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AB100734

Mouse Osteopontin ELISA Kit

5

(2 Reviews)

|

(10 Publications)

Mouse Osteopontin ELISA Kit is a sandwich ELISA designed to quantify Mouse Osteopontin with a sensitivity of 4 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 2.74 - 2000 pg/mL

View Alternative Names

Eta-1, Op, Spp-1, Spp1, Osteopontin, 2AR, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Secreted phosphoprotein 1, SPP-1

1 Images
Sandwich ELISA - Mouse Osteopontin ELISA Kit (AB100734)
  • sELISA

Unknown

Sandwich ELISA - Mouse Osteopontin ELISA Kit (AB100734)

Representative standard curve using ab100734

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 4 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Complementary Products

Primary Antibodies

AB63856

Anti-Osteopontin antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody (AB63856)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB285236

Mouse Osteocalcin ELISA Kit

ELISA - Mouse Osteocalcin ELISA Kit (AB285236)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Osteopontin ELISA Kit ab100734 is a sandwich ELISA to measure Mouse Osteopontin in serum, plasma, cell culture supernatant with a sensitivity of 4 pg/ml.

How the assay works

This assay employs an antibody specific for Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Osteopontin ELISA Kit ab100734 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
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Recovery

[ { "sample": "Cell culture supernatant", "range": "78 - 105 %", "average": "= 85.46" }, { "sample": "Serum", "range": "103 - 122 %", "average": "= 115.6" }, { "sample": "Plasma", "range": "87 - 130 %", "average": "= 110.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100734-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100734-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100734-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse Osteopontin", "number":"AB100734-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Osteopontin Microplate (12 x 8 wells)", "number":"AB100734-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100734-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100734-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100734-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse Osteopontin Standard (lyophilized)", "number":"AB100734-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100734-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.
Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.
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Product protocols

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Target data

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
See full target information Spp1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Bone research 13:43 PubMed40169533

2025

KIAA1199 (CEMIP) regulates adipogenesis and whole-body energy metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Kaikai Shi,Nicholas Ditzel,Weimin Qiu,Michaela Tencerova,Louise Himmelstrup Dreyer Nielsen,Florence Figeac,Alexander Rauch,Yuhang Liu,Jiuyuan Tao,Veronika Sramkova,Lenka Rossmeislova,Greet Kerckhofs,Tatjana N Parac-Vogt,Sébastien de Bournonville,Thomas Levin Andersen,Mikael Rydén,Moustapha Kassem

iScience 27:108725 PubMed38226160

2024

SORCS2 activity in pancreatic α-cells safeguards insulin granule formation and release from glucose-stressed β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Oleksandra Kalnytska,Per Qvist,Séverine Kunz,Thomas Conrad,Thomas E Willnow,Vanessa Schmidt

Frontiers in endocrinology 14:1252727 PubMed37810891

2023

Potential involvement of the bone marrow in experimental Graves' disease and thyroid eye disease.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Gulbins,Mareike Horstmann,Simone Keitsch,Matthias Soddemann,Barbara Wilker,Gregory C Wilson,Ryan Zeidan,Gary D Hammer,Anke Daser,Nikolaos E Bechrakis,Gina-Eva Görtz,Anja Eckstein

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2300897 PubMed37218542

2023

Blockage of Osteopontin-Integrin β3 Signaling in Infrapatellar Fat Pad Attenuates Osteoarthritis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyang Dai,Yuwei Zhu,Xu Li,Zuru Liang,Shunxiang Xu,Shian Zhang,Zhe Zhang,Shanshan Bai,Wenxue Tong,Mingde Cao,Ye Li,Xiaobo Zhu,Wei Liu,Yuantao Zhang,Liang Chang,Patrick Shu-Hang Yung,Kevin Ki-Wai Ho,Jiankun Xu,To Ngai,Ling Qin

Nature communications 14:2016 PubMed37037828

2023

KIAA1199 deficiency enhances skeletal stem cell differentiation to osteoblasts and promotes bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Kaikai Shi,Nicholas Ditzel,Weimin Qiu,Florence Figeac,Louise Himmelstrup Dreyer Nielsen,Michaela Tencerova,Justyna Magdalena Kowal,Ming Ding,Christina Møller Andreasen,Thomas Levin Andersen,Moustapha Kassem

Nature communications 13:427 PubMed35058428

2022

Macrophages in epididymal adipose tissue secrete osteopontin to regulate bone homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyang Dai,Jiankun Xu,Xu Li,Le Huang,Chelsea Hopkins,Honglian Wang,Hao Yao,Jie Mi,Lizhen Zheng,Jiali Wang,Wenxue Tong,Dick Ho-Kiu Chow,Ye Li,Xuan He,Peijie Hu,Ziyi Chen,Haiyue Zu,Yixuan Li,Yao Yao,Qing Jiang,Ling Qin

Journal of lipid research 63:100153 PubMed34808192

2021

Oxidized phospholipids cause changes in jejunum mucus that induce dysbiosis and systemic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Pallavi Mukherjee,Arnab Chattopadhyay,Victor Grijalva,Nasrin Dorreh,Venu Lagishetty,Jonathan P Jacobs,Bethan L Clifford,Thomas Vallim,Julia J Mack,Mohamad Navab,Srinivasa T Reddy,Alan M Fogelman

The American journal of pathology 186:3054-3063 PubMed27648615

2016

Adipocyte-Lineage Cells Support Growth and Dissemination of Multiple Myeloma in Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy N Trotter,Justin T Gibson,Tshering Lama Sherpa,Pramod S Gowda,Deniz Peker,Yang Yang

PloS one 9:e88764 PubMed24558422

2014

Respiratory syncytial virus (RSV) infection in elderly mice results in altered antiviral gene expression and enhanced pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Terianne M Wong,Sandhya Boyapalle,Viviana Sampayo,Huy D Nguyen,Raminder Bedi,Siddharth G Kamath,Martin L Moore,Subhra Mohapatra,Shyam S Mohapatra

PloS one 7:e31174 PubMed22347449

2012

Ameliorated ConA-induced hepatitis in the absence of PKC-theta.

Applications

Unspecified application

Species

Mouse

Xianfeng Fang,Ruiqing Wang,Jian Ma,Yan Ding,Weirong Shang,Zuoming Sun
View all publications
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Product promise

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