Mouse OX40 ELISA Kit (TNFRSF4)
- Recombinant
- SimpleStep
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Mouse OX40 ELISA Kit (TNFRSF4) is a single-wash 90-min Simplestep used to quantify Mouse OX40 (TNFRSF4) with a sensitivity of 33 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CD134, Ox40, Txgp1, Tnfrsf4, Tumor necrosis factor receptor superfamily member 4, OX40 antigen, OX40L receptor
- sELISA
Unknown
Sandwich ELISA - Mouse OX40 ELISA Kit (TNFRSF4) (AB253218)
Interpolated concentrations of native OX40 in mouse serum and plasma samples.
The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 546.8 pg/mL in serum, 530.6 pg/mL in plasma (citrate), 522.3 pg/mL in plasma (EDTA), and 626.3 pg/mL in plasma (heparin).
- sELISA
Unknown
Sandwich ELISA - Mouse OX40 ELISA Kit (TNFRSF4) (AB253218)
Interpolated concentrations of native OX40 in mouse spleen supernatant sample treated with 2.5 µg/mL Concanavalin A for 72 hours.
The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows : spleen supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 564.3 pg/mL in spleen supernatant.
- sELISA
Unknown
Sandwich ELISA - Mouse OX40 ELISA Kit (TNFRSF4) (AB253218)
Example of mouse OX40 standard curve in 1X Cell Extraction Buffer PTR.
The OX40 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Mouse OX40 ELISA Kit (TNFRSF4) (AB253218)
Example of mouse OX40 standard curve in Sample Diluent NS.
The OX40 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Mouse OX40 ELISA Kit (TNFRSF4) (AB253218)
Interpolated concentrations of native OX40 in treated and untreated mouse splenocyte cell extract samples based on a 500 μg/mL extract load.
The concentrations of OX40 were measured in duplicate and interpolated from the OX40 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 2,184.1 pg /mL in treated splenocyte cell extract samples and 605 pg/mL in untreated splenocyte cell extract samples.
Reactivity data
Product details
Mouse OX40 ELISA Kit (TNFRSF4) (ab253218) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OX40 protein in cell culture supernatant, cell lysate, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse OX40 with 33 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
OX40, also known as TNFRSF4 and/or CD134, is a constitutively expressed transmembrane receptor found on T-cells. OX40 serves as a secondary checkpoint for immunological responses through the binding of TNF receptor associated factor (TRAF) proteins. OX40 and its predominant ligand, OX40L, regulate adaptive T cell immune responses. Clinically, OX40 is the target of several immunotherapy antibody treatments to promote antitumor immunity.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
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Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.
Pathways
CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.
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