AB203365

Mouse Osteoprotegerin ELISA Kit (TNFRSF11B)

Recombinant
SimpleStep
What is this?

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(3 Publications)

Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) is a single-wash 90-min Simplestep used to quantify Mouse Osteoprotegerin (TNFRSF11B) with a sensitivity of 5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ocif, Opg, Tnfrsf11b, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin

4 Images

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)

sELISA

Supplier Data

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)

Interpolated concentrations of Osteoprotegerin in mouse lung day 5 supernatant, serum and plasma (heparin) samples.

The concentrations of Osteoprotegerin were measured in duplicates, interpolated from the Osteoprotegerin standard curve and corrected for sample dilution. Note that 1X Diluted samples were neat. The interpolated, dilution factor-corrected values are plotted in pg of Osteoprotegerin per mL of neat sample (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)

Example of Osteoprotegerin standard curve for prepared in Sample Diluent 50BS measurements.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)

Mouse Osteoprotegerin standard curve comparison data.

Standard curve comparison between mouse Osteoprotegerin SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.

sELISA

Supplier Data

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)

Example of Osteoprotegerin standard curve for prepared in Sample Diluent NS measurements.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (ab203365) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteoprotegerin (TNFRSF11B) protein in cell culture supernatant, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Osteoprotegerin (TNFRSF11B) with 5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Osteoprotegerin acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Osteoprotegerin inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. Osteoprotegerin may also play a role in preventing arterial calcification. Osteoprotegerin may act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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Recovery

[ { "sample": "Serum", "range": "90 - 101.3 %", "average": "= 96.1" }, { "sample": "Heparin Plasma", "range": "117.9 - 132.8 %", "average": "= 123.4" }, { "sample": "Cell culture media", "range": "102.1 - 106.3 %", "average": "= 104.9" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab203365-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB203365-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB203365-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB203365-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB203365-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB203365-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB203365-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB203365-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Osteoprotegerin Lyophilized Recombinant Protein", "number":"AB203365-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB203365-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Osteoprotegerin Capture Antibody", "number":"AB203365-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Osteoprotegerin Detector Antibody", "number":"AB203365-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteoprotegerin (OPG) also known as osteoclastogenesis inhibitory factor is a glycoprotein with a mass of approximately 60 kDa. It acts as a soluble decoy receptor for receptor activator of nuclear factor kappa-Β ligand (RANKL) and is encoded by the TNFRSF11B gene. OPG is mainly expressed in osteoblasts fibroblasts and endothelial cells. Its expression can be found in diverse tissues throughout the body including the liver and lungs.

Biological function summary

OPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.

Pathways

OPG operates within the RANK/RANKL/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.

OPG has a significant association with conditions such as osteoporosis and vascular calcification. In osteoporosis an imbalance in the RANKL/OPG ratio can lead to excessive bone loss due to increased osteoclast activity. In vascular calcification OPG appears to play a protective role likely linked with regulatory mechanisms involving RANKL and TRAIL. Understanding these interactions opens potential therapeutic avenues particularly in managing bone-related and cardiovascular conditions.

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Product protocols

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Target data

Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.

See full target information Tnfrsf11b

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:4820 PubMed35973996

2022

A TrkB agonist prodrug prevents bone loss via inhibiting asparagine endopeptidase and increasing osteoprotegerin.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xiong,Jianming Liao,Xia Liu,Zhaohui Zhang,Jonathan Adams,Roberto Pacifici,Keqiang Ye

Nature communications 13:771 PubMed35140213

2022

An optogenetic approach for regulating human parathyroid hormone secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhui Liu,Lu Zhang,Nan Hu,Jie Shao,Dazhi Yang,Changshun Ruan,Shishu Huang,Liping Wang,William W Lu,Xinzhou Zhang,Fan Yang

Nature communications 11:431 PubMed31969567

2020

Environmental arginine controls multinuclear giant cell metabolism and formation.

Applications

Unspecified application

Species

Unspecified reactive species

Julia S Brunner,Loan Vulliard,Melanie Hofmann,Markus Kieler,Alexander Lercher,Andrea Vogel,Marion Russier,Johanna B Brüggenthies,Martina Kerndl,Victoria Saferding,Birgit Niederreiter,Alexandra Junza,Annika Frauenstein,Carina Scholtysek,Yohei Mikami,Kristaps Klavins,Gerhard Krönke,Andreas Bergthaler,John J O'Shea,Thomas Weichhart,Felix Meissner,Josef S Smolen,Paul Cheng,Oscar Yanes,Jörg Menche,Peter J Murray,Omar Sharif,Stephan Blüml,Gernot Schabbauer

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Osteoprotegerin ELISA Kit (TNFRSF11B)

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)

Sandwich ELISA - Mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (AB203365)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise