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AB200014

Mouse P-Selectin (CD62P) ELISA Kit

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(4 Publications)

Mouse P-Selectin ELISA Kit (CD62P) is a single-wash 90-min Simplestep used to quantify Mouse P-Selectin (CD62P) with a sensitivity of 4.16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD62P, Grmp, Selp, P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane protein, GMP-140, LECAM3, PADGEM

5 Images
Sandwich ELISA - Mouse P-Selectin (CD62P) ELISA Kit (AB200014)
  • sELISA

Lab

Sandwich ELISA - Mouse P-Selectin (CD62P) ELISA Kit (AB200014)

Mouse P-Selectin (CD62P) standard curve comparison data.

Standard curve comparison between mouse P-Selectin (CD62P) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.

Sandwich ELISA - Mouse P-Selectin (CD62P) ELISA Kit (AB200014)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse P-Selectin (CD62P) ELISA Kit (AB200014)

Example of P-Selectin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse P-Selectin (CD62P) ELISA Kit (AB200014)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse P-Selectin (CD62P) ELISA Kit (AB200014)

Example of P-Selectin standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse P-Selectin (CD62P) ELISA Kit (AB200014)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse P-Selectin (CD62P) ELISA Kit (AB200014)

Titration of lung and spleen extracts in 1X Cell Extraction Buffer PTR within the working range of the assay.

The 1X dilution for the lung extracts was 500 μg/mL, and for the spleen extracts 125 μg/mL. The interpolated P-Selectin concentrations multiplied by the total dilution factor are shown for each supernatant (mean +/- SD, n = 2).

ELISA - Mouse P-Selectin (CD62P) ELISA Kit (AB200014)
  • ELISA

Lab

ELISA - Mouse P-Selectin (CD62P) ELISA Kit (AB200014)

Titration of mouse serum, EDTA platelet poor plasma, citrate plasma, and heparin plasma within the working range of the assay.

The 1X dilution for serum and each plasma was at 2% matrix. The interpolated P-selectin concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 4.16 pg/mL

Range

23.4 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse P-Selectin ELISA Kit (CD62P) (ab200014) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of P-Selectin (CD62P) protein in cit plasma, serum, cell culture supernatant, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse P-Selectin (CD62P) with 4.16 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

P-Selectin is the largest member of the selectin family, which are adhesion molecules that ensure efficient recruitment of leukocytes to inflammation sites. It is stored in platelets and endothelial cells in Weibel-Palade bodies and α-granules, and is expressed by membrane translocation. P-Selectin's main function is to mediate the interaction of activated endothelial cells or platelets with leukocytes, and most importantly, leukocyte rolling after trauma. High soluble levels of P-Selectin are thought to be predictive of future cardiovascular events.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.21" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Serum", "range": "107 - 116 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "103 - 120 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "118 - 120 %", "average": "= 119" }, { "sample": "EDTA Plasma", "range": "119 - 126 %", "average": "= 122" }, { "sample": "Heparin Plasma", "range": "119 - 139 %", "average": "= 127" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab200014-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB200014-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB200014-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB200014-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse P-Selectin Lyophilized Recombinant Protein", "number":"AB200014-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB200014-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse P-Selectin Capture Antibody", "number":"AB200014-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB200014-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse P-Selectin Detector Antibody", "number":"AB200014-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB200014-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB200014-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB200014-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB200014-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62P also known as P-selectin is a cell adhesion molecule expressed on the surface of activated endothelial cells and platelets. It is a type of protein with a known molecular weight of approximately 140 kDa. P-selectin plays a mechanical role in mediating leukocyte rolling on the endothelium which is an initial step in the inflammatory response. This protein is rapidly translocated to the plasma membrane upon platelet activation and is stored in alpha granules of platelets and Weibel-Palade bodies of endothelial cells.
Biological function summary

P-selectin is critical in the recruitment of leukocytes to sites of tissue injury and infection. It facilitates the adhesion of leukocytes to the vascular endothelium by binding to its ligand PSGL-1 on the leukocyte surface. This interaction supports the transition of leukocytes from the circulation to the site of inflammation. P-selectin is also involved in thrombus formation as it contributes to the interactions between platelets leukocytes and endothelium in the vascular wall. It acts as part of a dynamic complex in the regulation of the innate immune response and hemostasis.

Pathways

CD62P plays an important role in both the inflammatory and coagulation pathways. It is an important mediator in the leukocyte adhesion cascade influencing leukocyte tethering and rolling on activated endothelium. The interaction of P-selectin with PSGL-1 initiates the signaling pathways that lead to firm adhesion and extravasation of leukocytes. In the coagulation pathway P-selectin enhances interactions between platelets and leukocytes which support thrombus formation. Its regulation and activity are tightly linked with other proteins such as E-selectin and L-selectin in coordinating vascular and systemic inflammatory responses.

Altered expression of CD62P is associated with various inflammatory conditions and cardiovascular diseases. In thrombosis increased levels of P-selectin enhance platelet aggregation and stability of the thrombus contributing to pathological clot formation. Similarly in atherosclerosis P-selectin facilitates the recruitment of monocytes to the vessel wall promoting plaque development. The protein's function relates to interactions with key inflammatory mediators and its dysregulation is often observed in conditions like chronic inflammatory diseases and acute myocardial infarction.
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Product protocols

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Target data

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B : ITGB3) and integrin alpha-V/beta-3 (ITGAV : ITGB3) (By similarity).
See full target information Selp
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 45:181-200 PubMed39651397

2024

Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Tang,Fuxiang Wei,Weihua Qiao,Xing Chen,Chenyang Ji,Wanzhi Yang,Xinyu Zhang,Sihan Chen,Yanyan Wu,Mingxing Jiang,Chenyu Ma,Weiqiang Shen,Qi Dong,Hong Cao,Minghui Xie,Ziwen Cai,Li Xu,Jiawei Shi,Nianguo Dong,Junwei Chen,Ning Wang

PLoS pathogens 20:e1011777 PubMed38913740

2024

Characterization of Unique Pathological Features of COVID-Associated Coagulopathy: Studies with AC70 hACE2 Transgenic Mice Highly Permissive to SARS-CoV-2 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandra K Drelich,Kempaiah Rayavara,Jason Hsu,Panatda Saenkham-Huntsinger,Barbara M Judy,Vivian Tat,Thomas G Ksiazek,Bi-Hung Peng,Chien-Te K Tseng

Aging cell 23:e14050 PubMed38098255

2023

Viral uptake and pathophysiology of the lung endothelial cells in age-associated severe SARS-CoV-2 infection models.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Tsumita,Ryo Takeda,Nako Maishi,Yasuhiro Hida,Michihito Sasaki,Yasuko Orba,Akihiko Sato,Shinsuke Toba,Wataru Ito,Takahito Teshirogi,Yuya Sakurai,Tomohiro Iba,Hisamichi Naito,Hitoshi Ando,Haruhisa Watanabe,Amane Mizuno,Toshiki Nakanishi,Aya Matsuda,Ren Zixiao,Ji-Won Lee,Tadahiro Iimura,Hirofumi Sawa,Kyoko Hida

Evidence-based complementary and alternative medicine : eCAM 2022:6606423 PubMed36588591

2022

Humic Acids Inhibit Platelet Activation to Reduce Venous Thromboembolism in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Tao Lan,Ya-Ting Zheng,Zhou-Jie Tong,Cong Zhang,Xiao-Yan Cong,Zhi-Hao Wang
View all publications
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