AB252356

Mouse Neutrophil Elastase ELISA Kit

Recombinant
SimpleStep
What is this?

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(12 Publications)

Mouse Neutrophil Elastase ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Neutrophil Elastase with a sensitivity of 27.39 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Ela2, Elane, Neutrophil elastase, Elastase-2, Leukocyte elastase

6 Images

ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

ELISA

Supplier Data

ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

sELISA

Unknown

Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Example of mouse Neutrophil Elastase standard curve in 1X Cell Extraction Buffer PTR.

The Neutrophil Elastase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Interpolated concentrations of native Neutrophil Elastase in mouse serum and plasma samples.

The concentrations of Neutrophil Elastase were measured in duplicates, interpolated from the Neutrophil Elastase standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 40%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neutrophil Elastase concentration was determined to be 32.45 ng/mL in serum, 6.06 ng/mL in plasma (citrate) and 15.17 ng/mL in plasma (EDTA) and 17.69 ng/mL plasma (heparin).

sELISA

Unknown

Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Interpolated concentrations of native Neutrophil Elastase in mouse cell and tissue culture supernatant samples.

The concentrations of Neutrophil Elastase were measured in duplicates, interpolated from the Neutrophil Elastase standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC cell culture supernatant 100% and lung tissue culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neutrophil Elastase concentration was determined to be 500.61 pg/mL in PBMC supernatant and 20.83 ng/mL in lung supernatant.

sELISA

Unknown

Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Interpolated concentrations of native Neutrophil Elastase in mouse stomach extract (1000 ug/mL) and bone marrow extract (0.5 ug/mL) based on sample load.

The concentrations of Neutrophil Elastase were measured in duplicate and interpolated from the Neutrophil Elastase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neutrophil Elastase concentration was determined to be 2.02 ng/mL in stomach extract and 3.78 ng/mL in bone marrow extract.

sELISA

Unknown

Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Example of mouse Neutrophil Elastase standard curve in Sample Diluent NS.

The Neutrophil Elastase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Tissue Culture Media, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 27.39 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Neutrophil Elastase ELISA Kit ab252356 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Neutrophil Elastase in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue. This SimpleStep sensitivity is 27.39 pg/mL.

How the assay works

Mouse Neutrophil Elastase SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Neutrophil Elastase ELISA Kit ab252356 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Neutrophil Elastase antibody [EPR22231-229] - BSA and Azide free (Capture) ab259648
- Anti-Neutrophil Elastase antibody [EPR22231-232] - BSA and Azide free (Detector) ab259648

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 112 %", "average": "= 107" }, { "sample": "Serum", "range": "99 - 116 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "88 - 105 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "89 - 105 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "84 - 94 %", "average": "= 87" }, { "sample": "Tissue Extracts", "range": "84 - 92 %", "average": "= 87" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab252356-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB252356-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB252356-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB252356-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB252356-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB252356-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB252356-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Neutrophil Elastase Lyophilized Recombinant Protein", "number":"AB252356-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB252356-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Neutrophil Elastase Capture Antibody", "number":"AB252356-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Neutrophil Elastase Detector Antibody", "number":"AB252356-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB252356-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB252356-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.

Biological function summary

The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.

Pathways

This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.

Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.

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Product protocols

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Target data

Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity). Promotes blood coagulation (PubMed : 20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (By similarity). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (By similarity). Capable of killing E.coli; probably digests outer membrane protein A (ompA) in E.coli (PubMed : 10947984).

See full target information Elane

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70461 PubMed40457526

2025

Intra-Arterial Deoxyribonuclease Therapy Improves Stroke Outcomes in Aged Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Junxiang Yin,Michael Wu,Jennifer White,Ellie StClair,J Mocco,Michael F Waters

Nature communications 16:545 PubMed39788946

2025

The NE/AAT/CBG axis regulates adipose tissue glucocorticoid exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Luke D Boyle,Allende Miguelez-Crespo,Mhairi Paul,Elisa Villalobos,Julia N C Toews,Lisa Ivatt,Boglarka Nagy,Marisa Magennis,Natalie Z M Homer,Ruth Andrew,Victor Viau,Geoffrey L Hammond,Roland H Stimson,Brian R Walker,Mark Nixon

mBio 15:e0140924 PubMed38953359

2024

promotes inflammasome formation and NETosis during pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Steven G Sayson,Alan Ashbaugh,Aleksey Porollo,George Smulian,Melanie T Cushion

Immunity 57:52-67.e10 PubMed38091995

2023

trans-Endothelial neutrophil migration activates bactericidal function via Piezo1 mechanosensing.

Applications

Unspecified application

Species

Unspecified reactive species

Amitabha Mukhopadhyay,Yoshikazu Tsukasaki,Wan Ching Chan,Jonathan P Le,Man Long Kwok,Jian Zhou,Viswanathan Natarajan,Nima Mostafazadeh,Mark Maienschein-Cline,Ian Papautsky,Chinnaswamy Tiruppathi,Zhangli Peng,Jalees Rehman,Balaji Ganesh,Yulia Komarova,Asrar B Malik

Journal of virology 97:e0067423 PubMed37830821

2023

Adenoviral-vectored universal influenza vaccines administered intranasally reduce lung inflammatory responses upon viral challenge 15 months post-vaccination.

Applications

Unspecified application

Species

Unspecified reactive species

Julia A Misplon,Chia-Yun Lo,Torrie A Crabbs,Graeme E Price,Suzanne L Epstein

Nutrients 15: PubMed37630845

2023

Probiotic OMNi-BiOTiC 10 AAD Reduces Cyclophosphamide-Induced Inflammation and Adipose Tissue Wasting in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Beate Obermüller,Georg Singer,Bernhard Kienesberger,Barbara Mittl,Vanessa Stadlbauer,Angela Horvath,Wolfram Miekisch,Patricia Fuchs,Martina Schweiger,Laura Pajed,Holger Till,Christoph Castellani

International journal of molecular sciences 24: PubMed37175553

2023

Essential Involvement of Neutrophil Elastase in Acute Acetaminophen Hepatotoxicity Using BALB/c Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuko Ishida,Siying Zhang,Yumi Kuninaka,Akiko Ishigami,Mizuho Nosaka,Isui Harie,Akihiko Kimura,Naofumi Mukaida,Toshikazu Kondo

PLoS neglected tropical diseases 16:e0010224 PubMed35192633

2022

Neutrophil-dendritic cell interaction plays an important role in live attenuated Leishmania vaccine induced immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Parna Bhattacharya,Nevien Ismail,Ankit Saxena,Sreenivas Gannavaram,Ranadhir Dey,Timur Oljuskin,Adovi Akue,Kazuyo Takeda,James Yu,Subir Karmakar,Pradeep K Dagur,John Philip McCoy,Hira L Nakhasi

Nature aging 1:715-733 PubMed34514433

2021

Multi-omic profiling of primary mouse neutrophils predicts a pattern of sex and age-related functional regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan J Lu,Shalina Taylor,Kévin Contrepois,Minhoo Kim,Juan I Bravo,Mathew Ellenberger,Nirmal K Sampathkumar,Bérénice A Benayoun

British journal of pharmacology 178:4048-4068 PubMed34076894

2021

Neutrophil elastase promotes neointimal hyperplasia by targeting toll-like receptor 4 (TLR4)-NF-κB signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Yang,Qishan Chen,Li Mei,Guanmei Wen,Weiwei An,Xinmiao Zhou,Kaiyuan Niu,Chenxin Liu,Meixia Ren,Kun Sun,Qingzhong Xiao,Li Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Neutrophil Elastase ELISA Kit

ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (AB252356)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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