Mouse NOV ELISA Kit (CCN3) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse NOV (CCN3) in Mouse in Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant samples.
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- Medical science monitor : international medical jo 25:6755-67662019The Predictive Role of Tenascin-C and Cellular Communication Network Factor 3 (CCN3) in Post Hepatectomy Liver Failure in a Rat Model and 50 Patients Following Partial Hepatectomy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Li et. al.PubMed 31494663
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 130 - 8000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 51 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Immediate-early protein playing a role in various cellular processes including proliferation, adhesion, migration, differentiation and survival. Acts by binding to integrins or membrane receptors such as NOTCH1. Essential regulator of hematopoietic stem and progenitor cell function. Inhibits myogenic differentiation through the activation of Notch-signaling pathway. Inhibits vascular smooth muscle cells proliferation by increasing expression of cell-cycle regulators such as CDKN2B or CDKN1A independently of TGFB1 signaling. Ligand of integrins ITGAV:ITGB3 and ITGA5:ITGB1, acts directly upon endothelial cells to stimulate pro-angiogenic activities and induces angiogenesis. In endothelial cells, supports cell adhesion, induces directed cell migration (chemotaxis) and promotes cell survival. Also plays a role in cutaneous wound healing acting as integrin receptor ligand. Supports skin fibroblast adhesion through ITGA5:ITGB1 and ITGA6:ITGB1 and induces fibroblast chemotaxis through ITGAV:ITGB5. Seems to enhance bFGF-induced DNA synthesis in fibroblasts (By similarity). Involved in bone regeneration as a negative regulator (PubMed:23653360). Enhances the articular chondrocytic phenotype, whereas it repressed the one representing endochondral ossification (By similarity). Impairs pancreatic beta-cell function, inhibits beta-cell proliferation and insulin secretion (PubMed:23705021). Plays a role as negative regulator of endothelial pro-inflammatory activation reducing monocyte adhesion, its anti-inflammatory effects occur secondary to the inhibition of NF-kappaB signaling pathway (By similarity). Contributes to the control and coordination of inflammatory processes in atherosclerosis (PubMed:24722330). Attenuates inflammatory pain through regulation of IL1B- and TNF-induced MMP9, MMP2 and CCL2 expression. Inhibits MMP9 expression through ITGB1 engagement (By similarity). Brain osteoanabolic hormone (PubMed:38987585). During lactation, maintains the maternal skeleton and viability of offspring (PubMed:38987585). In this context, may act on osteochondral skeletal stem cells (PubMed:38987585).
Alternative names
Nov, Ccn3, CCN family member 3, Cellular communication network factor 3, Nephroblastoma-overexpressed gene protein homolog, Protein NOV homolog, NovH
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Mouse NOV ELISA Kit (CCN3) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse NOV (CCN3) in Mouse in Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 130 - 8000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 51 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 8.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 5.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 111 | Range 107 - 116 % |
Sample type Citrate plasma | Average % = 104 | Range 103 - 106 % |
Sample type EDTA Plasma | Average % = 88 | Range 86 - 88 % |
Sample type Heparin Plasma | Average % = 85 | Range 85 - 85 % |
Sample type Mouse brain extract | Average % = 113 | Range 108 - 117 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse NOV ELISA Kit (CCN3) (ab205570) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NOV (CCN3) protein in cell culture supernatant, tissue extracts, plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NOV (CCN3) with 51 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
NOV is a member of the CCN family of extracellular matrix associated signaling proteins. As a secreted protein, NOV regulates cell survival, adhesion, proliferation, differentiation and migration. In humans, NOV expression is associated with metastasis and poor prognosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCN3 also known as NOV (Nephroblastoma Overexpressed) is a protein that has important roles in cellular processes. This protein belongs to the CCN family of proteins with an approximate molecular mass of 34-38 kDa. CCN3 is expressed in various tissues including kidney heart and brain reflecting its involvement in diverse physiological activities. It is particularly noted for its role in tissue development and repair processes.
- Biological function summary
CCN3 influences several aspects of cell behavior including proliferation differentiation and migration. It does not function as part of a larger complex but acts as an individual entity facilitating communication between cells and the extracellular matrix. CCN3 also affects angiogenesis a critical process in both health and disease by modulating vascular endothelial growth factor (VEGF) pathways therefore impacting blood vessel formation and maintenance.
- Pathways
CCN3 interacts primarily with key signaling pathways such as Wnt and TGF-beta signaling. Within the Wnt pathway CCN3 serves as a regulatory component modulating the activity of other proteins involved in cell growth. Similarly in the TGF-beta pathway it contributes to the regulation of cellular proliferation and differentiation by affecting interactions with other proteins like SMADs which are signal transducers in TGF-beta signaling.
- Associated diseases and disorders
CCN3 has connections to conditions like nephroblastoma often referred to as Wilms Tumor and fibrosis. In nephroblastoma altered expression of CCN3 correlates with tumor progression and metastasis. It interacts with proteins like WNT1 influencing tumor growth and development. In fibrosis which affects organs such as the liver and lungs CCN3’s modulation of TGF-beta signaling plays a significant role impacting fibrogenesis through its interaction with ECM components.
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5 product images
Sandwich ELISA - Mouse NOV ELISA Kit (CCN3) (ab205570)
Example of mouse NOV standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse NOV ELISA Kit (CCN3) (ab205570)
Example of mouse NOV standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse NOV ELISA Kit (CCN3) (ab205570)
Example of mouse NOV standard curve in Sample Diluent 75BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse NOV ELISA Kit (CCN3) (ab205570)
Linearity of dilution of native mouse NOV protein in mouse tissue extracts.
The concentrations of mouse NOV were interpolated from the mouse NOV standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted. Interpolated values of NOV are plotted based on an extract load of 250 μg/mL.
Sandwich ELISA - Mouse NOV ELISA Kit (CCN3) (ab205570)
Mouse NOV expression is shown for plasma samples.
Native NOV was measured in duplicate in 50% EDTA, heparin and citrate plasma diluted in Sample Diluent 75BS and concentrations interpolated from a standard curve diluted in Sample Diluent 75BS. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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