Mouse Nucleophosmin ELISA Kit
- Recombinant
- SimpleStep
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Mouse Nucleophosmin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Nucleophosmin with a sensitivity of 600 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, Npm1
- sELISA
Supplier Data
Sandwich ELISA - Mouse Nucleophosmin ELISA Kit (AB216172)
Example of mouse Nucleophosmin standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Nucleophosmin ELISA Kit (AB216172)
Example of mouse Nucleophosmin standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Nucleophosmin ELISA Kit (AB216172)
Interpolated concentrations of native Nucleophosmin in mouse RAW 264.7 cell extract and C6 cell extract samples based on 250 μg/mL and 500 μg/mL extract loads, respectively.
The concentrations of Nucleophosmin were measured in duplicate and interpolated from the Nucleophosmin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nucleophosmin concentration was determined to be 57.49 ng/mL in RAW 264.7 cell extract and 100.26 ng/mL in C6 cell extract.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Nucleophosmin ELISA Kit (AB216172)
Interpolated concentrations of native Nucleophosmin in mouse serum and plasma samples.
The concentrations of Nucleophosmin were measured in duplicates, interpolated from the Nucleophosmin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nucleophosmin concentration was determined to be 21.07 ng/mL in neat serum, 26.17 ng/mL in neat plasma (citrate), 21.49 ng/mL in neat plasma (EDTA), and 44.43 ng/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Mouse Nucleophosmin ELISA Kit (AB216172)
Interpolated concentrations of native Nucleophosmin in mouse cell culture supernatant samples.
The concentrations of Nucleophosmin were measured in duplicates, interpolated from the Nucleophosmin standard curves and corrected for sample dilution. Undiluted samples are as follows : RAW 264.7 media 2.5%, L929 media 5%, and J774A.1 media 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nucleophosmin concentration was determined to be 2,767.43 ng/mL in neat RAW 264.7 media, 1,790.80 ng/mL in neat L929 media, and 889.01 ng/mL in neat J774A.1 media. RAW 264.7 media was cultured in HGDMEM media with kanamycin and L-glutamine for 24 hours (serum free) and then for another 48 hours with 1% PHA. J774A.1 media was cultured for 72 hours in HGDMEM media with kanamycin, 10% fetal bovine serum, and 1.5% PHA plus 10 ng/mL PMA. L929 media was cultured for 72 hours in MEM media with kanamycin, 10% horse serum, and 1.5% PHA plus 10 ng/mL PMA.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Nucleophosmin ELISA Kit (AB216172)
Interpolated concentrations of native Nucleophosmin in unstimulated and stimulated L929 cultured media.
The concentrations of Nucleophosmin were measured in duplicates, interpolated from the Nucleophosmin standard curves and corrected for sample dilution. Undiluted samples are as follows : L929 unstimulated media 5% and L929 stimulated media 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nucleophosmin concentration was determined to be 3.12 ng/mL in neat unstimulated L929 media, and 92.60 ng/mL in neat stimulated L929 media. L929 media was cultured for 72 hours in MEM media with kanamycin and 10% horse serum without (unstimulated) and with (stimulated) 1.5% PHA plus 10 ng/mL PMA.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Nucleophosmin ELISA Kit (AB216172)
Interpolated concentrations of spiked Nucleophosmin in mouse serum and plasma samples.
The concentrations of Nucleophosmin were measured in duplicates, interpolated from the Nucleophosmin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nucleophosmin concentration was determined to be 134.31 ng/mL in neat serum, 138.54 ng/mL in neat plasma (citrate), 138.47 ng/mL in neat plasma (EDTA), and 150.88 ng/mL in neat plasma (heparin).
Reactivity data
Product details
Mouse Nucleophosmin ELISA Kit (ab216172) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nucleophosmin protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Nucleophosmin with 600 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse Nucleophosmin is involved in an array of diverse cellular processes, including ribosome biogenesis, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. In concert with BRCA2, Nucleophosmin also regulates centrosome duplication. Mouse Nucleophosmin is 292 aa in length and shares 99% and 95% sequence homology with rat and human Nucleophosmin, respectively.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
One of the central roles of NPM1 is its involvement in the regulation of cell growth and proliferation. NPM1 forms a complex with other nucleolar proteins affecting ribosome subunit assembly and transport. This protein also participates in the control of the ARF/p53 pathway by sequestering p53-regulating proteins which are essential for cell cycle control and apoptosis. Its interactions with proteins such as nucleolin and nucleophosmin-like proteins allow NPM1 to perform its functions in organizing the nucleolus and regulating responses to stress signals.
Pathways
The NPM1 protein is integrally involved in key cellular pathways like the regulation of the cell cycle and apoptosis. It directly interacts with pathways such as the ribosome biogenesis pathway and the p53 pathway. NPM1 has connections with proteins like ARF and MDM2 which play roles in retinoblastoma protein (pRb) regulation and in the control of tumor-suppressor activities. These pathways under NPM1’s influence are critical for maintaining cellular homeostasis and ensuring proper cell cycle checkpoints.
Product protocols
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Target data
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