Mouse Oncostatin M ELISA Kit (OSM) is a single-wash 90-min Simplestep used to quantify Mouse Oncostatin M (OSM) with a sensitivity of 4.36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 11.71875 - 750 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.36 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity). Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity).
Alternative names
Oncostatin-M, OSM, Osm
What's included?
Recommended products
Mouse Oncostatin M ELISA Kit (OSM) is a single-wash 90-min Simplestep used to quantify Mouse Oncostatin M (OSM) with a sensitivity of 4.36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 11.71875 - 750 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.36 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 99 | Range 93 - 101 % |
Sample type Serum | Average % = 87 | Range 86 - 88 % |
Sample type EDTA Plasma | Average % = 87 | Range 85 - 89 % |
Sample type Heparin Plasma | Average % = 87 | Range 83 - 94 % |
Sample type Citrate plasma | Average % = 86 | Range 79 - 91 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Oncostatin M ELISA Kit (OSM) (ab263891) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Oncostatin M (OSM) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Oncostatin M (OSM) with 4.36 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Oncostatin M (OSM) is a growth regulator. Oncostatin M inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Oncostatin M uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). It is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. Rat Oncostatin M is 73% identical to mouse Oncostatin M.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oncostatin M (OSM) also known as Oncostatin M protein or OSM protein is a cytokine with significant roles in inflammation and hematopoiesis. This protein weighs around 28 kDa and is expressed mainly in activated T cells monocytes and macrophages. It interacts with specific receptors on the cell surface to transmit signals that regulate cell growth and function. Oncostatin M is part of the interleukin-6 (IL-6) family contributing to various cellular processes.
- Biological function summary
Oncostatin M modulates a range of cellular activities including cell proliferation differentiation and apoptosis. This cytokine affects diverse cell types such as fibroblasts endothelial cells and epithelial cells showing particular influence in processes like tissue remodeling and immune response. It acts as a monomer and does not need assembly into a larger complex to exert its function. Its ability to trigger gene expression differentiates it from other closely related cytokines.
- Pathways
Oncostatin M involves itself in significant pathways like the JAK/STAT signaling pathway and the MAPK pathway. Through these pathways it promotes signal transduction essential for immune regulation and cellular response to external stimuli. OSM interacts with proteins like the gp130 receptor a common signaling component for cytokine receptors in its family and influences downstream effectors involved in these pathways.
- Associated diseases and disorders
Oncostatin M associates itself with inflammatory diseases such as rheumatoid arthritis and cardiovascular disorders. Its elevated levels in pathological conditions highlight its role in exacerbating inflammatory responses. In rheumatoid arthritis OSM promotes synovial inflammation while in cardiovascular diseases it may influence vascular remodeling. Connections to inflammatory proteins such as IL-6 further highlight its part in driving disease pathogenesis making it an intriguing target for therapeutic intervention.
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3 product images
Sandwich ELISA - Mouse Oncostatin M ELISA Kit (OSM) (ab263891)
Mouse Oncostatin M standard curve comparison.
Standard Curve comparison between Mouse Oncostatin M SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
Sandwich ELISA - Mouse Oncostatin M ELISA Kit (OSM) (ab263891)
Example of mouse Oncostatin M standard curve in Sample Diluent NS.
The Oncostatin M standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Oncostatin M ELISA Kit (OSM) (ab263891)
Interpolated concentrations of native Oncostatin M in mouse P388D1 cell culture supernatant and mouse spleen cell culture supernatant samples.
The concentrations of Oncostatin M were measured in duplicates, interpolated from the Oncostatin M standard curves and corrected for sample dilution. Undiluted samples are as follows: P388D1 cell culture supernatant: 50% and spleen cell culture supernatant: 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Oncostatin M concentration was determined to be 106 pg/mL in neat P388D1 cell culture supernatant and 49 pg/mL in neat spleen cell culture supernatant.
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