Mouse p27Kip1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse p27Kip1 in Cell culture extracts, Tissue Extracts samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
15.6 - 1000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 7.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Important regulator of cell cycle progression (PubMed:8033213, PubMed:12972555). Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (By similarity). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes (PubMed:8033213). Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Alternative names
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b
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Recommended products
Mouse p27Kip1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse p27Kip1 in Cell culture extracts, Tissue Extracts samples.
Alternative names
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
15.6 - 1000 pg/mL
- Assay time
1h 30m
- Assay Platform
Microplate (12 x 8 well strips)
- Sensitivity
= 7.5 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 8 | mean - | SD - | C.V. 3.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 6.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 96 | Range 94 - 101 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse p27Kip1 ELISA Kit (ab208982) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p27Kip1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse p27Kip1 with 7.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cyclin-dependent kinase inhibitor 1B (p27Kip1) is an enzyme inhibitor that is part of the Cip/Kip family of cyclin dependent kinase inhibitor proteins. p27Kip1 is a regulator of cell cycle progression involved at the G1 stage by binding to and preventing the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
- Biological function summary
The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.
- Pathways
The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.
- Associated diseases and disorders
Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
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3 product images
Sandwich ELISA - Mouse p27Kip1 ELISA Kit (ab208982)
Example of mouse p27Kip1 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse p27Kip1 ELISA Kit (ab208982)
Interpolated concentrations of native p27Kip1 in mouse 3T3 extract and skeletal muscle extract based on a 1,000 μg/mL extract load.
The concentrations of p27Kip1 were measured in duplicate and interpolated from the p27Kip1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean p27Kip1 concentration was determined to be 496 pg/mL in NIH3T3 extract and 634 pg/mL in mouse skeletal muscle extract.
Sandwich ELISA - Mouse p27Kip1 ELISA Kit (ab208982)
This assay detects rat p27Kip1.
The concentrations of p27Kip1 in rat brain extract were measured in duplicate, interpolated from the mouse p27Kip1 standard curves and corrected for sample dilution. Undiluted samples are rat brain extract 62.5 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean p27Kip1 concentration was determined to be 1,077 pg/mL in rat brain extract.
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