AB279417

Mouse NLRP3 ELISA Kit

Recombinant
SimpleStep
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(7 Publications)

Mouse NLRP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse NLRP3 with a sensitivity of 0.57 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Cias1, Mmig1, Nalp3, Pypaf1, Nlrp3, Cold autoinflammatory syndrome 1 protein homolog, Cryopyrin, Mast cell maturation-associated-inducible protein 1, PYRIN-containing APAF1-like protein 1

3 Images

ELISA - Mouse NLRP3 ELISA Kit (AB279417)

ELISA

Supplier Data

ELISA - Mouse NLRP3 ELISA Kit (AB279417)

sELISA

Supplier Data

Sandwich ELISA - Mouse NLRP3 ELISA Kit (AB279417)

Interpolated concentrations of native NLRP3 in mouse J774A.1 stimulated and J774A.1 unstimulated cell extract based on a 500 μg/mL extract load.

The concentrations of NLRP3 were measured in duplicate and interpolated from the NLRP3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NLRP3 concentration was determined to be 187.6 ng/mL in J774A.1 stimulated cell extract and 5.45 ng/mL in J774A.1 unstimulated cell extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse NLRP3 ELISA Kit (AB279417)

Example of mouse NLRP3 standard curve in 1X Cell Extraction Buffer.

The NLRP3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.57 ng/mL

Range

3.91 - 250 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse NLRP3 ELISA Kit ab279417 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse NLRP3 in cell lysate, tissue extracts. This SimpleStep sensitivity is 0.57 ng/mL.

How the assay works

Mouse NLRP3 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse NLRP3 ELISA Kit ab279417 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-NLRP3 antibody [EPR23073-75] - BSA and Azide free (Capture) ab281559
- Anti-NLRP3 antibody [EPR23073-59] - BSA and Azide free (Detector) ab281559

NACHT, LRR and PYD domains-containing protein 3 (NLRP3), encoded by the NLRP3 gene, is known to have a vital role in the innate immunity and inflammation. NLRP3 is highly expressed in macrophages and as a component of the inflammasomes in which it functions to detect products of damaged cells and subsequently activates itself to trigger an immune response. Mutations in the NLRP3 has been shown in studies to be associated with a wide spectrum of dominantly inherited autoimmune diseases. The standard protein provided in ab279417 is the N-terminal 215 amino acids fragment of Mouse NLRP3.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "111 - 118 %", "average": "= 115" }, { "sample": "Cell Lysate", "range": "99 - 117 %", "average": "= 106" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NLRP3 also known as NALP3 or cryopyrin is a protein important for the inflammatory response. It is a component of the inflammasome which senses pathogenic microorganisms and stress. NLRP3 weighs around 118 kDa and is widely expressed in innate immune cells particularly macrophages and dendritic cells. Researchers often use techniques such as 'NLRP3 WB' (Western blot) and 'NLRP3 immunofluorescence' to study NLRP3 expression and localization.

Biological function summary

NLRP3 is central to the body’s defense mechanism by participating in forming the inflammasome complex which activates caspase-1. This activation leads to the cleavage of pro-inflammatory cytokines like IL-1? and IL-18 into their active forms. NLRP3's function is essential for mounting an effective immune response to infections and damage signals. It senses a range of stimuli including crystalline substances and cellular stress triggering an immune response. Its expression is tightly regulated to prevent excessive inflammation.

Pathways

The NLRP3 inflammasome is key in the innate immune response and inflammation pathways. It links cellular stress signals to inflammation through the activation of caspase-1 which is important for the IL-1 signaling pathway. NLRP3 interacts with other proteins like ASC (apoptosis-associated speck-like protein containing a CARD) to form the inflammasome complex which is a core part of the inflammatory signaling pathways and links innate and adaptive immunity.

NLRP3 has a significant role in inflammatory diseases and is linked to conditions like cryopyrin-associated periodic syndromes (CAPS) and gout. Mutations or dysregulation of NLRP3 lead to hyperactivation resulting in autoimmune and autoinflammatory conditions. NLRP3's interaction with IL-1 contributes to the excessive inflammatory response seen in these disorders. Understanding NLRP3's function in these pathways can inform therapeutic approaches targeting chronic inflammatory diseases.

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Product protocols

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Target data

Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis (PubMed : 19362020, PubMed : 23582325, PubMed : 26642356, PubMed : 26814970, PubMed : 27374331, PubMed : 27929086, PubMed : 28656979, PubMed : 28847925, PubMed : 30518920, PubMed : 36178239). In response to pathogens and other damage-associated signals that affect the integrity of membranes, initiates the formation of the inflammasome polymeric complex composed of NLRP3, CASP1 and PYCARD/ASC (PubMed : 16407889, PubMed : 18403674, PubMed : 19362020, PubMed : 26642356, PubMed : 26814970, PubMed : 27374331, PubMed : 28847925). Recruitment of pro-caspase-1 (proCASP1) to the NLRP3 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion and pyroptosis (PubMed : 16546100, PubMed : 17008311, PubMed : 26642356, PubMed : 26814970, PubMed : 27374331, PubMed : 28847925). Activation of NLRP3 inflammasome is also required for HMGB1 secretion; stimulating inflammatory responses (PubMed : 22801494). Under resting conditions, ADP-bound NLRP3 is autoinhibited (By similarity). NLRP3 activation stimuli include extracellular ATP, nigericin, reactive oxygen species, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, such as asbestos, silica, aluminum salts, cytosolic dsRNA, etc (PubMed : 16407888, PubMed : 16407889, PubMed : 16407890, PubMed : 18403674, PubMed : 19362020, PubMed : 37001519). Almost all stimuli trigger intracellular K(+) efflux (PubMed : 23809161). These stimuli lead to membrane perturbation and activation of NLRP3 (By similarity). Upon activation, NLRP3 is transported to microtubule organizing center (MTOC), where it is unlocked by NEK7, leading to its relocalization to dispersed trans-Golgi network (dTGN) vesicle membranes and formation of an active inflammasome complex (PubMed : 26814970, PubMed : 34615873, PubMed : 34861190). Associates with dTGN vesicle membranes by binding to phosphatidylinositol 4-phosphate (PtdIns4P) (PubMed : 30487600). Shows ATPase activity (PubMed : 34861190).. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (PubMed : 26098997). During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription (PubMed : 26098997). Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3' (PubMed : 26098997). May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PubMed : 26098997).

See full target information Nlrp3

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:656 PubMed37813835

2023

Polyphenol-rich diet mediates interplay between macrophage-neutrophil and gut microbiota to alleviate intestinal inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Han,Yujun Wu,Dongdong Lu,Jiaman Pang,Jie Hu,Xiangyu Zhang,Zhenyu Wang,Guolong Zhang,Junjun Wang

Immunity, inflammation and disease 11:e830 PubMed37102650

2023

Acinetobacter baumannii outer membrane protein A induces autophagy in bone marrow-derived dendritic cells involving the PI3K/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyi Tan,Liyan Cao

Frontiers in immunology 14:1165602 PubMed37077909

2023

Loss of NLRP3 reduces oxidative stress and polarizes intratumor macrophages to attenuate immune attack on endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Zhu,Yanli Xu,Juan Wang,Zhuowei Xue,Tian Qiu,Jing Chen

Frontiers in immunology 13:1064303 PubMed36505450

2022

Persistent expression of NLRP3 in spinal microglia promotes development of lumbar disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Jing Zhang

ACS omega 7:41627-41640 PubMed36406541

2022

Anchoring of Nanocomposites Based on Novel Metal Nanocomplexes/Nanocarbonaceous Surfaces and Assessing Their In Vivo Anticancer Effects on Ehrlich Ascites Tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Heba Bassiony Ghanem,Rehab Galal El-Sharkawy,Rania Hosny Taha

Frontiers in immunology 13:1057746 PubMed36405726

2022

NLRP3+ macrophages aggravate inflammatory cystitis in diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Yubing Peng,Yan Gao

Pharmaceuticals (Basel, Switzerland) 15: PubMed35631426

2022

Perindopril/Ambrosin Combination Mitigates Dextran Sulfate Sodium-Induced Colitis in Mice: Crosstalk between Toll-Like Receptor 4, the Pro-Inflammatory Pathways, and SIRT1/PPAR-γ Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Kabel,Aliaa Atef,Hany M Borg,Azza A K El-Sheikh,Hana J Al Khabbaz,Hany H Arab,Remon S Estfanous

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse NLRP3 ELISA Kit

ELISA - Mouse NLRP3 ELISA Kit (AB279417)

Sandwich ELISA - Mouse NLRP3 ELISA Kit (AB279417)

Sandwich ELISA - Mouse NLRP3 ELISA Kit (AB279417)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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