Mouse OGA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse OGA with a sensitivity of 0.063 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.23 - 15 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.063 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cleaves GlcNAc but not GalNAc from O-glycosylated proteins (PubMed:16517082). Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1 (By similarity). Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:16517082). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (By similarity).
Alternative names
Hexc, Kiaa0679, Mgea5, Oga, Protein O-GlcNAcase, OGA, Beta-N-acetylhexosaminidase, Beta-hexosaminidase, Bifunctional protein NCOAT, Meningioma-expressed antigen 5, N-acetyl-beta-D-glucosaminidase, N-acetyl-beta-glucosaminidase
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Mouse OGA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse OGA with a sensitivity of 0.063 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.23 - 15 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.063 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 6.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 8.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 98 | Range 86 - 108 % |
Sample type Tissue Extracts | Average % = 117 | Range 108 - 124 % |
Sample type Cell Lysate | Average % = 100 | Range 98 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse OGA ELISA Kit (ab255726) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OGA protein in tissue extracts, tissue lysate, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse OGA with 0.063 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Protein O-GlcNAcase (OGA), also referred to as Beta-N-Acetylhexosaminidase and Meningioma-expressed antigen 5 (MGEA5) is a 916 amino acid enzyme encoded by the gene *OGA*. OGA catalyzes the hydrolysis of GlcNac (N-acetyl-Beta-D-glucosamine) from O-glycosylated serine or threonine residues to form free N-acetyl-D-glucosamine generally in the cytosol. Thiazoline derivatives of GlcNAc have been found to act as OGA enzyme inhibitors by mimicking the reaction intermediates. OGA has a broad range of proteins that act as substrates, which may modulate downstream effects such as transcription and proteomic activities. OGA plays a role in embryonic development, brain activity, and hormone production. Mouse OGA protein shares a 98 and 97% sequence identity with rat and human protein, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MGEA5 also referred to as OGA or O-GlcNAcase serves an important mechanical role in cellular metabolism. This enzyme with a molecular mass of around 103 kDa is primarily involved in the removal of O-GlcNAc modifications from serine/threonine residues on nuclear and cytoplasmic proteins. It is expressed in various tissues but exhibits predominant activity in the brain liver and pancreas. Alternative names like "OGA stains" or "OGA b" may also be encountered in literature or research contexts.
- Biological function summary
MGEA5 acts as an important modulator of protein function and regulation by cycling O-GlcNAc modifications thereby influencing processes like transcription signal transduction and apoptosis. While not part of a well-defined complex it frequently interacts with other proteins involved in glucose metabolism and the cellular stress response. Its activity works in concert with OGT (O-GlcNAc transferase) which adds O-GlcNAc maintaining a balance important for cellular function.
- Pathways
MGEA5 involvement centers largely around glucose homeostasis and the insulin signaling pathway. It modulates the function of proteins like insulin receptor substrate (IRS) and other key regulators in this pathway affecting metabolic regulation and energy homeostasis. It shows a direct relationship with OGT as they reciprocate in action effects highlighting their collaborative role in cellular pathways that affect cellular signaling and survival.
- Associated diseases and disorders
MGEA5 has been implicated in neurodegenerative diseases and diabetes. In Alzheimer's disease altered O-GlcNAc cycling affects tau protein homeostasis which is significant in disease pathogenesis. Likewise in diabetes MGEA5 influences insulin resistance by regulating O-GlcNAc levels on insulin signaling molecules. Dysregulation of these processes may also involve proteins such as beta-amyloid further linking MGEA5 to these pathological states.
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2 product images
Sandwich ELISA - Mouse OGA ELISA Kit (ab255726)
Example of mouse OGA standard curve in 1X Cell Extraction Buffer PTR.
The OGA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse OGA ELISA Kit (ab255726)
Interpolated concentrations of native OGA in mouse brain tissue extract, rat C6 cell extract, and human SH-SY5Y cell extract samples.
The concentrations of OGA were measured in duplicates, interpolated from the OGA standard curves and corrected for sample dilution. Undiluted samples are as follows mouse brain tissue extract, 1,250 μg/mL, rat C6 cell extract, 188 μg/mL, and human SH-SY5Y 300 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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