AB208346

Mouse OB Cadherin ELISA Kit

Recombinant
SimpleStep
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Mouse OB Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse OB Cadherin with a sensitivity of 7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Cad-11, Cdh11, Cadherin-11, OSF-4, Osteoblast cadherin, OB-cadherin

3 Images

Sandwich ELISA - Mouse OB Cadherin ELISA Kit (AB208346)

sELISA

Supplier Data

Sandwich ELISA - Mouse OB Cadherin ELISA Kit (AB208346)

Interpolated concentrations of native OB-Cadherin in mouse plasma (heparin) and urine samples.

The concentrations of OB Cadherin were measured in duplicates, interpolated from the OB Cadherin standard curves and corrected for sample dilution. 1X diluted samples are as follows : plasma (heparin) 25%, and urine 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OB Cadherin concentration was determined to be 1,343 pg/mL in plasma (heparin) and 1,000 pg/mL in urine.

sELISA

Supplier Data

Sandwich ELISA - Mouse OB Cadherin ELISA Kit (AB208346)

Example of mouse OB Cadherin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse OB Cadherin ELISA Kit (AB208346)

Interpolated concentrations of native OB-Cadherin in mouse serum, plasma (citrate) and plasma (EDTA) samples.

The concentrations of OB Cadherin were measured in duplicates, interpolated from the OB Cadherin standard curves and corrected for sample dilution. 1X diluted samples are as follows : serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OB Cadherin concentration was determined to be 3,771 pg/mL in serum, 827 pg/mL in plasma (citrate) and 1,172 pg/mL in plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 7 pg/mL

Range

46.9 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse OB Cadherin ELISA Kit (ab208346) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OB Cadherin protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, urine, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse OB Cadherin with 7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

OB Cadherin (also known as Cadherin-11) is a member of the Cadherin family of calcium-dependent cell adhesion proteins. OB Cadherin is a single-pass transmembrane protein on the plasma membrane. The extracellular portion contains 5 cadherin domains that bind calcium and form homodimeric interactions.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

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Recovery

[ { "sample": "Serum", "range": "109 - 115 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "76 - 94 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "101 - 109 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "106 - 125 %", "average": "= 114" }, { "sample": "Heparin Plasma", "range": "112 - 122 %", "average": "= 116" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OB Cadherin is a calcium-dependent cell adhesion molecule also known as Cadherin-11. It has a mass of about 120 kDa. OB Cadherin is expressed widely in mesenchymal tissues including osteoblasts fibroblasts and synovial tissues. This cadherin plays a role in the regulation of cell-cell adhesion influencing the mechanical structure and integrity of tissues. Its expression in differentiated cells indicates its involvement in tissue maintenance and repair. OB Cadherin is also contributing to the segregation of different cell types during developmental processes.

Biological function summary

OB Cadherin plays an important role in tissue morphogenesis and remodeling. It is a part of the adherens junction complex which also includes catenins. This complex mediates cell contact and communication essential for structural integrity and signaling. OB Cadherin supports the proper organization and separation of cells within tissues impacting processes like bone development and wound healing. It acts as a mediator in mechanical signaling influencing cellular responses to mechanical stress.

Pathways

OB Cadherin participates in signaling pathways involved in cell adhesion and migration notably in the Wnt and BMP pathways. These pathways are essential for developmental processes and maintain tissue homeostasis. OB Cadherin interacts closely with proteins like beta-catenin playing a role in transcriptional regulation that influences cell fate decisions. Its function in these pathways demonstrates its critical role in regulating cellular activities tied to growth and differentiation.

OB Cadherin is linked to conditions like rheumatoid arthritis and cancer metastasis. In rheumatoid arthritis overexpression of OB Cadherin in synovial tissues can contribute to joint inflammation and tissue destruction associated with interactions with fibroblast-like synoviocytes. In cancer metastasis OB Cadherin's role in cell adhesion and migration highlights its potential influence in the spread of cancer cells often in relation to altered interactions with proteins like N-cadherin. These associations make OB Cadherin an important target for research into disease mechanisms and therapies.

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Product protocols

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Required for proper focal adhesion assembly. Involved in the regulation of cell migration.

See full target information Cdh11

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse OB Cadherin ELISA Kit

Sandwich ELISA - Mouse OB Cadherin ELISA Kit (AB208346)

Sandwich ELISA - Mouse OB Cadherin ELISA Kit (AB208346)

Sandwich ELISA - Mouse OB Cadherin ELISA Kit (AB208346)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

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Proteins & Peptides

Proteins & Peptides

Product promise