AB323698

Mouse p53 (phospho S15) ELISA Kit

Recombinant
SimpleStep
What is this?

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Mouse p53 (phospho S15) ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse p53 (phospho S15) with a sensitivity of 56.271 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

5 Images

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

sELISA

Supplier Data

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

Interpolated concentration of native p53 (phospho S15) was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 125 µg/mL doxorubicin treated NIH/3T3 cell extract (1 μg/mL for 16 hrs). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

The concentration of p53 (phospho S15) was measured in doxorubicin treated and control NIH/3T3 cell extracts based on a 125 µg/mL extract load. Cells were treated with 1 μg/mL (1.84 μM) doxorubicin for 16 hours. The mean concentration was determined to be 8,965 pg/mL in the treated extract and undetectable (ND) in the control.

sELISA

Supplier Data

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

Example of mouse p53 (phospho S15) standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

Interpolated concentration of native p53 (phospho S15) was measured in duplicate at different sample concentrations. Undiluted sample is 125 μg/mL doxorubicin treated NIH/3T3 cell extract (1 μg/mL for 16 hrs). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

Example of mouse p53 (phospho S15) standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 56.271 pg/mL

Range

234.375 - 15000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse p53 (phospho S15) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p53 (phospho S15) protein in Cell Lysate. Quantitate Mouse p53 (phospho S15) with 56.271 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.3" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "89 - 99 %", "average": "= 94" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab323698-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB323698-CMP11" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB323698-CMP09" }, { "size":"10 x 600 µL", "name":"10X Mouse p53 (phospho S15) Detector Antibody", "number":"AB323698-CMP02" }, { "size":"10 x 600 µL", "name":"10X Mouse p53 (phospho S15) Capture Antibody", "number":"AB323698-CMP01" }, { "size":"20 x 1 Vial", "name":"Mouse p53 (phospho S15) Lyophilized Recombinant Protein", "number":"AB323698-CMP03" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB323698-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323698-CMP06" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB323698-CMP04" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB323698-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323698-CMP10" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab323698-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323698-CMP11" }, { "size":"1 x 600 µL", "name":"10X Mouse p53 (phospho S15) Detector Antibody", "number":"AB323698-CMP02" }, { "size":"1 x 600 µL", "name":"10X Mouse p53 (phospho S15) Capture Antibody", "number":"AB323698-CMP01" }, { "size":"2 x 1 Vial", "name":"Mouse p53 (phospho S15) Lyophilized Recombinant Protein", "number":"AB323698-CMP03" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB323698-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB323698-CMP08" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323698-CMP06" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB323698-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB323698-CMP04" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB323698-CMP12" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab323698-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323698-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse p53 (phospho S15) Detector Antibody", "number":"AB323698-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse p53 (phospho S15) Capture Antibody", "number":"AB323698-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse p53 (phospho S15) Lyophilized Recombinant Protein", "number":"AB323698-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB323698-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB323698-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323698-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323698-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB323698-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323698-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB323698-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed : 19556538, PubMed : 20673990, PubMed : 22726440). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (By similarity). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (By similarity). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression (By similarity). Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed : 24051492).

See full target information Tp53 phospho S15

Alternative Names

P53, Trp53, Tp53, Cellular tumor antigen p53, Tumor suppressor p53

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse p53 (phospho S15) ELISA Kit

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

Sandwich ELISA - Mouse p53 (phospho S15) ELISA Kit (AB323698)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Alternative Names

Product promise