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AB270203

Mouse Nidogen-1 ELISA Kit (Entactin)

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Mouse Nidogen-1 ELISA Kit (Entactin) is a single-wash 90-min Simplestep used to quantify Mouse Nidogen-1 (Entactin) with a sensitivity of 44.15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ent, Nid1, Nidogen-1, NID-1, Entactin

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Sandwich ELISA - Mouse Nidogen-1 ELISA Kit (Entactin) (AB270203)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Nidogen-1 ELISA Kit (Entactin) (AB270203)

Example of mouse Nidogen-1 standard curve in Sample Diluent NS.

The Nidogen-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Nidogen-1 ELISA Kit (Entactin) (AB270203)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Nidogen-1 ELISA Kit (Entactin) (AB270203)

Interpolated concentrations of native Nidogen-1 in mouse serum and plasma samples.

The concentrations of Nidogen-1 were measured in duplicates, interpolated from the Nidogen-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 2.5%, plasma (heparin) 2.5%, and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nidogen-1 concentration was determined to be 59.56 ng/mL in serum, 165.19 ng/mL in plasma (citrate), 199.81 ng/ml in plasma (heparin), and 167.56 ng/ml in plasma (EDTA).

Sandwich ELISA - Mouse Nidogen-1 ELISA Kit (Entactin) (AB270203)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Nidogen-1 ELISA Kit (Entactin) (AB270203)

Interpolated concentrations of native Nidogen-1 in mouse heart tissue supernatant samples.

The concentrations of Nidogen-1 were measured in duplicates, interpolated from the Nidogen-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : heart supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nidogen-1 concentration was determined to be 27.66 ng/mL in heart supernatant.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 44.15 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB254325

Anti-Entactin/NID antibody [EPR22414-125]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (AB254325)

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Human Brevican ELISA Kit

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Proteins & Peptides

AB225643

Recombinant Mouse Entactin/NID protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Nidogen-1 ELISA Kit (Entactin) (ab270203) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nidogen-1 (Entactin) protein in cell culture media, cell culture supernatant, edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Nidogen-1 (Entactin) with 44.15 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Nidogen-1 is a glycoprotein found in the basement membrane. Nidogen-1 is a part of a network of proteins within the cell and is thought to be involved in cell-extracellular matrix interactions. Nidogen-1 is a ubiquitous and abundant protein, in contrast to nidogen-2 which has distinct expression patterns. Mice lacking both nidogens die shortly after birth due to lung and heart problems.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "105 - 110 %", "average": "= 107" }, { "sample": "Serum", "range": "81 - 103 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "89 - 97 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "96 - 98 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "87 - 100 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "83 - 87 %", "average": "= 85" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Entactin also known as nidogen-1 or NID1 is a significant glycoprotein of the extracellular matrix with a molecular mass of approximately 150 kDa. This protein is often referred to as nidogen and nidogen 1. It localizes mainly in basement membranes across various tissues. Entactin serves as an important structural component and mediator between other extracellular molecules contributing significantly to the maintenance and integrity of the basement membrane structure.
Biological function summary

Entactin interacts closely with laminin and type IV collagen within the extracellular matrix forming an important part of the basement membrane complex. This protein aids in the stabilization and linkage of these matrices playing an essential role in cellular adhesion and migration processes. By facilitating the structural assembly of basement membranes entactin supports cellular signaling pathways that are involved in tissue development and repair.

Pathways

Entactin acts within the integrin signaling pathway and the basement membrane formation pathway. It interacts with integrin receptors on the cell surface thereby influencing processes like cell adhesion and morphogenesis. Through these pathways entactin signals in coordination with laminins and collagens building a robust structural framework necessary for tissue integrity and function.

Entactin's disruption has links to cancer and certain genetic disorders affecting basement membrane integrity. Aberrant expression or dysfunction of entactin can contribute to the progression of tumor invasion due to its pivotal role in cellular adhesion. Furthermore it associates with muscular dystrophies through interactions with laminins and collagens highlighting its importance in maintaining normal tissue architecture and function.
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Product protocols

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Target data

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
See full target information Nid1
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websiteProtocolBooklet
en
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Product promise

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