Mouse p53 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse p53 with a sensitivity of 44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- Apoptosis : an international journal on programmed cell death 28:1226-12402023Effect of P53 nuclear localization mediated by G3BP1 on ferroptosis in acute liver failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyuan Li,Wei Li,Xun Li,Luwen Wang,Yao WangPubMed 37243773
- TH open : companion journal to thrombosis and haemostasis 7:e1-e132023Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeiichi Hiramoto,Nobuyuki Akita,Junji Nishioka,Koji SuzukiPubMed 36751299
- Pharmaceuticals (Basel, Switzerland) 14:2021Tioconazole and Chloroquine Act Synergistically to Combat Doxorubicin-Induced Toxicity via Inactivation of PI3K/AKT/mTOR Signaling Mediated ROS-Dependent Apoptosis and Autophagic Flux Inhibition in MCF-7 Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAfnan H El-Gowily,Samah A Loutfy,Ehab M M Ali,Tarek M Mohamed,Mohammed A MansourPubMed 33799790
- Genes & cancer 11:83-942020Loss of TRP53 (p53) accelerates tumorigenesis and changes the tumor spectrum of SJL/J mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJane A Branca,Benjamin E Low,Ruth L Saxl,Jennifer K Sargent,Rosalinda A Doty,Michael V Wiles,Beth L Dumont,Muneer G HashamPubMed 32577159
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.3 - 10000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 44 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:19556538, PubMed:20673990, PubMed:22726440). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (By similarity). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (By similarity). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression (By similarity). Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Alternative names
P53, Trp53, Tp53, Cellular tumor antigen p53, Tumor suppressor p53
What's included?
Recommended products
Mouse p53 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse p53 with a sensitivity of 44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.3 - 10000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 44 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample MEF Extract | n 5 | C.V. 2.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample MEF Extract | n 3 | C.V. 2.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 118 | Range 111 - 122 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse p53 ELISA Kit ab224878 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse p53 in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 44 pg/mL.
How the assay works
Mouse p53 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse p53 ELISA Kit ab224878 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-p53 antibody [EPR20416-18] - BSA and Azide free (Capture/Detector) Anti-p53 antibody [EPR20416-124] - BSA and Azide free (Detector) ab244935
- Anti-p53 antibody [EPR20416-124] - BSA and Azide free (Detector) Anti-p53 antibody [EPR20416-124] - BSA and Azide free (Detector) ab244935
p53 acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. p53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. p53 mediated apoptosis induction seems to be by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. p53 is also implicated in Notch signaling crossover.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
- Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
- Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.
- Associated diseases and disorders
P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
Product promise
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5 product images
ELISA - Mouse p53 ELISA Kit (ab224878)
Sandwich ELISA - Mouse p53 ELISA Kit (ab224878)
Example of Mouse p53 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse p53 ELISA Kit (ab224878)
Interpolated concentrations of native p53 in Mouse cell extract samples based on a 150 μg/mL extract load for A20, 12.5 μg/ml for MEF, and 200 μg/mL for 1 mM doxorubicin-treated (6 hours) HIN/3T3 Cell Extract. The concentrations of p53 were measured in duplicate and interpolated from the p53 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean p53 concentration was determined to be 9711 pg/mL in A20 Cell Extract, 9461 p/mL in MEF Cell Extract, and 7220 pg/mL in doxorubicin-treated NIH/3T3 Cell Extract.
Sandwich ELISA - Mouse p53 ELISA Kit (ab224878)
Serial dilutions of 1 µM doxorubicin-treated (6 hours) and untreated NIH/3T3 cell extract starting from 200 µg/ml were assayed.
The means of interpolated, dilution factor-corrected values are plotted.
Sandwich ELISA - Mouse p53 ELISA Kit (ab224878)
Highly purified recombinant mouse, rat and human p53 were prepared at 2,500 pg/ml and assayed in duplicates.
Background-subtracted O.D values (mean +/- SD) are graphed.
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