AB224878

Mouse p53 ELISA Kit

Recombinant
SimpleStep
What is this?

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(5 Publications)

Mouse p53 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse p53 with a sensitivity of 44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

P53, Trp53, Tp53, Cellular tumor antigen p53, Tumor suppressor p53

6 Images

ELISA

Supplier Data

ELISA - Mouse p53 ELISA Kit (AB224878)

sELISA

Supplier Data

Sandwich ELISA - Mouse p53 ELISA Kit (AB224878)

Interpolated concentrations of native p53 in Mouse cell extract samples based on a 150 μg/mL extract load for A20, 12.5 μg/ml for MEF, and 200 μg/mL for 1 mM doxorubicin-treated (6 hours) HIN/3T3 Cell Extract. The concentrations of p53 were measured in duplicate and interpolated from the p53 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean p53 concentration was determined to be 9711 pg/mL in A20 Cell Extract, 9461 p/mL in MEF Cell Extract, and 7220 pg/mL in doxorubicin-treated NIH/3T3 Cell Extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse p53 ELISA Kit (AB224878)

Representative image of Mouse p53 ELISA Kit (ab224878)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse p53 ELISA Kit Capture Antibody

- 10X Mouse p53 Detector Antibody

- 10X Mouse p53 ELISA Kit Lyophilised Purified Protein (2 vials)

- Mouse p53 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Mouse p53 ELISA Kit (AB224878)

Example of Mouse p53 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse p53 ELISA Kit (AB224878)

Serial dilutions of 1 µM doxorubicin-treated (6 hours) and untreated NIH/3T3 cell extract starting from 200 µg/ml were assayed.

The means of interpolated, dilution factor-corrected values are plotted.

sELISA

Supplier Data

Sandwich ELISA - Mouse p53 ELISA Kit (AB224878)

Highly purified recombinant mouse, rat and human p53 were prepared at 2,500 pg/ml and assayed in duplicates.

Background-subtracted O.D values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 44 pg/mL

Range

156.3 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse p53 ELISA Kit ab224878 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse p53 in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 44 pg/mL.

How the assay works

Mouse p53 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse p53 ELISA Kit ab224878 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-p53 antibody [EPR20416-18] - BSA and Azide free (Capture/Detector) ab244935
- Anti-p53 antibody [EPR20416-124] - BSA and Azide free (Detector) ab244935

p53 acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. p53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. p53 mediated apoptosis induction seems to be by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. p53 is also implicated in Notch signaling crossover.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "MEF Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "MEF Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "111 - 122 %", "average": "= 118" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab224878-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB224878-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB224878-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB224878-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB224878-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB224878-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB224878-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB224878-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse p53 Capture Antibody", "number":"AB224878-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse p53 Lyophilized Recombinant Protein", "number":"AB224878-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB224878-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB224878-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse p53 Detector Antibody", "number":"AB224878-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.

Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.

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Product protocols

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Target data

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed : 19556538, PubMed : 20673990, PubMed : 22726440). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (By similarity). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (By similarity). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression (By similarity). Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed : 24051492).

See full target information Tp53

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39405093

2024

A new potential strategy for cutaneous squamous cell carcinoma treatment by generating serum-based antibodies from tumor-exposed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Liu

Apoptosis : an international journal on programmed cell death 28:1226-1240 PubMed37243773

2023

Effect of P53 nuclear localization mediated by G3BP1 on ferroptosis in acute liver failure.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyuan Li,Wei Li,Xun Li,Luwen Wang,Yao Wang

TH open : companion journal to thrombosis and haemostasis 7:e1-e13 PubMed36751299

2023

Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichi Hiramoto,Nobuyuki Akita,Junji Nishioka,Koji Suzuki

Pharmaceuticals (Basel, Switzerland) 14: PubMed33799790

2021

Tioconazole and Chloroquine Act Synergistically to Combat Doxorubicin-Induced Toxicity via Inactivation of PI3K/AKT/mTOR Signaling Mediated ROS-Dependent Apoptosis and Autophagic Flux Inhibition in MCF-7 Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Afnan H El-Gowily,Samah A Loutfy,Ehab M M Ali,Tarek M Mohamed,Mohammed A Mansour

Genes & cancer 11:83-94 PubMed32577159

2020

Loss of TRP53 (p53) accelerates tumorigenesis and changes the tumor spectrum of SJL/J mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jane A Branca,Benjamin E Low,Ruth L Saxl,Jennifer K Sargent,Rosalinda A Doty,Michael V Wiles,Beth L Dumont,Muneer G Hasham

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse p53 ELISA Kit

ELISA - Mouse p53 ELISA Kit (AB224878)

Sandwich ELISA - Mouse p53 ELISA Kit (AB224878)

Sandwich ELISA - Mouse p53 ELISA Kit (AB224878)

Sandwich ELISA - Mouse p53 ELISA Kit (AB224878)

Sandwich ELISA - Mouse p53 ELISA Kit (AB224878)

Sandwich ELISA - Mouse p53 ELISA Kit (AB224878)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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