AB285236

Mouse Osteocalcin ELISA Kit

Name: Mouse Osteocalcin ELISA Kit (ab285236) | Abcam
Brand: Abcam Limited
SKU: AB285236

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Mouse Osteocalcin ELISA Kit is a sandwich ELISA designed to quantify Mouse Osteocalcin with a sensitivity of 0.23 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.39 - 25 ng/mL

View Alternative Names

Ocn, Bglap, Osteocalcin, OG1, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, BGP

1 Images

ELISA - Mouse Osteocalcin ELISA Kit (AB285236)

ELISA

Supplier Data

ELISA - Mouse Osteocalcin ELISA Kit (AB285236)

Osteocalcin (Mouse) ELISA Kit Standard Curve.

Typical standard curve and data is provided below for reference only. A standard curve must be run with each assay

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.23 ng/mL

Range

0.39 - 25 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Osteocalcin ELISA Kit ab285236 is a sandwich ELISA to measure Mouse Osteocalcin in serum, plasma, other biological fluids with a sensitivity of 0.23 ng/ml.

How the assay works

Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for mouse Osteocalcin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of Osteocalcin bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of mouse Osteocalcin. The concentration of mouse Osteocalcin in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Osteocalcin ELISA Kit ab285236 protocol summary

1. Add standards and samples to appropriate wells.Incubate
2. Add Biotinylated Detection Antibody. Incubate
3. Wash
4. Add HRP Conjugate. Incubate
5. Wash
6. Add Substrate Reagent. Incubate
7. Add stop solution and read at 450 nm

This product is manufactured by BioVision, an Abcam company and was previously called E4763 Osteocalcin (Mouse) ELISA Kit. E4763-100 is the same size as the 96 test size of ab285236.

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Recovery

[ { "sample": "Serum", "range": "92 - 109 %", "average": "= 99" }, { "sample": "Cell culture media", "range": "85 - 97 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "93 - 109 %", "average": "= 99" } ]

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285236-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285236-CMP08", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X HRP-Conjugate", "number":"AB285236-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Reference Standard", "number":"AB285236-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Biotinylated Detection Antibody", "number":"AB285236-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285236-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"Substrate", "number":"AB285236-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"Reference Standard and Sample Diluent", "number":"AB285236-CMP07", "productcode":"" }, { "size":"1 x 14 mL", "name":"Biotinylated Detection Antibody Diluent", "number":"AB285236-CMP05", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Sealer", "number":"AB285236-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285236-CMP10", "productcode":"" }, { "size":"1 x 14 mL", "name":"HRP-Conjugate Diluent", "number":"AB285236-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteocalcin also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP) is a small non-collagenous protein with a molecular weight of approximately 5.8 kDa. This protein is mainly expressed in osteoblasts which are bone-forming cells. It is an important component of the bone matrix and is involved in bone mineralization. Osteocalcin binds to calcium and incorporates into the hydroxyapatite crystals in the bone helping stabilize the bone structure. Researchers often study this protein as a marker for bone formation and turnover.

Biological function summary

Osteocalcin plays a significant role in regulating glucose metabolism and fat deposition. It acts as a hormone from the bone and influences energy metabolism and insulin sensitivity. Osteocalcin undergoes carboxylation a post-translational modification necessary for its function in bone. Beyond its structural role demineralized osteocalcin has been found to impact pancreatic β-cell function adipose tissue and male fertility although the pathways in these processes are not entirely understood yet.

Pathways

Osteocalcin is associated with the energy metabolism and bone remodeling pathways. It interacts with the insulin signaling pathway influencing insulin secretion by pancreatic β-cells and boosting insulin sensitivity. This interaction involves proteins like insulin receptor substrate 1 (IRS1) and glucose transporter type 4 (GLUT4) which play roles in glucose uptake. Osteocalcin also connects to bone remodeling pathways by influencing osteoclast differentiation and activity through osteoprotegrin and receptor activator of nuclear factor kappa-B ligand (RANKL).

Dysregulation of osteocalcin levels associates with metabolic syndrome and osteoporosis. It is linked with insulin resistance affecting glucose homeostasis and potentially contributing to diabetes development. Osteocalcin's involvement in bone density maintenance also makes it relevant in osteoporosis where bone formation balance is disrupted. The receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegrin which osteocalcin interacts with are other proteins connected to the pathogenesis of these conditions. Understanding osteocalcin's function helps in exploring therapeutic targets for these diseases.

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Product protocols

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Target data

The carboxylated form is one of the main organic components of the bone matrix, which constitutes 1-2% of the total bone protein : it acts as a negative regulator of bone formation and is required to limit bone formation without impairing bone resorption or mineralization (PubMed : 8684484). The carboxylated form binds strongly to apatite and calcium (PubMed : 17693256).. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development (PubMed : 17693256, PubMed : 20655470, PubMed : 20655471, PubMed : 21333348, PubMed : 24074871). Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure (PubMed : 17693256, PubMed : 20655470, PubMed : 20655471). Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes : acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner (PubMed : 21333348). Also acts as a regulator of brain development : osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA) (PubMed : 24074871, PubMed : 28851741). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development (PubMed : 24074871).

See full target information Bglap

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse Osteocalcin ELISA Kit

ELISA - Mouse Osteocalcin ELISA Kit (AB285236)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise