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AB315312

Mouse Osteopontin ELISA Kit

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Mouse Osteopontin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Osteopontin with a sensitivity of 6.869 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Eta-1, Op, Spp-1, Spp1, Osteopontin, 2AR, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Secreted phosphoprotein 1, SPP-1

4 Images
Sandwich ELISA - Mouse Osteopontin ELISA Kit (AB315312)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Osteopontin ELISA Kit (AB315312)

Example of mouse Osteopontin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Osteopontin ELISA Kit (AB315312)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Osteopontin ELISA Kit (AB315312)

Interpolated concentration of native Osteopontin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Unstimulated splenocyte cell culture supernatant 50% and stimulated splenocyte cell culture supernatant 5%.  Stimulated splenocytes were treated with 1.5% PHA-M, 10 ng/mL PMA, and 5 µg/mL LPS for 72 hours.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse Osteopontin ELISA Kit (AB315312)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Osteopontin ELISA Kit (AB315312)

Standard curve comparison between Mouse Osteopontin SimpleStep ELISA® kit (ab315312) and traditional ELISA kit from leading competitor. The SimpleStep ELISA® kit shows increased sensitivity.

Sandwich ELISA - Mouse Osteopontin ELISA Kit (AB315312)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Osteopontin ELISA Kit (AB315312)

Interpolated concentration of native Osteopontin was measured in duplicate at different sample concentrations. Undiluted samples are 1% serum, plasma (EDTA), plasma (citrate), and plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 6.869 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Osteopontin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteopontin protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, and Cell culture supernatant. Quantitate Mouse Osteopontin with 6.869 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.6" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 103 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "97 - 97 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "90 - 107 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "94 - 99 %", "average": "= 96" }, { "sample": "Cell culture supernatant", "range": "96 - 105 %", "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.
Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.
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Product protocols

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Target data

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
See full target information Spp1
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websiteProtocolBooklet
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