AB233626

Mouse NSE / Neuron-specific Enolase ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Mouse NSE / Neuron-specific Enolase ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse NSE / Neuron-specific Enolase with a sensitivity of 50.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Eno-2, Eno2, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE

3 Images

Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (AB233626)

sELISA

Supplier Data

Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (AB233626)

Example of mouse Neuron-specific Enolase standard curve in 1X Cell Extraction Buffer PTR.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (AB233626)

Example of mouse Neuron-specific Enolase standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (AB233626)

Interpolated concentrations of native Neuron-specific Enolase in mouse brain tissue extract based on a 100 μg/mL extract load and Neuro-2a cell extract based on a 250 μg/mL extract load.

The concentrations of Neuron-specific Enolase were measured in duplicate and interpolated from the Neuron-specific Enolase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be 40,511 pg/mL in mouse brain tissue extract and 8,858 pg/mL in Neuro-2a cell extract.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 50.8 pg/mL

Range

312.5 - 60000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NSE / Neuron-specific Enolase protein in tissue extracts, cell culture supernatant, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NSE / Neuron-specific Enolase with 50.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Brain", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Intra", "sample": "Mouse Brain", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" } ]

style

left-column

Recovery

[ { "sample": "Cell culture extracts", "range": "97 - 107 %", "average": "= 101" }, { "sample": "Tissue Extracts", "range": "113 - 118 %", "average": "= 115" }, { "sample": "Cell culture media", "range": "92 - 98 %", "average": "= 89" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233626-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233626-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233626-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB233626-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Neuron-specific Enolase Lyophilized Recombinant Protein", "number":"AB233626-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Neuron-specific Enolase Capture Antibody", "number":"AB233626-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233626-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233626-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB233626-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233626-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233626-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Neuron-specific Enolase Detector Antibody", "number":"AB233626-CMP07", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.

Biological function summary

NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.

Pathways

NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.

NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.

style

left-column

Product protocols

style

left-column

Target data

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

See full target information Eno2

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental zoology. Part A, Ecological and integrative physiology 341:293-306 PubMed38229259

2024

Investigating the role of Purkinje fibers and synaptic connectivity in balance regulation through comprehensive ultrastructural and immunohistochemical analysis of the donkey's (Equus asinus) cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Samar M Ez Elarab,Mohamed A M Alsafy,Samir A A El-Gendy,Neveen E R El-Bakary,Ahmed Elsayed Noreldin,Ahmed M Rashwan

Translational neuroscience 11:371-379 PubMed33335777

2020

EGCG treats ICH via up-regulating miR-137-3p and inhibiting Parthanatos.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjun Wang,Xuejun Kuang,Zhao Peng,Conghui Li,Chengwu Guo,Xi Fu,Junhong Wu,Yang Luo,Xiaolin Rao,Xiangjuan Zhou,Bin Huang,Weijun Tang,Yinjuan Tang

The Analyst 145:4164-4172 PubMed32369047

2020

In situ amplified photothermal immunoassay for neuron-specific enolase with enhanced sensitivity using Prussian blue nanoparticle-loaded liposomes.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Juan Zhi,Ai-Li Sun,Dianping Tang

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse NSE / Neuron-specific Enolase ELISA Kit

Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (AB233626)

Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (AB233626)

Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (AB233626)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Secondary Antibodies

Assay Kits

Assay Kits

Assay Kits

Product promise