Mouse p21 ELISA Kit (CDKN1A) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse p21 (CDKN1A) in Cell culture extracts, Tissue Extracts samples.
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- Physiological reports 6:2018Adaptive immune responses are altered in adult mice following neonatal hyperoxia.Applications:Unspecified applicationReactive species:MouseVasantha H S Kumar,Huamei Wang,Lori NielsenPubMed 29368801
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
62.5 - 4000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 2.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (PubMed:25329316). Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (By similarity). Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (By similarity).
Alternative names
Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein, p21, Cdkn1a, Cip1, Waf1
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Recommended products
Mouse p21 ELISA Kit (CDKN1A) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse p21 (CDKN1A) in Cell culture extracts, Tissue Extracts samples.
Alternative names
Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein, p21, Cdkn1a, Cip1, Waf1
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
62.5 - 4000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 2.7 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cell Extract | n 5 | mean - | SD - | C.V. 3.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cell Extract | n 3 | mean - | SD - | C.V. 7.8 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse p21 ELISA Kit (CDKN1A) (ab205576) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p21 (CDKN1A) protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse p21 (CDKN1A) with 2.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
p21 binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. p21 functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, p21 inhibits the kinase activity of the cyclin D-CDK4 complex. p21 may be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The p21 protein also known as CDKN1A is an important regulator of cell cycle progression. It acts as a cyclin-dependent kinase inhibitor where it binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes. The molecular weight of p21 is approximately 21 kDa. Cellular expression of p21 is diverse; it occurs in various tissues reflecting its broad role in maintaining cell cycle control. This protein's expression level is often assessed using techniques such as p21 western blotting p21 ELISA and p21 IHC staining.
- Biological function summary
P21 plays a critical role in cell cycle regulation and DNA damage response. It associates with the p53 tumor suppressor protein forming an essential part of the p53 signaling pathway under stress conditions. p21 arrests cells in G1 phase allowing DNA repair or leading to cellular senescence. Through these mechanisms p21 integrates signals from stress and damage emphasizing its importance as a mediator in cellular checkpoint control.
- Pathways
P21 is closely involved in the p53 and the Transforming Growth Factor-beta (TGF-β) signaling pathways. This integration helps in transmitting growth-inhibitory signals. p21 interacts with proteins such as p53 and cyclins ensuring precise regulation of the cell cycle and growth arrest when necessary. Triggered by p53 activation p21 contributes to preventing undisturbed cell proliferation acting as a safeguard against tumor formation.
- Associated diseases and disorders
P21's deregulation associates with cancer and age-related diseases. Its impairment is a common feature in many cancers often linked with defects in the p53 pathway. Reduced p21 expression can contribute to unchecked cell division and tumor progression. Moreover in age-related diseases p21's role in promoting cellular senescence connects it with degenerative conditions. The intricate relationship between p21 and proteins such as p53 and cyclins highlights its potential as a therapeutic target for these disorders.
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3 product images
Sandwich ELISA - Mouse p21 ELISA Kit (CDKN1A) (ab205576)
Interpolated concentrations of p21 in mouse NIH/3T3 cell extract samples.
The concentrations of p21 were measured in duplicates, interpolated from the p21 standard curves and corrected for sample dilution. Note that 1X Diluted NIH/3T3 cell extract samples were at 80 μg/mL. The interpolated, dilution factor-corrected values are plotted in pg of p21 per μg of total protein (mean +/- SD, n=2).
Sandwich ELISA - Mouse p21 ELISA Kit (CDKN1A) (ab205576)
Camptothecin treatment of NIH/3T3 cells induces expression of p21.
NIH/3T3 cells were cultured in the absence or presence of 1 μM camptothecin for 18 hours. Raw 264.7 cells were cultured in the absence of camptothecin. The cell extracts were prepared. The concentrations of p21 were measured in the diluted cell extracts in duplicates, interpolated from the p21 standard curves and corrected for sample dilution. Note that 1X Diluted untreated NIH/3T3 cell extract samples were at 44 μg/mL. Note that 1X Diluted camptothecin treated NIH/3T3 cell extract samples were at 11 μg/mL. Note that 1X Diluted untreated Raw 264.7 cell extract samples were at 6 μg/mL. The interpolated, dilution factor-corrected values are plotted in pg of p21 per μg of total protein (mean +/- SD, n=2).
Sandwich ELISA - Mouse p21 ELISA Kit (CDKN1A) (ab205576)
Background-subtracted data values (mean +/- SD) are graphed.
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