AB263891

Mouse Oncostatin M ELISA Kit (OSM)

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Mouse Oncostatin M ELISA Kit (OSM) is a single-wash 90-min Simplestep used to quantify Mouse Oncostatin M (OSM) with a sensitivity of 4.36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Oncostatin-M, OSM, Osm

3 Images

Sandwich ELISA - Mouse Oncostatin M ELISA Kit (OSM) (AB263891)

sELISA

Supplier Data

Sandwich ELISA - Mouse Oncostatin M ELISA Kit (OSM) (AB263891)

Example of mouse Oncostatin M standard curve in Sample Diluent NS.

The Oncostatin M standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Oncostatin M ELISA Kit (OSM) (AB263891)

Interpolated concentrations of native Oncostatin M in mouse P388D1 cell culture supernatant and mouse spleen cell culture supernatant samples.

The concentrations of Oncostatin M were measured in duplicates, interpolated from the Oncostatin M standard curves and corrected for sample dilution. Undiluted samples are as follows : P388D1 cell culture supernatant : 50% and spleen cell culture supernatant : 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Oncostatin M concentration was determined to be 106 pg/mL in neat P388D1 cell culture supernatant and 49 pg/mL in neat spleen cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse Oncostatin M ELISA Kit (OSM) (AB263891)

Mouse Oncostatin M standard curve comparison.

Standard Curve comparison between Mouse Oncostatin M SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.36 pg/mL

Range

11.71875 - 750 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse Oncostatin M ELISA Kit (OSM) (ab263891) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Oncostatin M (OSM) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Oncostatin M (OSM) with 4.36 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Oncostatin M (OSM) is a growth regulator. Oncostatin M inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Oncostatin M uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). It is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. Rat Oncostatin M is 73% identical to mouse Oncostatin M.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 101 %", "average": "= 99" }, { "sample": "Serum", "range": "86 - 88 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "85 - 89 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "83 - 94 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "79 - 91 %", "average": "= 86" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab263891-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB263891-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB263891-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB263891-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Oncostatin M (OSM) Detector Antibody", "number":"AB263891-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB263891-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB263891-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB263891-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB263891-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Oncostatin M (OSM) Lyophilized Recombinant Protein", "number":"AB263891-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Oncostatin M (OSM) Capture Antibody", "number":"AB263891-CMP02", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oncostatin M (OSM) also known as Oncostatin M protein or OSM protein is a cytokine with significant roles in inflammation and hematopoiesis. This protein weighs around 28 kDa and is expressed mainly in activated T cells monocytes and macrophages. It interacts with specific receptors on the cell surface to transmit signals that regulate cell growth and function. Oncostatin M is part of the interleukin-6 (IL-6) family contributing to various cellular processes.

Biological function summary

Oncostatin M modulates a range of cellular activities including cell proliferation differentiation and apoptosis. This cytokine affects diverse cell types such as fibroblasts endothelial cells and epithelial cells showing particular influence in processes like tissue remodeling and immune response. It acts as a monomer and does not need assembly into a larger complex to exert its function. Its ability to trigger gene expression differentiates it from other closely related cytokines.

Pathways

Oncostatin M involves itself in significant pathways like the JAK/STAT signaling pathway and the MAPK pathway. Through these pathways it promotes signal transduction essential for immune regulation and cellular response to external stimuli. OSM interacts with proteins like the gp130 receptor a common signaling component for cytokine receptors in its family and influences downstream effectors involved in these pathways.

Oncostatin M associates itself with inflammatory diseases such as rheumatoid arthritis and cardiovascular disorders. Its elevated levels in pathological conditions highlight its role in exacerbating inflammatory responses. In rheumatoid arthritis OSM promotes synovial inflammation while in cardiovascular diseases it may influence vascular remodeling. Connections to inflammatory proteins such as IL-6 further highlight its part in driving disease pathogenesis making it an intriguing target for therapeutic intervention.

style

left-column

Product protocols

style

left-column

Target data

Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity). Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity).

See full target information Osm

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biology 14: PubMed40282226

2025

Mitigating Diabetic Cardiomyopathy: The Synergistic Potential of Sea Buckthorn and Metformin Explored via Bioinformatics and Chemoinformatics.

Applications

Unspecified application

Species

Unspecified reactive species

Kamran Safavi,Navid Abedpoor,Fatemeh Hajibabaie,Elina Kaviani

Breast cancer research : BCR 26:167 PubMed39593161

2024

Toll-Like receptor 3-mediated interferon-β production is suppressed by oncostatin m and a broader epithelial-mesenchymal transition program.

Applications

Unspecified application

Species

Unspecified reactive species

Noah M Chernosky,Ilaria Tamagno,Kelsey L Polak,E Ricky Chan,Xueer Yuan,Mark W Jackson

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Oncostatin M ELISA Kit (OSM)

Sandwich ELISA - Mouse Oncostatin M ELISA Kit (OSM) (AB263891)

Sandwich ELISA - Mouse Oncostatin M ELISA Kit (OSM) (AB263891)

Sandwich ELISA - Mouse Oncostatin M ELISA Kit (OSM) (AB263891)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise