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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Mouse p53 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse p53 in Cell culture extracts, Tissue Extracts samples.
Colorimetric
Cell culture extracts, Tissue Extracts
Sandwich (quantitative)
Mouse
156.3 - 10000 pg/mL
1h 30m
= 44 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (By similarity). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (By similarity). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression (By similarity). Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Cellular tumor antigen p53, Tumor suppressor p53, P53, Tp53, Trp53
Mouse p53 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse p53 in Cell culture extracts, Tissue Extracts samples.
Cellular tumor antigen p53, Tumor suppressor p53, P53, Tp53, Trp53
Colorimetric
Cell culture extracts, Tissue Extracts
Sandwich (quantitative)
Mouse
156.3 - 10000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 44 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample MEF Extract | n 5 | mean - | SD - | C.V. 2.3 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample MEF Extract | n 3 | mean - | SD - | C.V. 2.8 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture extracts | Average % = 118 | Range 111 - 122 % |
Blue Ice
+4°C
+4°C
Mouse p53 ELISA Kit (ab224878) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p53 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse p53 with 44 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
p53 acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. p53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. p53 mediated apoptosis induction seems to be by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. p53 is also implicated in Notch signaling crossover.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Example of Mouse p53 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native p53 in Mouse cell extract samples based on a 150 μg/mL extract load for A20, 12.5 μg/ml for MEF, and 200 μg/mL for 1 mM doxorubicin-treated (6 hours) HIN/3T3 Cell Extract. The concentrations of p53 were measured in duplicate and interpolated from the p53 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean p53 concentration was determined to be 9711 pg/mL in A20 Cell Extract, 9461 p/mL in MEF Cell Extract, and 7220 pg/mL in doxorubicin-treated NIH/3T3 Cell Extract.
Serial dilutions of 1 µM doxorubicin-treated (6 hours) and untreated NIH/3T3 cell extract starting from 200 µg/ml were assayed.
The means of interpolated, dilution factor-corrected values are plotted.
Highly purified recombinant mouse, rat and human p53 were prepared at 2,500 pg/ml and assayed in duplicates.
Background-subtracted O.D values (mean +/- SD) are graphed.
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