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AB100733

Mouse Osteoprotegerin ELISA Kit

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(8 Publications)

Mouse Osteoprotegerin ELISA Kit is a Sandwich ELISA for the measurement of Mouse Osteoprotegerin in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Ocif, Opg, Tnfrsf11b, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin

2 Images
Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (AB100733)
  • sELISA

Unknown

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (AB100733)

Representative standard curve using ab100733

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (AB100733)
  • sELISA

Unknown

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (AB100733)

Representative standard curve using ab100733

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 1 pg/mL

Range

1.65 - 1200 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Osteoprotegerin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Osteoprotegerin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse Osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteoprotegerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "81 - 102 %", "average": "= 88.7" }, { "sample": "Serum", "range": "82 - 104 %", "average": "= 91.7" }, { "sample": "Plasma", "range": "65 - 89 %", "average": "= 81.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100733-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100733-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100733-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse Osteoprotegerin", "number":"AB100733-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100733-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse Osteoprotegerin Standard (lyophilized)", "number":"AB100733-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100733-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100733-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100733-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Osteoprotegerin Microplate (12 x 8 wells)", "number":"AB100733-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteoprotegerin (OPG) also known as osteoclastogenesis inhibitory factor is a glycoprotein with a mass of approximately 60 kDa. It acts as a soluble decoy receptor for receptor activator of nuclear factor kappa-Β ligand (RANKL) and is encoded by the TNFRSF11B gene. OPG is mainly expressed in osteoblasts fibroblasts and endothelial cells. Its expression can be found in diverse tissues throughout the body including the liver and lungs.
Biological function summary

OPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.

Pathways

OPG operates within the RANK/RANKL/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.

OPG has a significant association with conditions such as osteoporosis and vascular calcification. In osteoporosis an imbalance in the RANKL/OPG ratio can lead to excessive bone loss due to increased osteoclast activity. In vascular calcification OPG appears to play a protective role likely linked with regulatory mechanisms involving RANKL and TRAIL. Understanding these interactions opens potential therapeutic avenues particularly in managing bone-related and cardiovascular conditions.
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Product protocols

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Target data

Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
See full target information Tnfrsf11b
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 28:74 PubMed34749728

2021

Restoration of 5-methoxytryptophan protects against atherosclerotic chondrogenesis and calcification in ApoE mice fed high fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Guan-Lin Lee,Tsai-Lien Liao,Jing-Yiing Wu,Kenneth K Wu,Cheng-Chin Kuo

Biomaterials 276:121025 PubMed34298444

2021

Dual actions of osteoclastic-inhibition and osteogenic-stimulation through strontium-releasing bioactive nanoscale cement imply biomaterial-enabled osteoporosis therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Na-Hyun Lee,Min Sil Kang,Tae-Hyun Kim,Dong Suk Yoon,Nandin Mandakhbayar,Seung Bin Jo,Hye Sung Kim,Jonathan C Knowles,Jung-Hwan Lee,Hae-Won Kim

Journal of translational autoimmunity 3:100028 PubMed32743513

2020

Programmed death ligand 2 - A link between inflammation and bone loss in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Stinne R Greisen,Tue W Kragstrup,Jesper Skovhus Thomsen,Aida Solhøj Hansen,Akilan Krishnamurthy,Kim Hørslev-Petersen,Merete Lund Hetland,Kristian Stengaard-Pedersen,Mikkel Østergaard,Lykke Midtbøll Ørnbjerg,Peter Junker,Arlene H Sharpe,Gordon J Freeman,Lakshmanan Annamalai,Malene Hvid,Søren K Moestrup,Ellen-Margrethe Hauge,Anca Irinel Catrina,Bent Deleuran

Life sciences 255:117827 PubMed32450170

2020

Selective serotonin reuptake inhibitors (SSRI) affect murine bone lineage cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Durham,Yuhua Zhang,Amanda LaRue,Amy Bradshaw,James Cray

Arteriosclerosis, thrombosis, and vascular biology 39:432-445 PubMed30626205

2019

TLR2 Promotes Vascular Smooth Muscle Cell Chondrogenic Differentiation and Consequent Calcification via the Concerted Actions of Osteoprotegerin Suppression and IL-6-Mediated RANKL Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Guan-Lin Lee,Chang-Ching Yeh,Jing-Yiing Wu,Hui-Chen Lin,Yi-Fu Wang,Ya-Yi Kuo,Yi-Ting Hsieh,Yu-Juei Hsu,Cheng-Chin Kuo

Bone 114:90-108 PubMed29908298

2018

Hydrogen sulfide epigenetically mitigates bone loss through OPG/RANKL regulation during hyperhomocysteinemia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jyotirmaya Behera,Akash K George,Michael J Voor,Suresh C Tyagi,Neetu Tyagi

Scientific reports 7:13241 PubMed29038439

2017

Analysis of Nkx3.1:Cre-driven Erk5 deletion reveals a profound spinal deformity which is linked to increased osteoclast activity.

Applications

Unspecified application

Species

Unspecified reactive species

Carolyn J Loveridge,Rob J van 't Hof,Gemma Charlesworth,Ayala King,Ee Hong Tan,Lorraine Rose,Anna Daroszewska,Amanda Prior,Imran Ahmad,Michelle Welsh,Ernest J Mui,Catriona Ford,Mark Salji,Owen Sansom,Karen Blyth,Hing Y Leung

Arteriosclerosis, thrombosis, and vascular biology 35:558-69 PubMed25593133

2015

Maternal high-fat diet exaggerates atherosclerosis in adult offspring by augmenting periaortic adipose tissue-specific proinflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Noriyuki Wakana,Daisuke Irie,Masakazu Kikai,Kensuke Terada,Keita Yamamoto,Hiroyuki Kawahito,Taku Kato,Takehiro Ogata,Tomomi Ueyama,Satoaki Matoba,Hiroyuki Yamada
View all publications
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