Mouse Osteocalcin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Osteocalcin in Mouse in Plasma, Serum, Other biological fluids samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.39 - 25 ng/mL
- Sensitivity
- = 0.23 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The carboxylated form is one of the main organic components of the bone matrix, which constitutes 1-2% of the total bone protein: it acts as a negative regulator of bone formation and is required to limit bone formation without impairing bone resorption or mineralization (PubMed:8684484). The carboxylated form binds strongly to apatite and calcium (PubMed:17693256). The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development (PubMed:17693256, PubMed:20655470, PubMed:20655471, PubMed:21333348, PubMed:24074871). Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure (PubMed:17693256, PubMed:20655470, PubMed:20655471). Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner (PubMed:21333348). Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA) (PubMed:24074871, PubMed:28851741). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development (PubMed:24074871).
Alternative names
Ocn, Bglap, Osteocalcin, OG1, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, BGP
What's included?
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Mouse Osteocalcin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Osteocalcin in Mouse in Plasma, Serum, Other biological fluids samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.39 - 25 ng/mL
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 0.23 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 99 | Range 92 - 109 % |
Sample type Cell culture media | Average % = 90 | Range 85 - 97 % |
Sample type EDTA Plasma | Average % = 99 | Range 93 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
This Osteocalcin (Mouse) ELISA kit (ab285236, E4763) is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to mouse Osteocalcin alpha.
Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for mouse Osteocalcin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of Osteocalcin bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow.
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of mouse Osteocalcin. The concentration of mouse Osteocalcin in the samples can be calculated by comparing the OD of the samples to the standard curve.
This product is manufactured by BioVision, an Abcam company and was previously called E4763 Osteocalcin (Mouse) ELISA Kit. E4763-100 is the same size as the 96 test size of ab285236.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Osteocalcin also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP) is a small non-collagenous protein with a molecular weight of approximately 5.8 kDa. This protein is mainly expressed in osteoblasts which are bone-forming cells. It is an important component of the bone matrix and is involved in bone mineralization. Osteocalcin binds to calcium and incorporates into the hydroxyapatite crystals in the bone helping stabilize the bone structure. Researchers often study this protein as a marker for bone formation and turnover.
- Biological function summary
Osteocalcin plays a significant role in regulating glucose metabolism and fat deposition. It acts as a hormone from the bone and influences energy metabolism and insulin sensitivity. Osteocalcin undergoes carboxylation a post-translational modification necessary for its function in bone. Beyond its structural role demineralized osteocalcin has been found to impact pancreatic β-cell function adipose tissue and male fertility although the pathways in these processes are not entirely understood yet.
- Pathways
Osteocalcin is associated with the energy metabolism and bone remodeling pathways. It interacts with the insulin signaling pathway influencing insulin secretion by pancreatic β-cells and boosting insulin sensitivity. This interaction involves proteins like insulin receptor substrate 1 (IRS1) and glucose transporter type 4 (GLUT4) which play roles in glucose uptake. Osteocalcin also connects to bone remodeling pathways by influencing osteoclast differentiation and activity through osteoprotegrin and receptor activator of nuclear factor kappa-B ligand (RANKL).
- Associated diseases and disorders
Dysregulation of osteocalcin levels associates with metabolic syndrome and osteoporosis. It is linked with insulin resistance affecting glucose homeostasis and potentially contributing to diabetes development. Osteocalcin's involvement in bone density maintenance also makes it relevant in osteoporosis where bone formation balance is disrupted. The receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegrin which osteocalcin interacts with are other proteins connected to the pathogenesis of these conditions. Understanding osteocalcin's function helps in exploring therapeutic targets for these diseases.
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ELISA - Mouse Osteocalcin ELISA Kit (ab285236)
Osteocalcin (Mouse) ELISA Kit Standard Curve.
Typical standard curve and data is provided below for reference only. A standard curve must be run with each assay
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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