AB197752

Mouse PAI1 ELISA Kit (SERPINE1)

Recombinant
SimpleStep
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(10 Publications)

Mouse PAI1 ELISA Kit (SERPINE1) is a single-wash 90-min Simplestep used to quantify Mouse PAI1 (SERPINE1) with a sensitivity of 200 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Mr1, Pai1, Planh1, Serpine1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

7 Images

ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

ELISA

Supplier Data

ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

sELISA

Lab

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Interpolated concentration of native PAI1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Example of mouse PAI1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Interpolated concentrations of PAI1 in mouse serum and plasma (citrate).

The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 18.98 ng/mL in mouse serum and 7.69 ng/mL in mouse plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Comparison of secreted PAI1 in unstimulated and PMA/PHA-stimulated L929 Cells.

L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. PAI1 was measured in 10-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the PAI1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 17.9 ng/mL unstimulated, 31.1 ng/mL stimulated, and undetectable in media.

sELISA

Supplier Data

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Example of PAI1 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Interpolated concentrations of native PAI1 in mouse L929 cell culture supernatant samples treated with PMA and PHA for 3 days

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 200 pg/mL

Range

298 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse PAI1 ELISA Kit (SERPINE1) ab197752 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse PAI1 (SERPINE1)in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 200 pg/mL.

How the assay works

Mouse PAI1 (SERPINE1)SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse PAI1 ELISA Kit (SERPINE1) ab197752 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse PAI1 ELISA Kit (SERPINE1) only requires a maximum of 12.5µL sample

Plasminogen activator inhibitor-1 (PAI1 or Serpin E1) is encoded by the Serpine1 gene. PAI1 is a serine protease inhibitor with a major role in the regulation of fibrinolysis. PAI1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), both of which are activators of plasminogen. PAI1 is a secreted protein produced by the endothelium as well as adipose tissue.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" } ]

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Recovery

[ { "sample": "Serum", "range": "90 - 121 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "96 - 111 %", "average": "= 104" }, { "sample": "Cell culture media", "range": "72 - 88 %", "average": "= 83" }, { "sample": "serum free media", "range": "104 - 128 %", "average": "= 117" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair (By similarity). Involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (PubMed : 28722352). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).

See full target information Serpine1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:3103 PubMed36813909

2023

Plasminogen activator inhibitor 1 is not a major causative factor for exacerbation in a mouse model of SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Takashin Nakayama,Tatsuhiko Azegami,Maki Kiso,Masaki Imai,Ryuta Uraki,Kaori Hayashi,Akihito Hishikawa,Norifumi Yoshimoto,Ran Nakamichi,Erina Sugita-Nishimura,Eriko Yoshida-Hama,Yoshihiro Kawaoka,Hiroshi Itoh

International journal of molecular sciences 24: PubMed36835405

2023

The Pre-Stroke Induction and Normalization of Insulin Resistance Respectively Worsens and Improves Functional Recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Ellen Vercalsteren,Dimitra Karampatsi,Doortje Dekens,Aikaterini Letsiou,Alexander Zabala,Mihaela Romanitan,Thomas Klein,Thomas Nyström,Vladimer Darsalia,Cesare Patrone

TH open : companion journal to thrombosis and haemostasis 7:e1-e13 PubMed36751299

2023

Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichi Hiramoto,Nobuyuki Akita,Junji Nishioka,Koji Suzuki

Cell death & disease 13:722 PubMed35982051

2022

Novel 1-hydroxy phenothiazinium-based derivative protects against bacterial sepsis by inhibiting AAK1-mediated LPS internalization and caspase-11 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Yuan,Kelong Ai,Menghua Xiang,Chengliang Xie,Mingyi Zhao,Ming Wu,Hongli Li,Yueren Wu,Yueqing Cao,Can Li,Yanjun Zhong,Xiaomeng Pei,Helen Ka Wai Law,Liqian Gao,Qicai Xiao,Xinyu Yang

Environmental pollution (Barking, Essex : 1987) 287:117283 PubMed34426376

2021

Pharmacological inhibition of PAI-1 alleviates cardiopulmonary pathologies induced by exposure to air pollutants PM.

Applications

Unspecified application

Species

Unspecified reactive species

Asish K Ghosh,Saul Soberanes,Elizabeth Lux,Meng Shang,Raul Piseaux Aillon,Mesut Eren,G R Scott Budinger,Toshio Miyata,Douglas E Vaughan

Immunity 54:454-467.e6 PubMed33561388

2021

Heparin prevents caspase-11-dependent septic lethality independent of anticoagulant properties.

Applications

Unspecified application

Species

Unspecified reactive species

Yiting Tang,Xiangyu Wang,Zhaozheng Li,Zhihui He,Xinyu Yang,Xiaoye Cheng,Yue Peng,Qianqian Xue,Yang Bai,Rui Zhang,Kai Zhao,Fang Liang,Xianzhong Xiao,Ulf Andersson,Haichao Wang,Timothy R Billiar,Ben Lu

American journal of respiratory cell and molecular biology 64:368-378 PubMed33290178

2020

Lrp1 Regulation of Pulmonary Function. Follow-Up of Human GWAS in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cody E Nichols,John S House,Huiling Li,James M Ward,Annah Wyss,Jason G Williams,Leesa J Deterding,Jennifer A Bradbury,Laura Miller,Darryl C Zeldin,Stephanie J London

Cell reports 28:3329-3337.e5 PubMed31553904

2019

SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher D Wiley,Su Liu,Chandani Limbad,Anna M Zawadzka,Jennifer Beck,Marco Demaria,Robert Artwood,Fatouma Alimirah,Jose-Alberto Lopez-Dominguez,Chisaka Kuehnemann,Steven R Danielson,Natan Basisty,Herbert G Kasler,Tal Ronnen Oron,Pierre-Yves Desprez,Sean D Mooney,Bradford W Gibson,Birgit Schilling,Judith Campisi,Pankaj Kapahi

Experimental and therapeutic medicine 15:5125-5132 PubMed29805539

2018

MicroRNA-30b protects myocardial cell function in patients with acute myocardial ischemia by targeting plasminogen activator inhibitor-1.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Jie Hu,Xingpeng Chen

International journal of molecular medicine 40:1869-1880 PubMed29039441

2017

Rivaroxaban attenuates thrombosis by targeting the NF-κB signaling pathway in a rat model of deep venous thrombus.

Applications

Unspecified application

Species

Unspecified reactive species

Junhao Ma,Xinxi Li,Yang Wang,Zhenwei Yang,Jun Luo

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse PAI1 ELISA Kit (SERPINE1)

ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (AB197752)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (10)

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