JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213882

Mouse Neurotrophin-3 ELISA Kit

Be the first to review this product! Submit a review

|

(5 Publications)

Mouse Neurotrophin-3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Neurotrophin-3 in Mouse in Cell Culture Media, Biofluids samples.

View Alternative Names

HDNF, MGC129711, NGF-2, NT-3, NTF3_HUMAN, Nerve growth factor 2, Neurotrophic factor, Neurotrophin 3 precursor

1 Images
Sandwich ELISA - Mouse Neurotrophin-3 ELISA Kit (AB213882)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Neurotrophin-3 ELISA Kit (AB213882)

Mouse Neurotrophin-3 ELISA Kit (ab213882) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Mouse Neurotrophin-3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213882) is designed for the quantitative measurement of Mouse Neurotrophin-3 in cell culture supernatants and serum.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Neurotrophin-3 amount of sample captured in plate.

Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "113 pg/mL", "standardDeviation": "7.12", "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "395 pg/mL", "standardDeviation": "28.44", "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "753 pg/mL", "standardDeviation": "39.16", "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "88 pg/mL", "standardDeviation": "4.84", "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "329 pg/mL", "standardDeviation": "19.74", "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "682 pg/mL", "standardDeviation": "30.01", "coefficientOfVariability": "4.4" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213882-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213882-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Mouse Neurotrophin-3 antibody", "number":"AB213882-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse Neurotrophin-3 coated Microplate (12 x 8 wells)", "number":"AB213882-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213882-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213882-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213882-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213882-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Mouse Neurotrophin-3 standard", "number":"AB213882-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213882-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213882-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213882-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurotrophin 3 also known as NT-3 or neurotrophin-3 is a protein with a molecular mass around 28 kDa. It belongs to the neurotrophin family and acts mechanically by binding to specific tyrosine kinase receptors especially TrkC on target cells. This binding initiates intracellular signaling cascades essential for neuronal development and survival. NT-3 is expressed in the central and peripheral nervous systems and is involved in various cellular processes. Expression levels can vary depending upon developmental stages and types of nervous tissue.
Biological function summary

NT-3 has a role in the survival and differentiation of neurons especially sensory ganglia neurons. It supports the development and maintenance of the nervous system by influencing neuron growth and plasticity. While not typically forming a large protein complex NT-3 interacts with its receptor TrkC to transmit critical signals internally. Such interactions can modulate synaptic responses and connectivity ultimately influencing learning and memory formation. NT-3 also interacts with p75NTR another receptor though this interaction is less specific compared to its action on TrkC.

Pathways

NT-3 participates in the MAPK/ERK signaling and PI3K/AKT pathways. These pathways are fundamental in regulating cell differentiation growth and survival. They also mediate a variety of cellular responses to external stimuli. NT-3's activation of the TrkC receptor triggers these pathways affecting other proteins such as BDNF (brain-derived neurotrophic factor) and NGF (nerve growth factor) which are part of the neurotrophin family. The intricate web of interactions highlights NT-3's significance in pathway modulation involved in neurodevelopment and plasticity.

NT-3 has implications in neurodegenerative diseases like Parkinson’s disease and Huntington's disease. The protein’s role in supporting neuronal survival points to its potential impact when its signaling is disrupted or deficient. NT-3 exhibits connections with proteins such as TrkC and BDNF which are also related to these neurodegenerative disorders. Dysfunctional NT-3-TrkC signaling could contribute to disease progression by impairing neuronal survival or recovery. Understanding NT-3's role in these contexts can offer insights into therapeutic approaches for such conditions.
style
left-column

Product protocols

style
left-column

Target data

See full target information Neurotrophin-3
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 10:892969 PubMed35721868

2022

Improving Control of Gene Therapy-Based Neurotrophin Delivery for Inner Ear Applications.

Applications

Unspecified application

Species

Unspecified reactive species

Madeleine St Peter,Douglas E Brough,Anna Lawrence,Jennifer Nelson-Brantley,Peixin Huang,Jennifer Harre,Athanasia Warnecke,Hinrich Staecker

Frontiers in cellular neuroscience 15:817277 PubMed35237130

2022

Vav3-Deficient Astrocytes Enhance the Dendritic Development of Hippocampal Neurons in an Indirect Co-culture System.

Applications

Unspecified application

Species

Unspecified reactive species

David Wegrzyn,Josephine Zokol,Andreas Faissner

International journal of molecular sciences 22: PubMed34445251

2021

Enhanced Nerve Regeneration by Exosomes Secreted by Adipose-Derived Stem Cells with or without FK506 Stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Shyuan Rau,Pao-Jen Kuo,Shao-Chun Wu,Lien-Hung Huang,Tsu-Hsiang Lu,Yi-Chan Wu,Chia-Jung Wu,Chia-Wei Lin,Chia-Wen Tsai,Ching-Hua Hsieh

Folia neuropathologica 58:176-193 PubMed32729296

2020

Tanshinone IIA alleviates the damage of neurocytes by targeting GLUT1 in ischaemia reperfusion model (in vivo and in vitro experiments).

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Haibo Tong,Xiangyang Wang,Xinxing Wang,Yang Wang

Cells, tissues, organs 208:89-100 PubMed32460289

2020

Neuropathic Pain Causes a Decrease in the Dendritic Tree Complexity of Hippocampal CA3 Pyramidal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Anna A Tyrtyshnaia,Igor V Manzhulo,Sophia P Konovalova,Anna A Zagliadkina
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB208348

Mouse TNF alpha ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

  • 4
  • 4
Assay Kits

AB222503

Mouse IL-6 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

  • 5
  • 2
Primary Antibodies

AB245249

Anti-LINE-1 ORF1p antibody [EPR22227-6]

RabMAb|Recombinant

Western blot - Anti-LINE-1 ORF1p antibody [EPR22227-6] (AB245249)

  • 0
  • 0
Assay Kits

AB197742

Mouse IL-1 beta ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com