AB205576

Mouse p21 ELISA Kit (CDKN1A)

Recombinant
SimpleStep
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(1 Publication)

Mouse p21 ELISA Kit (CDKN1A) is a single-wash 90-min Simplestep used to quantify Mouse p21 (CDKN1A) with a sensitivity of 2.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Cip1, Waf1, Cdkn1a, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein, p21

5 Images

ELISA - Mouse p21 ELISA Kit (CDKN1A) (AB205576)

ELISA

Supplier Data

ELISA - Mouse p21 ELISA Kit (CDKN1A) (AB205576)

sELISA

Supplier Data

Sandwich ELISA - Mouse p21 ELISA Kit (CDKN1A) (AB205576)

Interpolated concentrations of p21 in mouse NIH/3T3 cell extract samples.

The concentrations of p21 were measured in duplicates, interpolated from the p21 standard curves and corrected for sample dilution. Note that 1X Diluted NIH/3T3 cell extract samples were at 80 μg/mL. The interpolated, dilution factor-corrected values are plotted in pg of p21 per μg of total protein (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse p21 ELISA Kit (CDKN1A) (AB205576)

Camptothecin treatment of NIH/3T3 cells induces expression of p21.

NIH/3T3 cells were cultured in the absence or presence of 1 μM camptothecin for 18 hours. Raw 264.7 cells were cultured in the absence of camptothecin. The cell extracts were prepared. The concentrations of p21 were measured in the diluted cell extracts in duplicates, interpolated from the p21 standard curves and corrected for sample dilution. Note that 1X Diluted untreated NIH/3T3 cell extract samples were at 44 μg/mL. Note that 1X Diluted camptothecin treated NIH/3T3 cell extract samples were at 11 μg/mL. Note that 1X Diluted untreated Raw 264.7 cell extract samples were at 6 μg/mL. The interpolated, dilution factor-corrected values are plotted in pg of p21 per μg of total protein (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse p21 ELISA Kit (CDKN1A) (AB205576)

Representative image of Mouse p21 ELISA Kit (CDKN1A) (ab205576)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse p21 (CDKN1A) Capture Antibody

- 10X Mouse p21 (CDKN1A) Detector Antibody

- Mouse p21 (CDKN1A) Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Lab

Sandwich ELISA - Mouse p21 ELISA Kit (CDKN1A) (AB205576)

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 2.7 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse p21 ELISA Kit (CDKN1A) ab205576 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse p21 (CDKN1A)in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 2.7 pg/mL.

How the assay works

Mouse p21 (CDKN1A)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse p21 ELISA Kit (CDKN1A) ab205576 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab205576-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB205576-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB205576-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB205576-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB205576-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB205576-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB205576-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB205576-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse p21 Detector Antibody", "number":"AB205576-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB205576-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB205576-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse p21 Lyophilized Recombinant Protein", "number":"AB205576-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse p21 Capture Antibody", "number":"AB205576-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p21 protein also known as CDKN1A is an important regulator of cell cycle progression. It acts as a cyclin-dependent kinase inhibitor where it binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes. The molecular weight of p21 is approximately 21 kDa. Cellular expression of p21 is diverse; it occurs in various tissues reflecting its broad role in maintaining cell cycle control. This protein's expression level is often assessed using techniques such as p21 western blotting p21 ELISA and p21 IHC staining.

Biological function summary

P21 plays a critical role in cell cycle regulation and DNA damage response. It associates with the p53 tumor suppressor protein forming an essential part of the p53 signaling pathway under stress conditions. p21 arrests cells in G1 phase allowing DNA repair or leading to cellular senescence. Through these mechanisms p21 integrates signals from stress and damage emphasizing its importance as a mediator in cellular checkpoint control.

Pathways

P21 is closely involved in the p53 and the Transforming Growth Factor-beta (TGF-?) signaling pathways. This integration helps in transmitting growth-inhibitory signals. p21 interacts with proteins such as p53 and cyclins ensuring precise regulation of the cell cycle and growth arrest when necessary. Triggered by p53 activation p21 contributes to preventing undisturbed cell proliferation acting as a safeguard against tumor formation.

P21's deregulation associates with cancer and age-related diseases. Its impairment is a common feature in many cancers often linked with defects in the p53 pathway. Reduced p21 expression can contribute to unchecked cell division and tumor progression. Moreover in age-related diseases p21's role in promoting cellular senescence connects it with degenerative conditions. The intricate relationship between p21 and proteins such as p53 and cyclins highlights its potential as a therapeutic target for these disorders.

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Product protocols

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Target data

May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (PubMed : 25329316). Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (By similarity). Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (By similarity).. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed : 37178686). Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts (PubMed : 37178686). Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (PubMed : 25329316). Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (By similarity). Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes (PubMed : 36689330).

See full target information Cdkn1a

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 6: PubMed29368801

2018

Adaptive immune responses are altered in adult mice following neonatal hyperoxia.

Applications

Unspecified application

Species

Mouse

Vasantha H S Kumar,Huamei Wang,Lori Nielsen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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