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Mouse p53 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse p53 in Cell culture extracts, Tissue Extracts samples.

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Images

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

156.3 - 10000 pg/mL

Assay time

1h 30m

Sensitivity

= 44 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (By similarity). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (By similarity). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression (By similarity). Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

Alternative names

What's included?

1 x 96 Tests
Components
10X Mouse p53 Capture Antibody
1 x 600 µL
10X Mouse p53 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BR
1 x 6 mL
Mouse p53 Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Mouse p53 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse p53 in Cell culture extracts, Tissue Extracts samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

156.3 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 44 pg/mL

Precision

Intra assay

Sample

MEF Extract

n

5

mean

-

SD

-

C.V.

2.3

Inter assay

Sample

MEF Extract

n

3

mean

-

SD

-

C.V.

2.8

Recovery

Sample specific recovery

Sample type

Cell culture extracts

Average %

= 118

Range

111 - 122 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse p53 ELISA Kit (ab224878) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p53 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse p53 with 44 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

p53 acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. p53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. p53 mediated apoptosis induction seems to be by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. p53 is also implicated in Notch signaling crossover.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Sandwich ELISA - Mouse p53 ELISA Kit (ab224878), expandable thumbnail

    Sandwich ELISA - Mouse p53 ELISA Kit (ab224878)

    Example of Mouse p53 standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse p53 ELISA Kit (ab224878), expandable thumbnail

    Sandwich ELISA - Mouse p53 ELISA Kit (ab224878)

    Interpolated concentrations of native p53 in Mouse cell extract samples based on a 150 μg/mL extract load for A20, 12.5 μg/ml for MEF, and 200 μg/mL for 1 mM doxorubicin-treated (6 hours) HIN/3T3 Cell Extract. The concentrations of p53 were measured in duplicate and interpolated from the p53 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean p53 concentration was determined to be 9711 pg/mL in A20 Cell Extract, 9461 p/mL in MEF Cell Extract, and 7220 pg/mL in doxorubicin-treated NIH/3T3 Cell Extract.

  • Sandwich ELISA - Mouse p53 ELISA Kit (ab224878), expandable thumbnail

    Sandwich ELISA - Mouse p53 ELISA Kit (ab224878)

    Serial dilutions of 1 µM doxorubicin-treated (6 hours) and untreated NIH/3T3 cell extract starting from 200 µg/ml were assayed.

    The means of interpolated, dilution factor-corrected values are plotted.

  • Sandwich ELISA - Mouse p53 ELISA Kit (ab224878), expandable thumbnail

    Sandwich ELISA - Mouse p53 ELISA Kit (ab224878)

    Highly purified recombinant mouse, rat and human p53 were prepared at 2,500 pg/ml and assayed in duplicates.

    Background-subtracted O.D values (mean +/- SD) are graphed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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