Mouse NMDAR1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse NMDAR1 with a sensitivity of 27.74 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 203.13 - 13000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 27.74 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:12008020, PubMed:12860921, PubMed:14602821, PubMed:1532151, PubMed:8060614, PubMed:9049108). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP) (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:12008020, PubMed:1532151, PubMed:7790891, PubMed:8060614). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (PubMed:12860921). Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:12008020, PubMed:14602821).
Alternative names
Glurz1, Grin1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1
What's included?
Recommended products
Mouse NMDAR1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse NMDAR1 with a sensitivity of 27.74 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 203.13 - 13000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 27.74 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 2.9 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 3.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 92 | Range 80 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse NMDAR1 ELISA kit (ab282862) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse NMDAR1 protein in mouse cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NMDAR1 with 27.74 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse NMDA receptor (NMDAR1) is a and protein receptor activated upon and glutamate binding. NMDAR1 is a component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. NMDA receptor complex channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition. Mouse NMDAR1 shares 99.8% and 99.3% sequence homology with rat and human, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NMDAR1 also known as GluN1 is a subunit of the NMDA receptor a type of ion channel protein found in the brain. This receptor weighs around 120 kDa and plays an important role in synaptic transmission and plasticity. NMDAR1 operates as part of a receptor complex that allows for calcium influx when activated. It shows high expression throughout the central nervous system primarily in neurons across the cortex and hippocampus. The receptor's function is important to certain calcium-dependent processes in the brain.
- Biological function summary
NMDA receptors including the NMDAR1 subunit serve essential functions in synaptic plasticity and memory processes. As part of the receptor complex NMDAR1 contributes to the control of synaptic strength. It requires co-activation by glutamate and glycine or D-serine linking its function to excitatory neurotransmission. The receptor's capacity to modulate synaptic connections forms the basis of learning and long-term memory.
- Pathways
NMDAR1 plays a critical role in the glutamatergic pathway influencing synaptic plasticity and memory function. It interacts with other proteins like PSD-95 within the postsynaptic density. NMDAR1 is also involved in the calcium signaling pathway due to its ability to facilitate calcium entry into neurons upon synaptic activity. This positions the receptor as an important modulator in pathways that control neuronal communication and plasticity.
- Associated diseases and disorders
NMDA receptor dysfunction including the NMDAR1 subunit links to neurodegenerative diseases such as Alzheimer's. The alterations in NMDAR1 function can lead to excitotoxicity contributing to neuronal damage. NMDAR1 also associates with schizophrenia where altered glutamatergic transmission has been observed. Here abnormalities in proteins like the synaptic scaffolding protein PSD-95 have also been noted to relate to NMDAR1 dysfunction in the pathophysiology of these disorders.
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2 product images
Sandwich ELISA - Mouse NMDAR1 ELISA Kit (ab282862)
Example of mouse NMDAR1 standard curve in 1X Cell Extraction Buffer PTR.
The NMDAR1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse NMDAR1 ELISA Kit (ab282862)
Interpolated concentrations of native NMDAR1 in mouse brain tissue extract based on a 125 μg/mL extract load.
The concentrations of NMDAR1 were measured in duplicate and interpolated from the NMDAR1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NMDAR1 concentration was determined to be 7515.05 pg/mL in brain tissue extract.
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