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AB267581

Mouse Neprilysin ELISA Kit

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Mouse Neprilysin ELISA Kit is a Sandwich ELISA for the measurement of Mouse Neprilysin in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD10, Neprilysin, Atriopeptidase, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, NEP, Neutral endopeptidase, SFE, Mme

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Sandwich ELISA - Mouse Neprilysin ELISA Kit (AB267581)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Neprilysin ELISA Kit (AB267581)

Mouse Neprilysin ELISA kit (ab267581) Standard curve.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 61 pg/mL

Range

61.44 - 15000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Neprilysin ELISA Kit is designed for the quantitative determination of Neprilysin in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for mouse Neprilysin coated on a 96-well plate. Standards and samples are pipetted into the wells and Neprilysin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Neprilysin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neprilysin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP1alpha, MIP-1gamma, MIP-2, MIP-3beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF1alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "70 - 105 %", "average": "= 77" }, { "sample": "Plasma", "range": "77 - 99 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "93 - 119 %", "average": "= 108" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267581-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267581-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267581-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267581-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse Neprilysin Detection Antibody", "number":"AB267581-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB267581-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse Neprilysin coated Microplate (12 x 8 wells)", "number":"AB267581-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Neprilysin Standard (Lyophilized)", "number":"AB267581-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267581-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD10 also known as neprilysin or neutral endopeptidase is a zinc-dependent metalloprotease enzyme with a mass of approximately 100 kDa. This enzyme is expressed across a variety of tissues including kidney lung and neutrophils. Its expression is also notable in the lymphoid tissues. CD10 functions by cleaving peptides at the N-terminal of hydrophobic residues. Common names used in literature for CD10 include CALLA (common acute lymphoblastic leukemia antigen) and membrane metalloendopeptidase.
Biological function summary

CD10 regulates peptide signaling by inactivating various bioactive peptides such as enkephalins atrial natriuretic peptide and bradykinin. It does not function as part of a complex but rather as a standalone entity. The ability of CD10 to hydrolyze peptides influences signal transmission across cellular membranes impacting cellular communication and signal termination within different systems.

Pathways

Neprilysin plays an essential role in hydrolyzing signaling peptides in several critical pathways. It is especially significant in the natriuretic peptide system and the renin-angiotensin system maintaining blood pressure and fluid balance. Within these pathways CD10 modifies peptides working alongside proteins like atrial natriuretic peptide synthase and angiotensin-converting enzyme ensuring systemic homeostasis and cardiovascular health.

CD10 shows a significant connection to certain types of cancer and Alzheimer's disease. In oncology altered expression of CD10 serves as a CD10 marker in cancers such as lymphomas and some leukemia forms which helps in diagnosis and treatment decisions. Additionally CD10's involvement in neuropeptide degradation links it to Amyloid beta peptide accumulation in Alzheimer's where its dysregulation may promote disease progression. Related proteins are amyloid precursor protein in Alzheimer's and CD19 often co-expressed with CD10 in some lymphomas highlighting its role in both diagnostic and therapeutic contexts.
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Product protocols

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Target data

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (By similarity). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (By similarity). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (By similarity). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (By similarity). Involved in the degradation of the atrial natriuretic factor (ANF) (By similarity). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed : 20876573).
See full target information Mme
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websiteProtocolBooklet
en
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Product promise

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