Mouse NBL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse NBL1 with a sensitivity of 21.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 21.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
Alternative names
Dan, Dana, Nbl1, Neuroblastoma suppressor of tumorigenicity 1, N03, Zinc finger protein DAN
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Mouse NBL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse NBL1 with a sensitivity of 21.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 21.6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Protein | n 8 | mean - | SD - | C.V. 3.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 101 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse NBL1 ELISA Kit (ab234575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NBL1 protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NBL1 with 21.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NBL1 also known as DAN domain family member 1 is a protein with a molecular weight of approximately 20 kDa. It is found in various tissues but shows higher expression levels in the nervous tissue and skeletal muscle. Mechanically NBL1 acts as a modulator in cell signaling pathways by binding to specific growth factors such as BMPs (Bone Morphogenetic Proteins) inhibiting their functions and affecting cell differentiation and growth.
- Biological function summary
NBL1 plays an inhibitory role in bone morphogenesis by interacting with BMPs which are part of the TGF-beta superfamily. NBL1 can form complexes with these growth factors thereby regulating processes related to bone and cartilage development. This protein is essential for maintaining cellular processes involved in tissue homeostasis and suppressing unchecked cellular proliferation.
- Pathways
NBL1 involves itself in the BMP signaling pathway where it directly impacts the regulation of bone and cartilage development. It is associated with other proteins like Noggin and Chordin both of which also modulate BMP activity highlighting its importance in balancing cell growth and differentiation signals. The involvement of NBL1 in the TGF-beta/BMP pathway is critical for embryonic development and the regulation of body patterning.
- Associated diseases and disorders
NBL1 has been linked to conditions such as osteoarthritis and certain cancer types. Its inhibitory effect on BMPs associates it with osteoarthritis where altered BMP signaling contributes to cartilage degradation. In cancer where BMP activity can be dysregulated NBL1's role in inhibiting BMP pathways places it in connection to tumor suppressors like SMAD proteins emphasizing its potential relevance in tumorigenesis and metastasis.
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2 product images
Sandwich ELISA - Mouse NBL1 ELISA Kit (ab234575)
Example of mouse NBL1 standard curve in Sample Diluent NS + Enhancer.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse NBL1 ELISA Kit (ab234575)
Interpolated concentrations of native NBL1 in mouse serum samples.
The concentrations of NBL1 were measured in duplicates, interpolated from the NBL1 standard curves and corrected for sample dilution. Samples were loaded at 4% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NBL1 concentration was determined to be 63.4 ng/mL in mouse serum.
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