin both of which also modulate BMP activity highlighting its importance in balancing cell growth and differentiation signals. The involvement of NBL1 in the TGF-beta/BMP pathway is critical for embryonic development and the regulation of body patterning.

NBL1 has been linked to conditions such as osteoarthritis and certain cancer types. Its inhibitory effect on BMPs associates it with osteoarthritis where altered BMP signaling contributes to cartilage degradation. In cancer where BMP activity can be dysregulated NBL1's role in inhibiting BMP pathways places it in connection to tumor suppressors like SMAD proteins emphasizing its potential relevance in tumorigenesis and metastasis.

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Product protocols

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Target data

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

See full target information Nbl1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse NBL1 ELISA Kit

Sandwich ELISA - Mouse NBL1 ELISA Kit (AB234575)

Sandwich ELISA - Mouse NBL1 ELISA Kit (AB234575)

Sandwich ELISA - Mouse NBL1 ELISA Kit (AB234575)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Product promise